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Citations for Millipore Sigma :
2151 - 2200 of 2317 citations for 8 Fluoranthenemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... For tumor induction 8-week old mice and Cre negative Ptchf/f animals were orally administered three times with 1 mg TAM (Sigma, St Louis, MO) dissolved in 10% ethanol/corn oil ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were placed between two coverslips and STORM buffer (50 mM Tris pH 8, 10 mM NaCl, 10% glucose, 50 mM cysteamine (Sigma, 60-23-1), 0.5 mg/ml glucose oxydase (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: WT and DCLK1+ cells were plated at 3 x 10^5 cells/well into 8-well Millicell EZ chamber slides (Millipore, cat. no. PEZGS0816) overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated by 8% and 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk compound with TBST for 3h and incubated with corresponding primary antibodies and appropriate secondary antibodies before the protein bands were detected with ECL-chemiluminescent kit (ECLplus ...
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bioRxiv - Biochemistry 2021Quote: ... rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM; Sigma-Aldrich, St. Louis, MO), Atto 465-p (8 μM) ...
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bioRxiv - Biophysics 2021Quote: ... Cat #TIB-180) were subjected to UV-crosslinking and U2AF2–RNA complexes were immunoprecipitated with 8 μg anti-U2AF2 antibody (Sigma-Aldrich, Cat #U4758) and Dynabeads Protein G (ThermoFisher Sci. ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 M guanidinium chloride, 8 U/ml proteinase K, 0.5% Triton X-100, pH 8.0; all chemicals are from Sigma-Aldrich, St. Louis, MO, USA), and incubated ON at 50°C in a humified chamber.
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bioRxiv - Neuroscience 2020Quote: ... and the retina peeled off the pigment epithelium and sclera and placed receptor-side-up onto a piece of filter paper (13 mm diameter, 8 μm pore size, Millipore, Amsterdam, The Netherlands). Vacuum was applied to the other side of the filter in order to suck away the vitreous and attach the isolated retina firmly to the paper ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were infected with APOBEC3A wild-type or catalytically inactive mutant APOBEC3AE72A viral particles for 24 hours at 37°C with 8 μg/mL of polybrene (Millipore #TR-1003-G). PC9 cells were selected in 600 μg /mL of G418 (Gibco #10131035 ...
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bioRxiv - Physiology 2021Quote: Group of female B6 mice (8 wk old) was injected in oestrus stage with eCG (G4877, 5IU, Sigma Aldrich, Saint Louis, Missouri, USA) followed after 48 h by hCG (Chorulon ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were collected in serial wells of a 24 well plate in 1x PBS with 0.02% sodium azide (NaN3; Sigma-Aldrich – 26628-22-8) and stored at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Brain homogenate was digested on a rotating platform at 37° C for 30 minutes in HBSS with 28 U/ml DNase1 and 8 U/ml papain (Sigma, St. Louis, MO). The samples were triturated after 15 and 30 minutes digestion ...
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bioRxiv - Molecular Biology 2021Quote: ... Melan-ink4a cells were plated at 8.5 × 104 cells/well in 12-well plates and transduced on the next day with approximately 3 × 105 plaque-forming unit (PFU) of lentiviral particles in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich, Steinheim, Germany). After 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... 48 hour supernatants were centrifuged and viral pellets were resuspended in 0.45 µm filtered 72 hour media and supplemented with 8 µg/mL (EMD Millipore, Burlington, MA, USA) added to either SW872 or NIH 3T3 cells overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.105 Met-5A cells were plated in Ibidi µ-Slide 8 Well (Clinisciences #80826) coated with collagen type I (Sigma-Aldrich #C3867-1VL) and incubated for 48h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Three days after the SVF media was replaced with media from the transfected 293T cells that was filtered and mixed at a 1:1 ratio with standard SVF media and viral media with added polybrene (8 µg/mL, Millipore Sigma TR-10030). The infected cells were selected for using Geneticin (G418 Sulfate ...
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bioRxiv - Biochemistry 2022Quote: ... were dissected from E13.5 wild-type mice and plated in a 8-wells chamber coated with 10µg/ml poly-d-lysin (Sigma-Aldrich, St Louis, MO) and 10µg/ml mouse Laminin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Cancer Biology 2020Quote: Ovarian cancer cell lines (5 × 104 cells per 24 well) in complete media containing 8 μg/mL Polybrene (Millipore-Sigma: TR-1003-G) were transduced with high-titer Luc-mCherry lentivirus (64) ...
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bioRxiv - Immunology 2020Quote: ... the synthesized mRNA was isolated from the reaction mix by precipitation with half volume of 8 M LiCl solution (Sigma-Aldrich, Hamburg, Germany), and finally the pellet was dissolved in nuclease-free water ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Balb/C mice at 8 weeks of age received a single iv injection of ADR (10.5 mg/kg, Sigma Aldrich, St. Louis, MO) via tail vein injection and were followed for a total of 6 weeks (48) ...
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bioRxiv - Immunology 2021Quote: Samples obtained from in vitro proteolysis assay were analyzed by electrophoresis on an 8% SDS-polyacrylamide gel followed by transfer to Immobilon polyvinylidene difluoride membranes (Millipore Co., Bedford, MA). The membranes were blocked using 4% non-fat milk and immunostained with specific mAb ...
