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Citations for Millipore Sigma :
1801 - 1850 of 2317 citations for 8 Fluoranthenemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown with or without Dox (0.5 µg/ml) for 8–14 d depending on the cell line and afterwards stained with crystal violet (Sigma-Aldrich). Colony number and area was determined on scanned plates using Fiji (Image J)57,58 ...
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bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was changed to Essential 8 (E8) medium containing 100 µM sodium butyrate (NaB, Sigma-Aldrich, Cat. no. B5887). After 3 – 4 weeks ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Synthetic neotetraploids were created from six genetically distinct diploids using the mitotic inhibitor colchicine (Sigma Aldrich, CAS: 64-86-8). Details of the methodology are found in Anneberg et al ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... supernatant containing replication-incompetent lentiviruses was filtered and applied for 24 h on the target cells in a medium containing polybrene 8 μg/mL (Sigma) (78 ...
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bioRxiv - Immunology 2024Quote: Proteins were extracted in Tris buffer (Tris 20 mM pH 8, NaCl 10 mM, MgCl2 1.5 mM) 1% Triton X-100 (Sigma-Aldrich), supplemented with 1 tablet of protease inhibitors (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The concentrated retrovirus was added to 24 h-activated T cells with 8 ng/mL polybrene (#TR-1003, Sigma-Aldrich), 200 U/mL IL-2 (#212-12 ...
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bioRxiv - Cell Biology 2024Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10–8 to 3·10–5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Genomics 2024Quote: ... HepG2 cells expressing dCas9-BFP-KRAB were transduced with dJR092 library lentiviral supernatant with polybrene (8 µg/mL, Sigma-Alrich) with a targeted low infection rate of ∼10% ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... 8–9-week-old mice were intraperitoneally (IP) injected with 0.1 mL of 25 mg/mL tamoxifen (TAM; Sigma, T5648) in corn oil (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were seeded and allowed to attach overnight before incubation with the virus and polybrene (8 µg ml−1, Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei isolation was performed by adding buffer I (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.5% NP-40, 1x protease inhibitors; Sigma-Aldrich) coupled with douncer homogenization ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with the MAGE KO library (MOI < 0.5) in the presence of 8 µg/mL (final concentration) polybrene (Sigma-Aldrich). 48 h after viral transduction ...
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bioRxiv - Neuroscience 2024Quote: ... Three baseline measurements of oxygen consumption rate (OCR) were measured prior to injection of 8 μM UK5099 (Sigma-Aldrich, PZ0160), 16 μM etomoxir (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... coli cell pellets were resuspended in low imidazole buffer (25 mM Tris HCl pH 8, 25 mM imidazole, 1 M NaCl, Sigma FAST Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Microbiology 2024Quote: ... A hyaluronic acid solution was first generated by suspending 300 mg unfractionated HA sodium salt and 8 g of bovine serum albumin fraction V at pH 5.2 (Sigma-Aldrich) in 200 mL of water and slowly adjusting to pH 7.5 using 0.1 M sodium hydroxide ...
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bioRxiv - Microbiology 2024Quote: ... Blood from infected mice exhibiting 8-10% gametocytaemia was added to ookinete medium (20% FBS, 100 μM xanthurenic acid (Sigma), 24mM sodium bicarbonate ...
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bioRxiv - Biophysics 2021Quote: ... batch number: SLCC4853) and poly(ethylene glycol) (PEG 8000, Mv 8 kg/mol, batch number: MKBT7461V) were purchased from Sigma-Aldrich. 1 ...
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bioRxiv - Cell Biology 2020Quote: ... to cells together with polybrene transfection reagent at a final concentration 8-10 mg/mL (Cat# TR-1003-G, EMD Millipore). 48 h post-infection ...
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bioRxiv - Cell Biology 2020Quote: ... a polycarbonate filter (8-μm pore size) (GE Infrastructure, Fairfield, CN, USA) was coated with a solution containing 0.2% gelatin (Sigma-Aldrich Co.) and inserted between the chambers ...
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bioRxiv - Cell Biology 2020Quote: ... streptavidin-sepharose beads were resuspended in 200 µl of 50 mM ammonium bicarbonate pH 8 containing 1 µg of trypsin (Sigma-Aldrich). Samples were then incubated at 37°C overnight with mixing on a rotating disc ...
