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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Molecular Biology 2023Quote: Cells were transfected with plasmid DNA using Gene Juice Transfection Reagent (Merck Millipore) 24 h after plating ...
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing the desired genes were selected using 2 μg/ml puromycin (Sigma). An HTLA cell line stably expressing a tTA-dependent luciferase reporter and the β-arrestin2-TEV fusion gene used in the Tango assay36 was a generous gift from Bryan L ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection was carried out using the XTREME gene transfection reagent (Millipore sigma) and Opti-MEM media (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... primary rat neurons were washed with TM and then incubated with phenol red free TM containing WST-1 reagent (Sigma, Cat# 5015944001, 20 μl/ml) for 4 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panel 1 (EMD Millipore Corporation, Charles, MO), and analyzed according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 12] Urine samples were aliquoted into 96-well plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Bead Panels 1 and 2 (EMD Millipore Corporation, Charles, MO), prepared and analyzed according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RIP assay and RNA pull down assay were performed using RIP RNA-Binding Protein Immunoprecipitation kit (Sigma, USA) and Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The extraction of nuclear proteins has been performed using the CelLytic™ NuCLEAR™ Extraction kit (Sigma-Aldrich) complemented with PIC ...
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bioRxiv - Physiology 2020Quote: ... Subcellular fractions were extracted using differential lysis and solubilization with a commercial Millipore Compartmental Protein Extraction Kit (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lactate and pyruvate were measured in the protein-depleted flow-through using assay kits (MAK064-1KT, Sigma-Aldrich) and (ab65342 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant and standards containing bovine serum albumin (BSA) were analyzed using a BCA protein assay kit (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...
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bioRxiv - Immunology 2020Quote: ... The pro-inflammatory cytokines in the supernatant were analyzed by multiplex ELISA (Millipore, HCYTMAG-60K-PX29) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Nunc 96-well ELISA plates were coated with 50 µl of 2 µg/ml SA (Sigma) in borate saline buffer (100 mM boric acid ...
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bioRxiv - Neuroscience 2022Quote: ... sandwich ELISA of tau-5 coating antibody (20 µg/ml; Millipore Cat# 577801-100UG RRID: AB_212534) with either biotin-conjugated HT-7 (for human tau ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed in ELISA wash buffer (0.01M PBS with 0.05% Tween 20; Sigma; P7949) for 4x 2 minutes on an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Neuroscience 2021Quote: ... 500µL of conditioned media was collected from each 1mL well for AlphaLISA assay and macrophages were loaded with MitoSOX™ Red (Sigma Aldrich, St. Louis, Mo) mitochondrial superoxide indicator at a concentration of 1µM in complete ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 monocytes were stimulated to differentiate into macrophage-like cells by treatment with 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich Co., St Louis, MO) for 18-20 h at 37°C and incubated further for 48 h to allow differentiation (Bhaskar et al. ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Bioengineering 2020Quote: ... of 0.3 with Edit-R lentiviral mKate2-tagged, constitutively expressed Cas9 nuclease under the hEF1α promoter (Dharmacon, CAS11229) in transduction medium containing 5 μg/mL polybrene (EMD Millipore, TR-1003-G). Complete growth medium was added to the transduced cells at a 3:1 dilution after 5 hours ...
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bioRxiv - Microbiology 2023Quote: ... The hemolysis percentage was expressed in relation to a 100% lysis control (erythrocytes incubated with 0.1% triton X-100) (Sigma-Aldrich, St. Louis, MO, USA); PBS was used as negative control ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and Zbtb16 (1:250, Santa-Cruz, Mississauga, ON) Beta-actin was used as a reference protein (1:5000, Sigma-Aldrich, Oakville, ON). Blots were scanned by Amersham Imager 600 imaging system (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...