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Citations for Millipore Sigma :
2101 - 2150 of 2317 citations for 8 Fluoranthenemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Then the leaves were soaked up using absorbent paper and transferred into another square Petri dish containing 8 g L-1 agar with 50 mg L-1 benzimidazole (Sigma-Aldrich, USA) for 24 hours and finally transferred to new Petri dishes and infected with Bgt #70.
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 mL of filtered virus particles were added to the cell suspension of 50,000 cells in the appropriate media supplemented with 10% FBS and 8-10 μg/mL polybrene (Sigma # TR-1003-G) (without any antibiotics ...
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bioRxiv - Neuroscience 2022Quote: ... The recombination was induced in 8-weeks old animals by intraperitoneal (i.p.) injection of 1 mg tamoxifen (100 μl; Sigma-Aldrich, Buchs, Switzerland), dissolved in sunflower seed oil/ethanol (10:1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
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bioRxiv - Neuroscience 2022Quote: ... −1.8 mm from cortical surface) using glass microcapillary (tip diameter ≃35 μm; 1–5 μL Hirschmann® microcapillary pipette, Z611239, Sigma-Aldrich) connected to a custom Openspritzer pressure system (Forman et al. ...
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bioRxiv - Microbiology 2024Quote: ... the dried protein pellet was dissolved in 50 µL of 8 M urea (Cytiva, 17131901) in 100 mM NH4HCO3 (ABC; Sigma-Aldrich, 11213) and 5 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was either digested for 4 min for nChIP or 8 min for ChIP-seq with 1.0 U MNase (Sigma Cat #N3755-500UN) in nuclei solubilized with 1 mL of 0.1 M TE (10 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells at 70-80% confluence were infected with the viral supernatant supplemented with 8 μg/ml polybrene (Sigma: TR-1003-G). Infected cells were selected using puromycin (Meilunbio ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... 20-30 μg of protein for each sample was separated by 8-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore, cat# IPVH00010) using Trans-Blot Systems (Bio-rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing lentiviral particles was filtered through a 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Developmental Biology 2022Quote: ... WT/IWR consists of 9 wild type embryos and 8 embryos treated with the small molecule inhibitor 10µM IWR1 (Sigma Aldrich, Cat I0161). DMSO/DMH4 includes 12 embryos at 3 dpf ...
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bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
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bioRxiv - Pathology 2023Quote: ... Cells were infected with lentivirus carrying shHDAC6/shATG5 or shControl at MOI 5 for 24 hours in the presence of Hexamethidine Bromide (8 µg/mL; Sigma-Aldrich - H9268). Transduced cells were selected using puromycin (Sigma Aldrich #P9620 ...
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bioRxiv - Microbiology 2023Quote: Cellvis 8-well (Mountain View, CA, USA) coverslips were pre-coated with laminin (20 µg/mL, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 33 °C and washed twice with ddH2O ...
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bioRxiv - Molecular Biology 2023Quote: ... sonicated for 2 minutes of 2 sec on/8 seconds off cycles on ice using probe sonicator (Ultrasonic processor 500W, Sigma Aldrich, USA). 3-5 mL of 5 % NaCl was added to the samples and after thorough vortexing the phases were separated by centrifugation at 3,000 × g for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated for two weeks (treatments replenished at days 4, 8 and 11) prior to addition of 0.5% crystal violet (Sigma-Aldrich, Cat#C0775) in 50% methanol for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene_17446)] at a multiplicity of infection of 10 with 8 μg/mL polybrene (hexadimethrine bromide [Cat # 107689, Sigma Aldrich, St. Louis, MO, USA) for 24 hours before washing with PBS and replacing with fresh medium (49) ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Genomics 2023Quote: ... large tissue pieces were spun down in a microcentrifuge and the supernatant containing single cells was passed through a 70-um cell strainer fitted onto a 50-mL conical tube containing 8 mL DPBS + 1% bovine serum albumin (BSA; Sigma-Aldrich, A7906) in DPBS on ice ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... 1.8 µg/cm2) coated hanging culture inserts (Millicell Hanging Cell Culture Insert, polyethylene terephthalate, 1.0 µm pore size, EMD Millipore, MA, USA) and maintained at 37°C in 5 % CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... then transferred to a microcentrifuge tube on ice which had previously been coated with bovine serum albumin (BSA) by washing with 0.5 mL of 10% (v./v.) BSA (Sigma 9048-46-8) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Developmental Biology 2023Quote: ... the desired number of embryos were aggregated at the uncompacted 8-cell stage and cultured together in a single microdroplet of global® medium under mineral oil (Sigma, M8410). Embryo aggregation was encouraged by placing them in contact with each other in micro-indented wells in 3.5mm petri dishes (Falcon ...
