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Citations for Millipore Sigma :
2051 - 2100 of 2317 citations for 8 Fluoranthenemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Lentiviral particles were added at low MOI to HepG2 cells in the presence of 8 μg/ml of polybrene (H9268, Sigma-Aldrich) for 6 hours followed by selection in 2mg/ml G418 (10131035 ...
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bioRxiv - Microbiology 2024Quote: ... media was removed from the cells and replaced with 1 ml of mCherry lentivirus supernatant mixed with 8 ug Polybrene Infection / Transfection Reagent (Sigma-Aldrich). 24 hours after lentivirus addition ...
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bioRxiv - Cell Biology 2024Quote: Cell differentiation was conducted using previously described protocol 32 with slight modifications by using sequential inhibition of GSK-3 by CHIR99021 (8 µM, Sigma-Aldrich) and WNT by IWP-2 (5 µM ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: ... 6–8-week old B6D2F1 females were hormone primed by an intraperitoneal injection of pregnant mare serum gonadotropin (PMS, EMD Millipore) followed 46 hours later by an injection of human chorionic gonadotropin (hCG ...
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bioRxiv - Systems Biology 2024Quote: Protein solutions (supernatant from homogenized tissues and cells) were dissolved in 100 mmol/L ABC with 8 mol/L urea (Sigma-Aldrich). The proteins were reduced with TCEP (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... the medium was replaced with medium containing 2X concentrated virus medium and 8 μg/ml polybrene (Millipore Sigma, TR-1003-G). The virus-containing medium was replaced with fresh medium 24 h post transduction.
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bioRxiv - Physiology 2024Quote: ... cells were serum-deprived in DMEM/F12 containing 0.1% Ultroser G for 24h and then incubated for 8h and 24h with fresh serum-deprived medium with no secretagogue (Basal) or with 10-8 M AngII (Sigma-Aldrich), 12 mM K+ (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... At the end of the 3-hour incubation period cells were washed 3x PBS and lysed for western blot in lysis buffer (20 mM Tris pH 8, 10% glycerol, 135 mM NaCl, 0.5% NP40, 2.5 mM MgCl2, DNAse (Sigma-Aldrich, cat# DN25-1G), 1x protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... and glucose uptake was initiated by adding 2 ml serum-free and glucose-free DMEM containing 2-[1,2-3H(N)]-2-deoxy-D-glucose (DG) (NET328, specificity 8 Ci/mmol) at 1µCi/ml and 10 mM unlabeled-2-DG (Sigma, Catalog #: D-3179) to each sample in the absence and presence of 20 µM cytochalasin B (Sigma Catalog # ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Neuroscience 2020Quote: R6/1 mice were treated (from 8 to 20 weeks of age) with vehicle (90% water, 10% Polysorbate 80) or betulinic acid (50 mg/kg; Sigma-Aldrich #855057) administered by oral gavage ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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bioRxiv - Genetics 2019Quote: ... Western blots were probed using anti-GFP (Ozyme; #JL-8; 1/1,000) and anti-tubulin (to confirm equal loading) (Sigma, #DM1A, 1/5,000) antibodies ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Neuroscience 2019Quote: ... treatment consisted of an intraperitoneal injection of either the TrkB agonist 7,8-Dihydroxyflavone (7,8-DHF) (98% Sigma Aldrich CAS#: 38183-03-8) (10mg/kg ...
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bioRxiv - Neuroscience 2019Quote: ... well-fed L3 worms were mounted on 1% agarose pads with 5 mM sodium azide (Sigma-Aldrich, CAS No. 26628-22-8), whereas dauers were mounted on pads with 9 mM sodium azide (Simpkin and Coles ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Cell Biology 2020Quote: ... The following day cells were treated for 8 h with either ES DMEM-2i + DMSO or ES DMEM-2i + CHX (100 ug/ml (Sigma-Aldrich, 01810)) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1-hTERT cells were transduced with the viral supernatant from the transfections in the presence of 8 ug/mL polybrene (Millipore, Burlington, MA) for 24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the same amount of suspended cells were added into upper chambers of Corning Costar Transwell cell culture inserts (pore size 8 µm; Sigma-Aldrich, #CLS3464) with the coating of 30 μg MatrigelTM Basement Membrane Matrix (BD Biosciences) ...
