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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was normalized to the total protein for each sample determined after transfer to the membrane using Ponceau S solution (Sigma).
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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bioRxiv - Neuroscience 2023Quote: ... Normalized total proteins were analyzed by electrophoresis in 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were heat-denatured for 5 min at 100°C and run on a 4-20% Criterion Tris-HCl Protein gel and transferred to PVDF membrane (Millipore). Membranes with muscle tissue homogenates were blocked in 5% non-fat dry milk in TBS/0.2% Triton X-100 and incubated overnight in Tau12 (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to PVDF membrane (Millipore). The membrane was blocked with 5 % skim milk in tris-buffered saline with 0.1% Tween20 (TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically transferred to nitrocellulose membranes (Millipore), and blocked in 5% nonfat dry milk ...
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bioRxiv - Cell Biology 2023Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, MILLIPORE). Membranes were blocked with 5% nonfat dry milk in PBS with 0.05% Tween20 (PBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... About 30 µg of total cell protein was separated on 8% SDS-PAGE and blotted onto Immobilon-P PVDF membrane (Millipore). The membrane was blocked in 0.2% I-Block (Tropix) ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (EMD Millipore, IPVH00010), then blocked in Tween 0.1% Tris-buffered saline containing 5% bovine serum albumin (Tocris 5217 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immobilon-P, Millipore, Bedford, MA). Membranes were blocked with PBS plus 0.1% Tween 20 (PBST ...
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bioRxiv - Cell Biology 2024Quote: ... we used direct blue stains of total protein by saturating PVDF membranes for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5–40 μg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). GFP was detected using the anti-GFP antibody (Mouse monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Glial Fibrillary Acidic Protein (1/1000, Millipore, MAB360). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced using 1 mM dithiothreitol (Sigma-Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-ACTB reference protein (Sigma Aldrich, A2066, 1:10000) antibody was also used ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-microtubule associated protein 2 (MAP2, 1:200, Millipore), mouse monoclonal anti-ankyrinG (AnkG ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM NaVO3) with protein inhibitor cocktail (Sigma®) followed by total protein quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Cell Biology 2024Quote: N-terminally His6-Smt3-tagged proteins (pRSFDuet-1, Novagen) were expressed in Rosetta (DE3 ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated with blocking solution (1% cold water fish gelatin; Sigma #G7765), primary antibodies (1:500 Broad Core ...
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bioRxiv - Immunology 2022Quote: ... The membrane was incubated with antibodies against pan-talin (1:1000, Sigma-Aldrich) or actin (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were incubated for 1 hour with primary antibody M2 α-FLAG (Sigma) at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with an anti-Hb antibody (1:5.000) (Sigma-Aldrich). The antigen-antibody complex was revealed by chemiluminescence.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were incubated with anti-FLAG antibody (1:400, Sigma Aldrich, Cat# F3165) overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked for 1 hour at RT in a phosphoprotein blocker (Millipore) and probed for total (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:2000; Sigma-Aldrich) and were developed by using an enhanced chemiluminescence detection kit according to the manufacturer’s instructions (Supersignal West Dura or Femto ...
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bioRxiv - Microbiology 2022Quote: ... whilst 1 μM of the membrane potential-sensitive dye DiSC3(5) (Sigma-Aldrich) was added 5 min prior to imaging ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were re-probed with β-actin (1:5000; Sigma-Aldrich, Cat. #A2228) as a loading control ...
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bioRxiv - Developmental Biology 2020Quote: ... Membrane was incubated in TBST + 1:1000 Anti-FLAG-M2-HRP (Sigma, #8592) at 4°C overnight while rolling ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed with the following primary antibodies: APP 22C11 (1/1000, Millipore), APP-CTF (1/1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h with 5% milk (Sigma-Aldrich, Dorset, UK) (in tris-buffered saline and 1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with primary antibodies [mouse α-FLAG M2 (1:1000; Sigma); rabbit α-ICDH (1:1,000 ...
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bioRxiv - Bioengineering 2019Quote: ... Membranes were probed for α-tubulin (Sigma-Aldrich, Cat. No. T6074; 1:4000) and iNOS (1:1000) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with rabbit anti-human APOL1 (1 μg/mL) (Sigma-Aldrich) and mouse anti-human tubulin (1 μg/mL ...