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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
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bioRxiv - Biochemistry 2024Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Rubisco was labelled using an Atto594 protein labelling kit (Sigma-Aldrich), according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of protein lysate were resolved by SDS-PAGE and subsequently transferred onto polyvinylidene difluoride Immobilon-FL membrane (Millipore). Following transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were separated in sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Cancer Biology 2019Quote: ... Then 10 μg of nuclear protein extracts were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore). After blocking with QuickBlockTM Blocking Buffer for Western Blot (Beyotime) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were transferred to a PVDF membrane followed by incubation with Anti-Cas9 antibody conjugated to FITC (Sigma Aldrich). Anti-mouse secondary antibody conjugated to Alkaline phosphatase was used to develop the blot with NBT-BCIP (nitro-blue tetrazolium and 5-bromo-4-chloro-3’-indolyphosphate).
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred onto polyvinylidene fluoride membrane (PVDF, Hybond-P, Amersham) in transfer buffer containing 12 mM Tris (Sigma), 96 mM glycine (BDH ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were separated by SDS-PAGE (10% acrylamide gel) and transferred to a nitrocellulose transfer membrane (Immunobilon – EMD Millipore) using a semi-dry transfer set up ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein extracts (40 μg) were separated by electrophoresis on 10% SDS polyacrylamide gel and transferred onto polyvinylidene difluoride membranes (Millipore). Membranes were blocked in 5% nonfat milk in TN buffer (50 mmol/L Tris-HCl pH7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Equal protein concentrations were loaded onto 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore Corporation, Billerica, Mass.). Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... Western immunoblotting was performed by incubating the membrane with Streptavidin HRP-linked protein in PBS-BSA buffer and developing with TMB (Sigma).
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bioRxiv - Immunology 2021Quote: ... A total of 105 cells or 20 mg protein was resolved by SDS-PAGE and transferred to Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk in TBS-T (25 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of protein were loaded and separated in 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membranes (Millipore). Membranes were blocked for 1 hour at room temperature with Intercept (TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Bioengineering 2020Quote: ... For western blotting 40 μg of protein samples were subjected to 10% in house SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 3% BSA in TBS containing 0.01 % Tween-20 for 1 h at room temperature and then incubated with specific primary antibodies at 4 °C overnight in the blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein samples were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, MA, USA). The membranes were treated with 1% blocking solution in TBS for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 25 µg of the protein was loaded in 4-12% Bis-Tris gradient polyacrylamide gel and transferred onto PVDF membrane (Millipore). Membranes were blocked in PBS with 0.1% Tween 20 and probed with respective primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (11 µg) were run on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore). Membranes incubated with the primary antibodies against claudin-5 (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein samples (30 μg) were resolved in 12%-SDS-PAGE and transferred to ImmobilonTM PVDF membranes (Millipore, Burlington, MA) in a semi-dry electroblotting apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... 20-30μg of protein extract was separated on a 4-20% precast polyacrylamide gel and transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% nonfat milk in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Eight micrograms of protein per lane were separated by 12% SDS polyacrylamide gel electrophoresis and blotted onto Immobilon-P nylon membranes (Millipore). After transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Biophysics 2021Quote: 5−10 μg of proteins were separated on 10% SDS-PAGE and were consecutively electro blotted onto 0.45 µm nitrocellulose membrane (Millipore, USA). The membrane was blocked with 5% non-fat skim milk in PBS-0.1% Tween 20 for 2 hours at room temperature ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: Equal amount of protein lysates was loaded and resolved on SDS-PAGE gel and transferred onto an PVDF membrane (Millipore). Blots were blocked with 5% BSA for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of proteins were separated by electrophoresis in an 10% SDS polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (PVDF, Millipore). The membranes were blocked with 5% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved by SDS PAGE and transferred to an Immobilon P polyvinylidene fluoride or Immobilon-FL membrane (EMD Millipore)64 ...
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bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... and 50μg of protein was resolved on 10–12% SDS-PAGE followed by transferring to nitrocellulose or PVDF membranes (EMD-Millipore). For immunoprecipitation ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins (30 µg) from brain cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp.). Membranes were incubated with anti-TSP1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were separated by SDS-PAGE under reducing conditions and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA). Antibody incubations were performed in 3% BSA in TBS buffer using the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 200µl of total protein was further fractionated into soluble and aggregated forms using membrane based centrifugal filter unit (Millipore MRCF0R100) 500X g for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing equal amounts of protein were separated by SDS-PAGE (4% stacking and 12% resolving gel) and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 5% skim milk (for detection of E ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10-30 μg total cellular protein were resolved on SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Bound antibodies were detected with IRDye infrared secondary antibodies using the Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein samples were separated by sodium dodecyl sulfate (SDS)-PAGE and transferred onto a nitrocellulose membrane (Millipore, Temecula, CA, USA). After blocking with 5% non-fat powdered milk ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was transferred to a PVDF membrane (0.2 micron, Immobilon) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 mins ...