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bioRxiv - Plant Biology 2021Quote: ... mixtures consisted in 2 μl of diluted template and 8 μl of FastStart Universal SYBR Green Master (Rox) (Cat. # 04913914001, Sigma-Aldrich Argentina). Reactions were set in technical duplicates or triplicates in MicroAmp® Fast Optical 48-well 0.1 mL reaction plates (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... animals at 8 weeks of age were gavaged with 100 µL of a 30 mg/mL solution of tamoxifen/corn oil (Sigma Aldrich T5648). This was done 3 consecutive days then animals were allowed to rest for 4 days before another three consecutive daily doses (6 doses over 10 days) ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Biophysics 2020Quote: ... 304 μL BPTI (4 different concentrations ranging from 5.2 to 52.6 μM) was mixed with 8 μL of the substrate Z-GPR-pNA (Sigma-Aldrich, St. Louis, MO) (5 different concentrations ranging from 0.4 to 10 mM) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant containing lentiviral particles was filtered through 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
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bioRxiv - Neuroscience 2021Quote: Microtissues were fixed within the agarose-molded wells of the tissue culture plate with cold PFA solution (4%(w/v) paraformaldehyde, 8% (w/v) sucrose) (F79-500, Sigma-Aldrich) (S7903, Sigma-Aldrich). Microtissues were fixed for 2 hours followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in a lysis buffer (20 mMTris-HCl pH=8; 137 mMNaCl; glicerol 10%) supplemented with protease inhibitor (Sigma-Aldrich, USA), and then centrifuged at 10.000g for 15min to the subsequent supernatant storage in −80°C ...
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bioRxiv - Neuroscience 2020Quote: ... Hair cells were inactivated by the flushing of 8-10 drops of 100 mg/mL neomycin sulfate (Sigma-Aldrich, St Louis, MO) through the punctured round window membrane whilst aspirating from the apex via a small hand-drilled hole ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were obtained from B6D2F1 female mice (8–10 weeks old) 48 hours after PMSG injection and were then transferred to M2 medium (Sigma-Aldrich, M7167) supplemented with 0.2 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Immunology 2019Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8-12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Immunology 2020Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8–12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Pathology 2020Quote: ... deletion of CCN2 or STOP in reporter td Tomato using Acan CreERT2 was induced at 8 weeks of age using tamoxifen (Sigma Aldrich, UK). All mice ...
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bioRxiv - Biophysics 2020Quote: ... The otolithic membrane was gently removed after 8-min enzymatic dissociation with 50 μg/mL Collagenase IV (Sigma Aldrich, St. Louis, MO). For experiments in which calcium concentration of endolymph was varied ...
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bioRxiv - Cancer Biology 2021Quote: ... we used a Boyden chamber which consists of cell culture insert containing 8-μm pore polyethylene terephthalate membrane (EMD Millipore, Burlington, MA). The inserts were seated in 24-well companion plates ...
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bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
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bioRxiv - Developmental Biology 2020Quote: Fetal hearts were collected at E11 from uninfected and CVB3 infected (E9) dams and cultured on Millicell EZ 8-well slides (Millipore Sigma PEZGS0816) in high-glucose DMEM (ATCC 30- 2002 ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...
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bioRxiv - Physiology 2021Quote: ... and used for infection into three breast cell lines in the presence of 8 μg/ml Polybrene (Sigma-Aldrich, St. Louis, MO). Infected cells were selected with 1.5 μg/ml puromycin (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... mixed in 1x PBS (8 ml per 10 mg) prepared from PBS tablets (Dot Scientific, Michigan) with deionized distilled water and filter sterilized (Millipore, Burlington, MA). Control animals were injected with an equivalent volume of saline made of 1x PBS ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...