Labshake search
Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: Synthetic genes codon-optimized for Escherichia coli were cloned into the pET15b vector (Novagen) with an N-terminal hexahistidine (His6)-tag ...
-
bioRxiv - Cell Biology 2023Quote: ... Peroxisome and trafficking pathway gene primers were designed by NCBI and purchased from Sigma. The housekeeping gene GAPDH was used as normalizing control to calculate the fold change.
-
bioRxiv - Immunology 2022Quote: ... We synthesized the dTrabid gene with codon optimization to Spodoptera frugiperda (Sigma, UK Suppl) and cloned into HindIII and KpnI digested poPINS plasmid ...
-
bioRxiv - Neuroscience 2023Quote: Fragments of eaat genes were PCR amplified using a JumpStart Taq polymerase (Sigma-Aldrich) and the primer pairs eaat5b_135_s 5’-GGAGCAGGAAGTCAAGTA-3’ with eaat5b_600_as 5’-GTCCGTCCCATTATCGTC-3’ and eaat7_142_s 5’-GTAATAGCAGGCACAGTGATG-3’ with eaat7_1001_as 5’-CCCAGAGCAGTGATCCAAG-3’ ...
-
bioRxiv - Cell Biology 2023Quote: ... Gene expression was induced by addition of 0.01–0.3 µg/ml doxycycline (Sigma-Aldrich).
-
bioRxiv - Molecular Biology 2023Quote: ... Gene expression was induced by addition of 0.3 µg/ml of doxycycline (Sigma-Aldrich) for 24 h.
-
bioRxiv - Developmental Biology 2023Quote: ... The genes of interest were PCR amplified using JumpStart REDTaq ReadyMix (Sigma-Milipore, P0982) according to the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
-
bioRxiv - Synthetic Biology 2023Quote: ... The selected genes were incorporated into a modified pET28a(+) vector (NovagenTM, Sigma-Aldrich Co.) encoding the tobacco etch virus (TEV ...
-
bioRxiv - Plant Biology 2024Quote: ... Synthesized genes were subcloned from the pUC57 cloning vector into the pET-15b (Novagen) expression vector using 1.5 μg of DNA and NdeI and BamHI in 30 μl reactions ...
-
bioRxiv - Molecular Biology 2024Quote: The siRNAs used for gene knockdown experiments were chemically synthesized (Sigma-Aldrich, Shanghai GenePharma). The sequences of siRNAs are shown in Table S1.
-
bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
-
bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
-
bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation assay was carried out according to the Magna RIP RNA-Binding Protein Immunoprecipitation Kit manufacturer’s instruction (Millipore). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: Protein co-localization was assessed via proximity ligation assay (PLA) using the Duolink In Situ Red Starter Kit (Sigma) and PLA-approved primary antibodies for SC-35 (Abcam ...
-
bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
-
bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
-
bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
-
bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
-
bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
-
bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
-
bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
-
bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
-
bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
-
bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
-
bioRxiv - Microbiology 2019Quote: ... U937 cells stably expressing Grx1-roGFP2 in the cytosol were seeded at a density of 0.2 million per well in 24-well plates and were differentiated into macrophages by a 24 h treatment with 5 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich Co. Saint Louis, MO, USA). Cells were rested overnight following chemical differentiation to ensure that they reverted to a resting phenotype before infection ...
-
bioRxiv - Cell Biology 2022Quote: 96-well ELISA plates were coated overnight at 4°C with anti-mouse total β1-integrin (Millipore) or anti-mouse β1-integrin ...
-
bioRxiv - Physiology 2021Quote: ... Total and high-molecular weight adiponectin was measured by ELISA (EZMADP-60K; EMD Millipore and MBS028367; MyBiosource).
-
bioRxiv - Neuroscience 2023Quote: ... basal plasma (collection prior to IPGTT) leptin was measured using a leptin ELISA (Millipore Sigma; #EZRL-83K) following manufacturer’s instructions in duplicate ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The rHA proteins have a molecular weight of ∼270 kDa and were expressed in HEK-293 cells and purified using IMAC Sepharose® High Performance resin (Sigma Aldrich, St. Louis, MO). Viruses were from the Influenza Reagents Resource (IRR ...
-
bioRxiv - Biophysics 2022Quote: ... Growing cultures were sequenced (Sanger Sequencing, Microsynth Seqlab, Göttingen, Ger) and expressed in 500 ml LB Medium (Sigma-Aldrich Co., St. Louis, MO, USA, 51208) for 5 hours before harvesting ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
-
bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... For the time course shown in Fig 2B 19 μl of this protein were combined with 1 μl of trypsin (Sigma; 1 mg/ml) and the mixture was incubated at RT ...
-
bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
-
bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
-
bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-phospho-Histone H3 (pSer28) (clone HTA28) (Sigma Cat#H9908, RRID: AB_260096), mouse monoclonal anti-Human Nuclear Antigen (clone 235-1 ...
-
bioRxiv - Biophysics 2021Quote: ... and the e-cadherin with monoclonal rat anti-e-cadherin (Sigma, catalogue no. U3254) primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma ...
-
Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... XF24 cell culture microplates were coated with Rat Tail Collagen I (Sigma Cat#C3867) before plating wells with prepared SVF as described previously ...
-
bioRxiv - Immunology 2021Quote: ... and then incubated with a secondary anti-Rat IgG peroxidase-conjugated antibody (Sigma-Aldrich), diluted 1:5000 in 5% skim milk ...