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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of protein or IP pulldown samples were resolved by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) microporous membrane (Millipore), blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). This was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576 ...
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bioRxiv - Molecular Biology 2020Quote: ... equal masses of protein samples (20 µg) were resolved using SDS PAGE and then transferred to Immobilon-P PVDF membrane (Millipore). Recombinant NOCT (64-431 ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 to 40 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). AGO1 protein was detected using the anti-AGO1 antibody (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). The membranes were then incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... using molecular weight markers (26619, Fermentas) to confirm the expected size of the target proteins and transferred to PVDF membranes (IPVH00010, Millipore). Membranes were washed in TBST buffer (TBS containing 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated on a 10% SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Primary antibody incubation was performed overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 4-20% gradient SDS-PAGE gel (Cosmobio, 414879) and then transferred to PVDF membrane (Millipore, IPVH00010). To detect CAMSAP3 and GAPDH for a loading control ...
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bioRxiv - Genomics 2021Quote: ... Equal amounts of protein were separated by 10% SDS-PAGE gel and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were incubated with RCC2 (CST ...
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bioRxiv - Microbiology 2021Quote: Samples containing equal amounts of protein were separated using sodium dodecyl sulfate (SDS)-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore). After blocking with 5% skim milk in TBST for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... For Western blot analysis the proteins were electrophoretically separated on a 10 % polyacrylamide gel and transferred onto a polyvinyldifluoride membrane (PVDF, Millipore). The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Genetics 2022Quote: ... Equal amounts of protein (40 µg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Immobilon-P membranes (Millipore), as previously described (Nakamori et al. ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Pathology 2022Quote: ... Fifty microgram of protein was separated by SDS electrophoresis using 10% polyacrylamide gel and transferred to an Immobilon PVDF membrane (Millipore). Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817 ...
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bioRxiv - Neuroscience 2022Quote: ... Thirty μg of protein sample was run at 120V to separate proteins based on their molecular weights before electrophoretic transfer onto Polyvinylidene Difluoride (PVDF) transfer membranes (Millipore). PVDF membranes containing separated protein samples were blocked for one hour to prevent non-specific primary antibody binding using a solution of 5% skimmed milk ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked with 5% milk in TBST for 1 h and incubated with antibodies as listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
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bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were resolved on a 10% SDS-PAGE gel and electroblotted onto a PVDF membrane (Sigma Aldrich, Cat # IPVH00005). The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were loaded onto SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of protein samples were loaded onto SDS–PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration of lysates was determined and 70 µg protein was loaded onto a 10% SDS-PAGE and subsequently transferred to a PVDF-FL membrane (Millipore). Membranes were incubated overnight at 4°C in primary antibodies (Flag ...
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bioRxiv - Cell Biology 2024Quote: ... 25-30µg protein was loaded on resolving 12% SDS-PAGE and later transferred to a methanol-activated PVDF membrane (Millipore-# IPVH00010) by using a semi-dry Trans blot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by electrophoresis on precast gradient SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant proteins were resolved on a 10% SDS-PAGE and transferred to a nitrocellulose membrane (0.45 μm, Millipore, USA). Membranes were blocked in blocking buffer (5% nonfat milk ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was transferred to a PVDF membrane 0.2 μm (Amersham) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 min ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, MILLIPORE). Membranes were blocked with 5% nonfat dry milk in PBS with 0.05% Tween20 (PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein lysates were electrophoretically separated on sodium dodecyl sulfate-polyacrylamide gels and electrically transferred to polyvinylidene fluoride membranes (IPVH00010, Millipore). The membranes were blocked in 5% skimmed milk for 1 hour and incubated with the primary antibodies (Table S6 ...
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bioRxiv - Genetics 2023Quote: ... Proteins (5-15 μg) were separated on a 10% NuPAGE Bis-Tris Protein Gel and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked with 5% BSA made up in Tris-buffered saline-Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with a solution containing the membrane pore-forming protein Streptoylsin O (SLO) (Sigma catalog: S5265-25KU) sufficient to permeabilize 90% of HeLa cells ...
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bioRxiv - Molecular Biology 2023Quote: ... the titrations of total protein were separated on 12% SDS-PAGE gel followed by blotting to Immobilon PVDF membrane (Millipore). Membranes were dried and total protein was stained with Sypro Ruby (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... We used direct blue stains of total protein as loading controls by saturating PVDF membrane for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... and splenocytes at 105 cells/well were added to 96-well high-protein-binding Immobilon-P membrane plates (Millipore, UK) that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb ...
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bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Microbiology 2023Quote: Protein samples were resolved by SDS–polyacrylamide gel electrophoresis and transferred to Immobilon®-FL polyvinylidene fluoride membrane (Millipore Sigma) as previously described [76] ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded on a NuPAGE 4-12% Bis-Tris protein gel and transferred onto an Immobilion PVDF membrane (Merk-Millipore) for 1 h at 35 V in a wet transfer apparatus buffered with 12.5 mM Trizma-Base (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 20-40 µg of total protein was separated on 7-10% SDS-PAGE polyacrylamide gels and transferred onto Immobilon PVDF membranes (Millipore). Membranes were blocked using 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...