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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2019Quote: ... Extracted proteins were then separated via SDS-PAGE and then transferred to a 0.45 μM polyvinylidene fluoride (PVDF) membrane (Millipore) on a TRANS-BLOT SD apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked and incubated with primary antibodies against FGF2 (36769 ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins samples (20 μg/lane) were separated by SDS–PAGE and transferred onto PVDF membranes (0.45μm, Millipore, USA) using a transfer unit (Bio–RAD ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on 12% SDS–PAGE and proteins were transferred to methanol-activated polyvinylidene fluoride (PVDF) membrane (Millipore) using anode buffer (25 mM Tris HCl pH 10.4 ...
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bioRxiv - Biochemistry 2019Quote: The proteins in an SDS-polyacrylamide gel were transferred onto an Immobilon®-P PVDF transfer membrane (Millipore Sigma) by electroblotting at 250mA for two hours in ice cold transfer buffer (25mM Tris ...
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bioRxiv - Pathology 2020Quote: ... cell lysates containing equal protein amounts were separated by SDS-PAGE and transferred to PVDF membranes (Cat.#ISEQ00010, Millipore). Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein samples were separated by 10% SDS-PAGE gel and then electrically transferred to 0.45μm PVDF membranes (Millipore, IPVH00010). Membranes were then blocked with 5% non-fat milk in TBST for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 5μg of total cell lysate proteins were resolved on 4-12% SDS-PAGE and transferred onto 0.45 μm polyvinyl difluoride membrane (Millipore) as described above under western blotting of EVs.
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...
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bioRxiv - Microbiology 2021Quote: ... Extracellular proteins (10 μg/mL; totally 700 mL) were filtered through a 0.22-μm nitrocellulose membrane (47-mm diameter; Millipore) and concentrated using the Amicon Ultra Centrifugal Filters to 7 mL (cut-off of 30 kDa) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Genetics 2020Quote: ... Protein samples were subjected to SDS-PAGE and wet-transferred to a 0.2 μM Immobilon-PSQ PVDF membrane (Millipore) at 200 mA for 90 minutes ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed in TBS-T and proteins were visualized using a chemiluminescent substrate (Sigma Aldrich, Oakville, ON, Canada).
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bioRxiv - Biophysics 2022Quote: ... Purified Vnucfree solubilized in 0.03% n-DDM was mixed with the lipid stock and membrane scaffold protein MSP1E3D1 (Sigma) at a specific molar ratio of enzyme ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by electrophoresis through 12% polyacrylamide gels containing SDS (0.1%) and transferred to a PVDF membrane (Millipore). The membrane was blocked with 2% (w/v ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Plant Biology 2022Quote: ... 40 μg total proteins were loaded into 10% acrylamide gels and transferred to Immobilon-P membrane (Millipore, Cat. IPVH00010). The antibody were diluted as follows ...
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bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
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bioRxiv - Immunology 2022Quote: ... Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore, Burlington, MA). Membrane was then blocked by incubating in 5% skim milk/TBS for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins (20-40 μg) were subjected to 10% SDS-PAGE and electrotransferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore). After incubation in a blocking buffer (5% bovine serum albumin/TBST ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were run on EZ Protein any KD PAGE (LIFE-iLAB) and then transferred to 0.22um PVDF membranes (Millipore). Membranes were blocked using 5% non-fat milk in PBS-Tween20 0.1% for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated on 6-10% SDS-PAGE gels and transferred to methanol pre-activated PVDF membranes (Millipore, #IPVH00010). For MYCBP2 blots ...
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bioRxiv - Biochemistry 2023Quote: Equal amounts of protein from cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore). HeLa GFP and HeLa TRAP1-GFP cells were treated with either 2 μg/mL of harringtonine and 100 μg/mL of emetine for 15 min at 37°C to inhibit cytosolic translation or 30 μM linezolide or 200 ng/mL chloramphenicol for 1 hr at 37°C to inhibit mitochondrial translation ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a PROTRAN 0.2 µm nitrocellulose membrane (Cytiva) and stained with Ponceau S (Sigma-Aldrich). Membranes were incubated in 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of total proteins (20μg) were separated by SDS-PAGE and transferred to a 0.45-mm Immobilon-P PVDF Membrane (Millipore, Merck) and the membrane was incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Amersham Protran, GE10600001, Sigma Aldrich) by the wet transfer method ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane and blocked with PBS + 0.1% Tween 20 + 5% Milk powder (Sigma) and incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and electrophoretically blotted onto microporous polyvinylidene fluoride membranes (Millipore) under a constant current ...
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bioRxiv - Cancer Biology 2023Quote: ... 30–50 μg of protein was fractionated by 10% SDS–PAGE and blotted onto an Immobilon PVDF membrane (Millipore). Non-specific binding sites were blocked by incubation with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein per sample were subjected to SDS–PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). For immunoprecipitation of FLAG-tagged proteins ...
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated by 7.5% SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, Billerica, MA, USA), as previously described (Towbin et al. ...
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bioRxiv - Neuroscience 2024Quote: Equal amounts of proteins from total cell lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The blots were blocked with 5% w/v BSA and 0.05% NaN3 in TBST and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were labelled with the appropriate set of antibodies: primary rat anti-HA (1:500, Sigma), mouse anti-Myc (1:1000 ...
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bioRxiv - Plant Biology 2019Quote: ... and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P, 1:10,000, Sigma). HRP activity was detected by the Supersignal Western Detection Reagents (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were used at the following concentrations: rat anti-HA 3F10 at 1:400 (Sigma-Aldrich), mouse anti-Myc 9B11 at 1:2000-1:3000 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with primary antibody rat anti-somatostatin (1:250, Millipore cat. No. MAB354, RRID:AB_2255365) at RT for 24 hours and then 4°C for 42 hours ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253 ...
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bioRxiv - Bioengineering 2020Quote: ... P0-1 SD rat brains were removed and rinsed in phosphate buffer saline (PBS, Sigma-Aldrich). After careful removal of the meninges and brains cortices ...