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bioRxiv - Cell Biology 2019Quote: ... perm-1 adult worms were dissected in 8-10 µL of 62% ESF 921 with 15 µm polystyrene beads (Sigma-Aldrich) on a 22 x 50 mm coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... 12-well plates of 293T or HUVEC cells were incubated with viral inoculum diluted in antibiotic and serum free DMEM containing 8 μg/mL hexadimethrine bromide (polybrene; Sigma-Aldrich). Plates were centrifuged at 800 x g for 2 hours at room temperature (61) ...
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bioRxiv - Microbiology 2019Quote: ... niger cultures grown at 28°C for 7-12 days on PDA plates were harvested using 10 ml saline tween solution (8 g l-1 NaCl and 0.05 ml l-1 Tween 80) and filtered through Miracloth (Millipore; Billerica, USA) following the protocol described in [21] ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were infected with an MOI=1 for 16 hours in ESCFBS conditions supplemented with polybrene at 8 µg/mL (Sigma-Aldrich). 24h after infection ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in a transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich)) and mixed with the previously collected concentrated virus ...
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bioRxiv - Cell Biology 2022Quote: ... AKAP450 and AKAP450/CAMSAP2 knockout RPE1 cells were infected with lentivirus and incubated in complete medium supplemented with 8 μg/ml polybrene (Sigma-Aldrich). After 24 hrs ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml deionized water, 8 mg of sucrose (A2188.1000, Axon Lab, Switzerland) and 1 mg of Amaranth dye (A1016, Sigma-Aldrich, USA), (ii ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich) and mixed with concentrated virus ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 °C and suspended in resuspension buffer (50 mM Tris-HCl, pH 8, 300 mM NaCl, 2 mg/ml lysozyme (Sigma, L6876), 1 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was thawed on ice and resuspended in 50 μl SLB/c buffer (10 mM Tris-HCL pH 8, 10 mM EDTA, 1% SDS supplemented with protease inhibitor (11836170001, Sigma-Aldrich)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Molecular Biology 2019Quote: ... in second attempt we used the library consisted of 8 random nucleotides with fixed G in the middle position: 5’-GCTCAAGCTTACTGCAGATATNNNNGNNNNTTTAGGATCCATCTAGAGTCCGA- 3’(SIGMA-ALDRICH). 15µg of the library were made double-stranded with 5µg 3’ primer (CAST1_R- ACTCTAGATGGATCCTACG or CAST2_R-TCGGACTCTAGATGGATCCTA ...
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bioRxiv - Bioengineering 2019Quote: ... 200 μL of folded reaction was purified from excess staples and buffer exchanged into 20 mM Tris-HCl pH 8.0 and 8 mM MgCl2 using a 100kDa MWCO spin concentrator (Amicon, Merck Millipore, Billerica, MA). The concentration was subsequently adjusted to 5 nM ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 40 ml of Buffer A (50 mM Tris-HCl pH 8, 500 mM NaCl) in presence of cOmplete™ EDTA-free protease inhibitor (Sigma) and lysed using an EmulsiFlex-C3 homogenizer (Avestin) ...
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...
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bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
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bioRxiv - Biochemistry 2019Quote: ... 43-48 h after induction, cells were harvested and resuspended in buffer A (50 mM Tris pH 8, 150 mM NaCl, 1% digitonin (Sigma-Aldrich), 6.4 mM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... L-lysine-13C6, 15N2 (Lys-8, #608041)) and L-arginine-13C6, 15N4 (Arg-10, #608033) stable isotope labelled amino acids (Sigma-Aldrich). Media was also supplemented with 10% dialysed FCS (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... and lysed in solubilization buffer (50 mM Tris–HCl pH 8, 1% Igepal and 1 mM EDTA) with protease inhibitor cocktail (Sigma Aldrich). The cell lysate was centrifuged at 14,000×g for 20 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatants containing lentiviral particles were used to infect MDA-LM2 cancer cells overnight in the presence of 8 μg/ml polybrene (Sigma-Aldrich). Infected cells were selected with 2 μg/ml puromycin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... U937 cells were transduced by spin infection in which cells were suspended in viral media with 8 μg/mL polybrene (Sigma Aldrich), centrifuged at 1000 x g for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... The fixative was washed away with phosphate buffered saline buffer supplemented with Tween 20 (PBST) and cells were treated for 8 min at room temperature with a solution of 2% (w/v) Driselase (Sigma-Aldrich) in 0.4 M mannitol ...