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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM of isopropyl-β-D-thiogalactoside (IPTG) (Sigma Aldrich). Expression was continued in a bacterial shaker at 30°C and 150 rpm for 6h ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... the culture medium was supplemented with 1 μM Rho-associated protein kinase (ROCK) inhibitor (Y27632, Millipore). Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μg of proteins were mixed with 0.004% Bromophenol blue and 1% β-Mercaptoethanol (Sigma-Aldrich), incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... with the protein aqueous solution (1 mg/ml of PLL, Sigma-Aldrich, in PBS pH 10.4), at a 1:2 ratio ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 1% FBS (v/v) and protein expression enhancing sodium butyrate (65) solution (SIGMA-ALDRICH) to a final concentration of 2 nM were added to the cells to make up to the final volume of transfection ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... urine samples were aliquoted into 96-well black plates supplied with MILLIPLEX® MAP Rat Kidney Toxicity Magnetic Beed Panel 1 (EMD Millipore Corporation, Charles, MO, USA), prepared and analyzed per manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2019Quote: ... All transfections have been performed using the Gene Juice® reagent (EMD Millipore #70967) following manufacturer instruction at day 4 of hiPSC culture.
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bioRxiv - Synthetic Biology 2020Quote: Genes encoding AceCas9 and the sgRNA were inserted into the pACYCDuet vector (Novagen 71147) to form pACYCDuet-Ac9-g119 (20mer ...
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bioRxiv - Biochemistry 2020Quote: The phytochrome from Deinococcus radiodurans strain R1 (DrBphP, gene DR_A0050) in pET21b(+) plasmid (Novagen) was kind gift from Prof ...
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bioRxiv - Neuroscience 2020Quote: ... to study the regulation of the BDNF gene by catecholamines or 0.15% DMSO (Sigma) as a vehicle control in fresh serum-free and antibiotics-free DMEM (DMEM with high glucose ...
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bioRxiv - Cancer Biology 2020Quote: The full-length Aldob and Akt1 genes were inserted into the pET-30a (Novagen) and pGEX4T1 (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: Short hairpin RNAs (shRNA) for target genes and scramble (shCtrl) were purchased from Sigma. pBabe-puro plasmids containing human C/EBPB LAP2 and LIP isoforms were from Addgene (Cat.# 15712 and 15713).
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bioRxiv - Cell Biology 2021Quote: ... the human cTnC gene (TNNC1) within the pET-21a(+) vector was ordered from Novagen and the Phusion site directed mutagenesis kit (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... For brain targeted gene manipulation TTR esiRNA (esiRNA targeting mouse Ttr- EMU030721, Sigma Aldrich)–jetPEI complex was infused into hypothalamus ...
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bioRxiv - Biophysics 2020Quote: ... and the gene product was sub-cloned into the pET16 vector (Novagen, EMD Millipore). For (full length CynR ...
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bioRxiv - Biophysics 2020Quote: ... and the gene product was sub-cloned into the pET16 vector (Novagen, EMD Millipore). For (full length CynR ...
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bioRxiv - Microbiology 2020Quote: ... N gene of SGLV (GenBank accession number MT328777) was cloned into pET-30a (Novagen), and the resulting recombinant plasmid was transformed into Escherichia coli BL21 DE3 strain ...
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bioRxiv - Cell Biology 2022Quote: ... Primers for each gene of interest were ordered from Sigma-Aldrich (Supplementary Table 2), and a separate master mix was prepared for each primer set using the TB Green® Premix Ex Taq II (TLi RNaseH Plus ...
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bioRxiv - Molecular Biology 2019Quote: ... WT and mutated DEF6 plasmids were transfected (Gene-juice Transfection Reagent, Novagen, #70967-3) into COS7 cells and 24h after the transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and (5’-CCCGCGTCTCCCTAGGACCCGA-3’) for Cas9 targeting to the human RAMP2 gene (Sigma, US). All guides were delivered in the LV01 vector (U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP ...
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bioRxiv - Biophysics 2019Quote: ... and the gene product was sub-cloned in the pET20b vector (Novagen, EMD Millipore). Protein derivatives having the cysteine or other point mutations were constructed using QuikChange mutagenesis62 and Megaprimer PCR mutagenesis63 protocols ...
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bioRxiv - Biophysics 2019Quote: ... and the gene product was sub-cloned in the pET20b vector (Novagen, EMD Millipore). Protein derivatives having the cysteine or other point mutations were constructed using QuikChange mutagenesis62 and Megaprimer PCR mutagenesis63 protocols ...
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bioRxiv - Genomics 2021Quote: ... the blasticidin resistance gene was amplified from PSF-CMV-BLAST (Sigma-Aldrich, OGS588-5UG). Amplifications were performed with the Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Developmental Biology 2021Quote: Zinc finger nuclease (ZFN) plasmids targeting the LEPTIN gene were designed by Sigma-Aldrich. Fetal fibroblasts were isolated from 30-day old male pig embryo ...
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bioRxiv - Microbiology 2020Quote: ... To clone the ompR and rstA genes into pBAD24 (55) and pET24 d(+) (Novagen), DNA corresponding to their open reading frames (ORFs ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 ng dsRNA and 1.8 μl X-treme GENE HP DNA transfection reagent (Sigma) were prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... We synthesized the dTrabid gene with codon optimization to Spodoptera frugiperda (Sigma, UK Suppl) and cloned into HindIII and KpnI digested poPINS plasmid ...
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bioRxiv - Neuroscience 2023Quote: Fragments of eaat genes were PCR amplified using a JumpStart Taq polymerase (Sigma-Aldrich) and the primer pairs eaat5b_135_s 5’-GGAGCAGGAAGTCAAGTA-3’ with eaat5b_600_as 5’-GTCCGTCCCATTATCGTC-3’ and eaat7_142_s 5’-GTAATAGCAGGCACAGTGATG-3’ with eaat7_1001_as 5’-CCCAGAGCAGTGATCCAAG-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression was induced by addition of 0.01–0.3 µg/ml doxycycline (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Peroxisome and trafficking pathway gene primers were designed by NCBI and purchased from Sigma. The housekeeping gene GAPDH was used as normalizing control to calculate the fold change.
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bioRxiv - Molecular Biology 2023Quote: ... Gene expression was induced by addition of 0.3 µg/ml of doxycycline (Sigma-Aldrich) for 24 h.
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bioRxiv - Developmental Biology 2023Quote: ... The genes of interest were PCR amplified using JumpStart REDTaq ReadyMix (Sigma-Milipore, P0982) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Plant Biology 2023Quote: ... Synthesized genes were subcloned from the pUC57 cloning vector into the pET-15b (Novagen) expression vector using 1.5 μg of DNA and NdeI and BamHI in 30 μl reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The selected genes were incorporated into a modified pET28a(+) vector (NovagenTM, Sigma-Aldrich Co.) encoding the tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: Synthetic genes codon-optimized for Escherichia coli were cloned into the pET15b vector (Novagen) with an N-terminal hexahistidine (His6)-tag ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...