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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Media was collected and ApoB ELISA Assay (Sigma Aldrich, RAB069) was performed according to protocol ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Biochemistry 2020Quote: ... The cells and media were separated after the incubation and processed for quantification of insulin levels using the RI-13K Rat Insulin RIA Kit (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2020Quote: ... Systemic levels of vascular markers were evaluated at the end of the Fc-GDF15 and vehicle treatment of obese ZSF1 rats by using multiplex MILLIplex kits (Millipore). The rat vascular injury panels included VEGF ...
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bioRxiv - Physiology 2020Quote: ... Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore). Serum NT-proBNP and rat cardiac injury markers (fatty-acid-binding protein 3 [FABP3] and myosin light chain 3 [Myl3] ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The membranes were incubated with primary antibodies overnight at 4⁰C ...
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bioRxiv - Immunology 2021Quote: ... Samples were electrophoresed on 7.5% SDS-polyacrylamide gels and proteins were transferred onto the Immobilon-P membrane (Millipore, New Bedford, MA). Blots were probed with anti-fibrinogen polyclonal antibody (1:100,000 dilution) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... Protein samples (40 μg/lane) were resolved by 10% SDS-PAGE and transferred to Immobilon-P membranes (Millipore, Billierica, MA, USA) by wet transfer (Trans-Blot ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of proteins were further separated using SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Shanghai, China). Membranes were blocked to avoid non-specific bindings in Tris-buffered saline-Tween (TBST ...
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bioRxiv - Immunology 2019Quote: ... equal amounts of protein obtained from the lysed cells were loaded onto 12% SDS-PAGE gels and transferred onto PVDF membranes (Millipore, USA). After blocking with 5% BSA for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Protein from gels were transferred onto PVDF membranes and probed with NSF (SYSY, mouse monoclonal) and beta actin (Sigma, mouse monoclonal) antibodies overnight with shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V with the Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty micrograms of each sample was run on NuPAGE 4–12% Bis-Tris and proteins were electroblotted onto PVDF membranes (Millipore, ISEQ00010), which were further processed as described in the immunoblotting section.
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Equal amount of total protein was loaded and separated on 10% SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membranes (Millipore, IPVH00010). MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2020Quote: ... The same amount of protein was injected into a 10% SDS-PAGE gel for separation and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Germany). Cell membranes were blocked at room temperature for 2 hours with 5% skim milk and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 40μg of total proteins from each sample were separated by 8∼12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA), the membranes were blocked in 5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... of protein were separated by performing SDS-PAGE (10% or 12%) and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPVH00005, Millipore, USA). Membranes were blocked for 1 hours with 5% skim milk at room temperature and then incubated overnight at 4 °C with the primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal volumes of protein samples were separated by 10% SDS/PAGE and electro-transferred to PVDF membranes (Millipore, Billerica, MA, USA). The immunoblots were probed with the indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The crude protein was separated by 12% SDS-PAGE and then transferred to a nitrocellulose filter membrane (Millipore, Billerica, MA, USA) using a Trans-Blot Semi-Dry transfer cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (30 μg) were separated in a 10% SDS-polyacrylamide gel and then transferred to PVDF membranes (Millipore, USA). The membranes were blocked in 5% w/v bovine serum albumin before incubation with the primary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein (40 μg) were resolved by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore, MA, USA). Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Systems Biology 2019Quote: ... The extracted proteins were analyzed by 5-20% gradient SDS-PAGE and transferred onto Immobilon transfer membranes (Millipore, Billerica, MA, USA) for immunoblotting analyses ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on 10% SDS-PAGE gels and electrotransferred onto Immobilon-polyvinylidene difluoride membranes (Millipore Sigma, Burlington, MA). After blocking with 5% non-fat milk ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Aliquots (40 μg) of protein samples were subjected to 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Bedford, MA, USA). After blocking with 5% nonfat milk (dissolved in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins extracted from the brain tissues were separated by SDS-PAGE and electro-transferred to a PVDF membrane (Millipore, Bedford, MA). The membrane was blocked with 2% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Fifty micrograms of protein was fractionated via 10% SDS-PAGE and transferred to Immobilon Transfer Membranes (Millipore Corporation, Billerica, MA, USA). Primary antibodies used in this study included those against β-actin (GTX109639 ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were fractionated by 10-15% SDS gels and electro-transferred to polyvinylidene difluoride transfer membranes (#IPVH00010, Millipore, Billerica, MA, USA). The membrane was incubated overnight with primary antibodies and then with secondary antibodies conjugated with horseradish peroxidase (#7074S and #7076S ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
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bioRxiv - Neuroscience 2022Quote: ... 600 μg protein equivalents of exosomes pelleted by ultracentrifugation were resuspended in 500 μl Diluent-C for membrane labeling (Sigma CGLDIL) and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET) ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples dissolved in Laemmli buffer were separated on SDS-PAGE gel and transferred to Immobilon-P PVDF membrane (Millipore, IPVH00010). Transferred protein samples were detected using following primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of proteins (20 μg/lane) were loaded onto 8% or 10% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, USA). The membranes were then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: The nuclear protein extracts from the TNF-stimulated A549 cells were subjected to SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, USA) using the transfer buffer (192 mM glycine ...