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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
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bioRxiv - Immunology 2023Quote: ... was expressed and secreted from a stable S2 cell line (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich) (Wright et al. ...
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bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were diluted to 1 M urea with 50 mM TEAB buffer and digested with 1:100 trypsin (Sigma) 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature with an antibody against mHTT protein (1-82 aa, Millipore, MAB5492 1:500), and either NeuN (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of extracted protein was transferred to Eppendorf tubes to incubate with anti-FLAG-antibody (1:1000; Millipore Sigma) or anti-HIS-antibody (1:300 ...
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bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara, CA, USA; monoclonal 1:1000, MAB 3402, Millipore, Burlington ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Cell Biology 2022Quote: ... Leptin and NPY were measured using commercially available ELISAs (all from Millipore, Temecula, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ELISA was performed using conditioned media (∼80% confluent cells, concentrated with Millipore UFC 900324 filters) and normalized by protein concentration (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed and blocked with 5% Foetal Bovine Serum (FBS, Sigma, F9665) in 1x PBS/0.1% Tween 20 for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Synthetic Biology 2023Quote: ... were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) for 1hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp ELISA plates were precoated with dsDNA (100 mg/mL, Sigma, St. Louis, MO) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The protein content was measured using the BCA Kit (Bicinchoninic Acid, Sigma-Aldrich, St Louis, MO, USA) and bovine serum albumin (BSA ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of Wt and mutant HelR proteins was determined using the colorimetric ATPase assay kit (Sigma). The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The ECM fraction was isolated from the frozen tissues using the Compartment Protein Extraction Kit (Millipore, #2145) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were extracted from residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Metabolite profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under the control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...
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bioRxiv - Microbiology 2019Quote: ... 100 µl of a THP-1 cell suspension (106 cells/mL) in 20 mg/mL collagen from rat tail type I (Sigma Aldrich, St. Louis, USA) were spread in the basolateral surface of inverted inserts ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study for immunoblotting were: rat anti-mouse MLKL (WEHI clone 5A6; produced in-house and soon available from Millipore as MABC1634; 1:2000)49 ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
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bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... the protein was quantified by PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Rockford, IL, USA) and the carbohydrate was quantified by Total Carbohydrate Assay Kit (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2021Quote: ... on plates coated with Collagen Type I Rat Tail (Sigma-Aldrich, #C7661). Passages 4-6 were used for experiments ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (chicken anti-TH, Abcam #76442; Rat anti DAT, Millipore MAB369) were diluted 1:1000 in washing buffer containing 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2019Quote: ... rats were decapitated after deep anesthesia with halothane (Sigma, St. Louis, MO), the cerebellum was gently removed and the vermis was isolated ...
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bioRxiv - Plant Biology 2019Quote: ... and a goat anti-rat horse peroxidase conjugated secondary antibody (Sigma-Aldrich) (1:4000 dilution) ...
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bioRxiv - Neuroscience 2021Quote: ... all rats were pretrained with 2 bottles containing 0.2% sodium saccharin (Sigma). First ...
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bioRxiv - Neuroscience 2020Quote: ... rats had access for 15 min to almond- (0.01% benzaldehyde; Sigma Aldrich) or banana-scented (0.01% isopentyl acetate ...
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bioRxiv - Neuroscience 2020Quote: Mice and rats were euthanized with sodium pentobarbital (60 mg/mL; Sigma) and transcardially perfused with room temperature 0.9% saline followed by ice-cold 4% paraformaldehyde (PFA ...