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bioRxiv - Cell Biology 2022Quote: ... Viruses were added to cells in a 1:1 ratio with DMEM supplemented with 10% FBS with 8 μg/mL polybrene (H9268-5G; Millipore Sigma, Darmstadt, Germany). For MRC-5 ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with the wash buffer (20mM Tris pH 8, 300 mM NaCl, 20mM imidazole [Sigma-Aldrich; I5513], 1 mM DTT) three times and eluted with the elution buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Worms were cultured in 6 well dishes (4-5 mature male worms or 8-10 immature worms in 3mL media per well) at varying concentrations of praziquantel (PZQ, Sigma-Aldrich cat. # P4668) or DMSO vehicle control and imaged to record phenotypes.
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2020Quote: ... sections were treated with Weigert’s Iron Hematoxylin for 8 min to stain nuclei and Direct Red 80 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 1 hour at room temperature to visualize collagen before washing with 0.5% glacial acid ...
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bioRxiv - Neuroscience 2019Quote: ... Pregnant Wistar rats (293.0–428.7 g) therefore received a single intraperitoneal (i.p.) injection of either poly (I:C) (n=8; P9582, potassium salt, Sigma-Aldrich; Gillingham, Dorset, United Kingdom) at a dose of 10 mg/kg or 0.9% non-pyrogenic sterile saline as a control (n=8 ...
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bioRxiv - Physiology 2020Quote: ... 8-12 week old mice (males and females) were injected IP with D-gal (Sigma Aldrich, Cat# G0500, 350 mg/kg body weight) and LPS (ENZO life sciences ...
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bioRxiv - Cell Biology 2019Quote: ... The filter was then washed with 8 ml of 1% BSA PBS (diluted from SRE0036 SIGMA in CaCl/MgCl free HPBS + Rnase Inh) and nuclei suspension spun in a precooled (4°C ...
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bioRxiv - Cell Biology 2020Quote: Confluent primary mouse keratinocytes in 10-cm petri dishes were washed twice with ice-cold PBS followed by incubation with 8 ml of Biotin-NHS (Sigma, cat. no. 203112) in PBS (0.25 mg/ml ...
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bioRxiv - Physiology 2020Quote: Bone marrow from Winnie and control mice tibiae (n=4-8 mice/group) was collected by flushing with α-modified essential medium ((α-V1EV1, Sigma, Australia). Bone marrow-derived mesenchymal stromal cells (BM-MSCs ...
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bioRxiv - Cancer Biology 2021Quote: The migration of two TNBC cells (MB231, MB468) were performed using a 24-well transwell chambers (8 µm pore size; Millipore, Billerica, MA, USA). The cells were plated in triplicates into the upper chamber (1 × 105 cells/well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 8-(4-chlorophenylthio) adenosine-3’,5’-cyclic monophosphate (CPT-cAMP; PKA activator) or PMA (PKC activator)) were purchased from Sigma-Aldrich (ON, Canada). Powdered CBD ...
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bioRxiv - Cell Biology 2020Quote: ... 3T3-L1s were changed to serum-free DMEM supplemented with 0.2% BSA FFA-free (Sigma-Aldrich, St. Louis, USA, Catalog 9048-46-8). The media was supplemented with 1% DMSO for negative control or 1uM isoproterenol to induce lipolysis +/- 100 μM Atglistatin (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... were performed prior to incubation for 8 h with biotinylated mouse monoclonal antibody 4G8 (2 μg/ml, Sigma-Aldrich SIG-39240 Beta-Amyloid). Staining was completed with the Ventana XT DABMap kit and a haematoxylin counterstain ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
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bioRxiv - Bioengineering 2020Quote: Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of thawed filtered lentivirus was used to transduce approximately 2.25×106 HFF-T or 3×106 RPE cells in 10 cm plates with 8 µg/mL polybrene (Millipore-Sigma: TR-1003-G). Cells were allowed to reach confluence before selection with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were separated on 8% SDS-PAGE gels and transferred to a PVDF membrane (immobilon FL; Sigma-Aldrich, St Louis, MO, USA). Membranes were blocked with 1% (for TMEM16A protein ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The beads were then removed by magnetic separation and cells were spinoculated with HIV-GFP-Thy1.2 (pNL4-3-−Δ6-dreGFP-CD90) and 8 ug/mL polybrene (Millipore Sigma; TR-1003-G) for 2 hrs at 1100 x g ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated DA neurons were perfused with external solution containing CPA for more than 8 min and then additionally exposed to 20 mM caffeine (Sigma-Aldrich, USA, C0750) to confirm the complete depletion of ER Ca2+ ...
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bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
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bioRxiv - Neuroscience 2022Quote: ... Hydrogel was prepared separately as solution I and II: Solution I was prepared by dissolving 8 g (16% (wt/vol)) paraformaldehyde (Sigma-Aldrich, Steinheim, Germany) (PFA ...
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bioRxiv - Plant Biology 2022Quote: ... Each RNA sample was extracted from 800 μl (8 IP-Star tubes) of RNA IP product using 1 ml of TriReagent (Sigma-Aldrich T9424, USA) as indicated by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... were dosed 2 mL/Kg intraperitoneally (IP) twice weekly with either olive oil (N=8) or CCl4 (CCl4:olive oil, 1:3, [v:v], Sigma-Aldrich, St Louis, MO) for 4 weeks ...