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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were stained using primary antibodies against β-actin (1:10,000, AC-15, Sigma), strep II-tag (1:1,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used included anti-rabbit glial fibrillary acidic protein (GFAP, Sigma, 1:400), mouse anti-Zrf1 (Gfap ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole cellular protein was extracted with RIPA buffer containing 1 mM Na3VO4 (Sigma-Aldrich) and protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was concentrated to 5 mg·ml−1 in an Amicon ultracentrifugation device (Millipore) with 10 kDa cutoff and directly used for further studies or supplemented with 40% glycerol ...
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bioRxiv - Neuroscience 2020Quote: Mouse anti-Myelin Basic Protein (MBP-SMI-99, Millipore, reference NE1019, 1/1000 dilution), rat anti-Myelin Basic Protein (BIO-RAD ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies used were rabbit anti-prosurfactant protein C (SPC) (Millipore, AB3786, 1:500) and goat anti-IL-33 (R&D ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Purkinje cell protein 4 (PCP4; Sigma, dilution 1:400), rabbit polyclonal antibody raised to Capn15 (Zha ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic proteins were detected mouse anti-PSD95 (1:500, MAB1598, Millipore, Burlington, MA, U.S.A.), guinea pig anti-VGLUT1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Soluble proteins were then incubated with 1 μg of anti-FLAG antibody (F1804, Sigma) or 1 μg of mouse IgG (sc-2025 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
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bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with trypsin (1:50 µg/µg) (Sigma-Aldrich) and lysyl endopeptidase (1:75 µg/µg ...
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bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Biochemistry 2023Quote: ... Expression of chaperone protein was induced with 1 μg/ml tetracycline (Sigma-Aldrich, T7660).
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Microbiology 2019Quote: Macrophage-like phenotype was obtained by treating cells with 25 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, St-Louis, USA) for 48 h in 24-wells culture plates (Corning ...
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bioRxiv - Microbiology 2024Quote: ... Monocyte-derived macrophages were derived by treating the cell lines with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma, St. Louis, MO) for 48 h followed by a 24 h incubation in medium alone before experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Microbiology 2021Quote: ... the target gene was connected to the expression vector pET-28a (Novagen) and transformed to Escherichia coli (E ...
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bioRxiv - Microbiology 2019Quote: ... the siia gene was cloned into a pET15b vector (Novagen, Darmstadt, Germany). The translated gene product consists of an N-terminal hexa-histidine tag (MGSSHHHHHH ...
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bioRxiv - Cancer Biology 2020Quote: Transient gene-silencing experiments were performed with endoribonuclease-prepared siRNAs (esiRNA, Sigma) for GFPT1 and GFPT2 ...
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bioRxiv - Microbiology 2020Quote: ... The rexA and rexB genes were inserted into the pET28b+ vector (Novagen) using standard cloning techniques and site-directed mutagenesis (SDM ...
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bioRxiv - Microbiology 2020Quote: ... pneumoniae UKP8 was constructed using the TargeTron Gene Knockout System (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 200nM of gene specific primer- HPLC purified (Sigma-Aldrich, St. Louis, MO) in 25 μL reaction volume ...
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bioRxiv - Neuroscience 2020Quote: ... and the transfer vector using Xtreme gene 9 transfection reagent (Sigma-Aldrich). Two days after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... The primers used for the genes were KiCqStart SYBR green primers (Sigma) and the primers for miRNAs are listed in Table-2.
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bioRxiv - Bioengineering 2021Quote: Thermobifida fusca native Cel5A53,54 (UniprotKB - Q01786) gene was cloned into pET28a(+) (Novagen) and was kindly provided by Nathan Kruer-Zerhusen (from late Prof ...
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bioRxiv - Microbiology 2019Quote: The blaMm3 gene was cloned into the pET28a(+) expression vector (Novagen, USA) with N-terminal His Tag and inducible T7 promoter using the Ndel and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: The genes encoding GlxChiB and its mutants were cloned into pET22b (Novagen) at the NdeI and BamHI restriction sites ...
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bioRxiv - Biophysics 2020Quote: ... The GLuc gene named GLuc-TG [13] was inserted into pET21c (Novagen) at the NdeI/BamHI site to construct p21GLucTG with ampicillin resistance.
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bioRxiv - Immunology 2020Quote: ... Amplified S-gene and polymerase chain reaction (PCR) engineered pET31b(+) (Novagen, Germany) bacterial expression vector were amplified using 0570F and 0571R primers ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides used for gene cloning or screening purposes were purchased from Sigma and their sequences are listed in the S3 Table ...
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bioRxiv - Biochemistry 2023Quote: ... The gene was cloned into the pET11 expression vector (Novagen, Merck, Germany) using BamHI and NdeI restriction sites ...
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bioRxiv - Cell Biology 2023Quote: ... Gene deletion or overexpression were induced by injection of tamoxifen (Sigma T5648) diluted in corn oil (Sigma C8267) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting gene products were assembled into pET28a expression vector (Novagen, 69864) using Gibson assembly (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... a SYBR-green qPCR reaction was performed with gene-specific primers (Sigma) as per Nolbrant et al ...
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bioRxiv - Microbiology 2024Quote: ... Amplified genes were digested and inserted into the pET28b(+) vector (Merck Millipore) (NDK ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Microbiology 2022Quote: The His6-eVP40-pET46 expression vector was a kind gift from Erica Ollmann Saphire (La Jolla Institute for Immunology) and was expressed and grown in RosettaTM 2 BL21 DE3 competent cells (Merck Millipore, Billerica MA). The pet28a-His6-Lact C2 bacterial expression plasmid was a kind gift from Dr ...