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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was briefly vortexed in the presence of 1% Triton X-114 (Sigma), incubated on ice for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Flag-hSpt16 proteins were incubated with 12.5 U of recombinant ULP-1 SUMO Protease (Sigma) for 16 h at 4°C to remove SUMO moieties.
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of protein was incubated with 20 mM methoxypolyethylene glycol maleimide (mPEG, Sigma-Aldrich) in the presence of 3% SDS for 10 minutes at 60ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... After protein transfer blots were blocked in 5% BSA/TBS for 1 h and stained for Sox2 (1:100, Millipore AB5603), Sox9 (1:250 ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... KLH protein was dissolved in PBS to 5 mg/mL of and then mixed 1:1 with CFA (both Sigma-Aldrich) upon which 40 μL of the immunization mixture was injected in footpads and flanks of draining popliteal and inguinal LNS ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to a 1 mg/mL protein concentration using 50 mM ammonium bicarbonate and incubated overnight with trypsin (1:200 v/v, Sigma #T6567). Digested samples were acidified and urea removed using a Sep-Pak C18 96-well plate (Waters #186002320) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP and Iba-1 protein analyses were generated from previously collected data and normalized to GAPDH (1:5000; cat# G9545, Sigma-Aldrich) [13] ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Immunology 2021Quote: ... the amplified IL-22 gene was cloned into a pET32a (+) vector (Novagen, USA) using two restriction sites as shown in Figure 1b ...
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bioRxiv - Developmental Biology 2021Quote: ... All additional recombinant genes were cloned into pFLAG-CMVTM-6a expression vector (Sigma) unless otherwise specified ...
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bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2021Quote: ... the mCherry gene was amplified together with the gap1 promoter using primers (Sigma) mentioned in supplementary table 1 and the pRed plasmid (Zacchetti et al ...
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ST6GAL1 gene shRNA clone was obtained from MISSION shRNA library (Sigma Merck, USA). Plasmid containing shRNA or scrambled control was packaged into lenti virus using packaging vectors pMD2.G and psPAX2 (packaging vectors were a kind gift from Dr ...
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bioRxiv - Neuroscience 2020Quote: ... Cells expressing the target genes were selected using 2 μg/ml Puromycin (Sigma). An HEK293 cell line stably expressing a tTA-dependent luciferase reporter and the β-arrestin2-TEV fusion gene used in the TANGO assay was a generous gift from Bryan L ...
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bioRxiv - Synthetic Biology 2021Quote: ... The gene was inserted between NcoI and BamHI sites of pCDF-1b (Novagen) vector using the primers sfp-fw and sfp-rv (Supplementary Table4) ...
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bioRxiv - Plant Biology 2021Quote: ... the coding sequences (CDS) of genes were cloned into the modified pET21b (Novagen) expression vector after adding a cleavage site for the tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... The genes were inserted into the NdeI and XhoI sites of pET30b (Novagen) and the reconstructed expression vector was transformed into the expression host BL21(DE3) ...
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bioRxiv - Microbiology 2022Quote: ... the synthesized genes were cloned into pET 24a(+) (Novagen-Merck KGaA, Darmstadt, DE) and inserted between BamHI (at the 5’ end of the gene ...
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bioRxiv - Neuroscience 2022Quote: ... 400nM of each gene specific primer HPLC purified (Sigma-Aldrich, St. Louis, MO) in 25 μL reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... The synthetic gene was cloned into a pET-21a(+) (Novagen, Watford, United Kingdom) vector using NdeI and BamHI restriction sites ...
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bioRxiv - Developmental Biology 2019Quote: ... Gene expression analysis was performed using KiCqStart SYBR Green predesigned primers (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: HEK 293T cells were transiently transfected using X-treme gene 9 (Sigma Aldrich). After 48 hours of the transfection ...
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bioRxiv - Cell Biology 2019Quote: For c-fos gene activation phorbol-12-myristate-13-acetate (524400, Sigma-Aldrich) in final concentration 1 ug/ml was applied for 2h to DLD-1AID-NG-Tpr cells prior to c-fos RNA visualization.
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bioRxiv - Biochemistry 2019Quote: The human H3.1 and H3.3 genes were inserted in the pET21a vector (Novagen), as C-terminal epitope-tag-His6 fused genes ...
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bioRxiv - Biophysics 2020Quote: ... After stepwise gene amplification with increasing concentrations (0.0–10 nM) of MTX (Sigma), a high titer clone was then adapted to SFM-4CHO serum-free medium (GE BioSciences) ...
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bioRxiv - Biophysics 2020Quote: ... After stepwise gene amplification with increasing concentrations (0.0–10 nM) of MTX (Sigma), clones producing the highest S-Trimer titer were adapted to SFM-4CHO serum-free medium (GE BioSciences) ...
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bioRxiv - Immunology 2020Quote: ... After stepwise gene amplification with increasing concentrations (0.0–10 nM) of MTX (Sigma), the clones producing the highest S-Trimer titer were then adapted to SFM-4CHO serum-free medium (GE BioSciences) ...
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bioRxiv - Immunology 2020Quote: ... After stepwise gene amplification with increasing concentrations (0.0–0.5 μM) of MTX (Sigma), clones producing the highest HA-Trimer titers were then adapted to SFM-4-CHO ...
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bioRxiv - Immunology 2020Quote: ... After stepwise gene amplification with increasing concentrations (0.0–10 nM) of MTX (Sigma), a high titer clone was then adapted to SFM-4CHO serum-free medium (GE BioSciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... Gene deletion was achieved by intra-peritoneal injections of 100µg Tx (Sigma, T5648) into Smad4iΔEC ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were transfected using xTreme gene HP DNA Transfection Reagent (Sigma, 6366236001). The transfection mixture contained 100 μL 1X PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Both plasmids carry a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Vegetative growth was started from frozen aliquots thawed every week to prevent the accumulation of undesired mutations due to prolonged culturing ...
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bioRxiv - Molecular Biology 2023Quote: The Gene Switch system was activated by adding the RU486 (Sigma-Aldrich #M8046) activator to the fly food ...