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bioRxiv - Biophysics 2023Quote: ... the master SU-8 2050 photoresist was treated with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich, Saint Louis, MO) in a desiccator for overnight ...
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bioRxiv - Microbiology 2023Quote: ... Transductions were performed via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL protamine sulfate (Millipore Sigma #P3369). Cells were nucleofected with SLC35A2 RNPs two days later ...
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bioRxiv - Plant Biology 2023Quote: ... cerevisiae was isolated from liquid YPD cultures 8 hours after inoculation from single colonies using the formamide-EDTA method (59) followed by RNAzol® RT (Sigma-Aldrich) extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... viral transduction was performed by spin-infection with 2,000 g at 33 °C for 2 hours in the presence of 8 μg/ml polybrene (Sigma-Aldrich, cat# H9268), followed by incubation for another 4 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 210,000 cells each of asynchronous and mitotic cells were seeded (same density as 60,000 cells per well of the 8 chambered cover glass) onto a 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1mg/mL) coated in PBS (1 hour at 37℃ ...
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bioRxiv - Biochemistry 2023Quote: ... the glycopeptides were heat treated to inactivate the trypsin and the sample was subject to treatment with PNGase F (250U, Sigma; pH 8) followed by PNGase A (NEB ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell protein lysates were obtained by lysing cell pellets on ice in NP-40 buffer (150mM NaCl, 1% NP-40, 50mM tris pH 8) with 10x Protease Inhibitor Cocktail (Sigma-Aldrich, P8340). Protein quantification was done using the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... transferred onto nitrocellulose membrane (Amersham #10600041) in transfer buffer (8 mM Tris, 30 mM Glycine, 20 % Methanol) and stained with Ponceau S (Sigma-Aldrich, #P7170). Membranes were blocked in TBS-T + 5% BSA for 1 hr at RT and incubated overnight at 4°C in primary antibodies prepared in TBS-T + 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... drugs were diluted in AHL saline to the following final concentrations: 100 µM Picrotoxin (P1675-1G, Sigma-Aldrich, CAS 124-87-8), 50 µM CGP54626 hydrochloride (1088/10 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell infection was performed by adding lentiviral particles to cell lines in the presence of 8 μg/ml polybrene (Sigma, TR-1003) for 12 h followed by fresh media addition ...
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bioRxiv - Immunology 2024Quote: ... Concentrated virus particles solubilized in IMDM were applied to HL60 cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich #H9268). Clones expressing sgRNAs were selected with 2 μg/ml puromycin ...
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bioRxiv - Microbiology 2024Quote: ... Then 8 µL samples were spotted on a silicon disk coated with 0.01% (w/v) poly-L-lysine (Merck Millipore, Billerica, MA, USA). Cells were fixed with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... then stored in sterilized polypropylene bottles (Nalgon, Cat. No. 2330) at 8° C until filtered using 0.22 µm Sterivex filters (Merck-Millipore, Cat. no. SVGP01050) in 60 mL increments using disposable syringes ...
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bioRxiv - Microbiology 2024Quote: ... lungs were carefully removed from the hydrogel and rinsed in 0.1M PBS and transferred to clearing solution (8% sodium dodecyl sulfate, SDS, Sigma-Aldrich Inc, USA) in 0.1M PBS containing 50mM sodium sulfite (Sigma-Aldrich Inc ...
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bioRxiv - Cell Biology 2024Quote: ... E25PV4550S) and added to HeLa-TetON-WTOTC cells (65) cultured in a media supplemented with 8 μg/mL polybrene (Sigma Aldrich, H9268). 48 hours later ...
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bioRxiv - Immunology 2024Quote: ... Sub-confluent A549 cells were subsequently transduced with 2 mL of the filtered lentivirus-containing supernatant supplemented with 8 µg/mL polybrene (Sigma-Aldrich; 107689) for 24h ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G). Transduced cells were selected by either puromycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
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bioRxiv - Developmental Biology 2020Quote: ... The minced kidneys were digested for 8 minutes at 37C in 2mL of 0.25% w/v Collagenase A/1% w/v Pancreatin (Sigma-Aldrich; Cat. 101378001, P1750), with manual dissociation via pipetting through a P1000 tip every 2 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... medium was exchanged for 450 μl per well of fresh culture medium containing 8 μg/ml polybrene (Millipore cat. no. TR-1003-G), which was also used to dilute the viral stock ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells isolated from femur and tibia of C57BL/6J mice were differentiated into BMDMs for 7 – 8 days by a standard procedure (65) in DMEM (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Head extractions were incubated at increasing concentrations of the water-soluble standard cardenolide ouabain (10−8 M to 2 × 10−3 M, Sigma, Germany, O3125-1G) at 37°C under the following conditions ...