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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: The nuclear protein extracts from the TNF-stimulated A549 cells were subjected to SDS-PAGE and transferred to a nitrocellulose membrane (Millipore, USA) using the transfer buffer (192 mM glycine ...
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bioRxiv - Developmental Biology 2023Quote: ... All proteins were separated in 5-22% gradient SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, Billerica, MA) using a semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... each sample with equal amounts of protein was subjected to SDS–PAGE and transferred to a PVDF membrane (Millipore, Burlington, MA). The membranes were incubated with the primary antibody at 4 ℃ overnight ...
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bioRxiv - Plant Biology 2023Quote: ... Forty µg of total protein from each sample was resolved in 12% SDS gel and blotted onto a PVDF membrane (Millipore, USA). The blot was blocked with 5% non-fat milk in TBST buffer (50 mM Tris–HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was quantified by Bradford assay54 and 20 μg of protein of each cell line were separated by SDS-PAGE and electrotransferred to PVDF membranes (Millipore Sigma). The primary antibodies anti-ZIP11 and anti-GAPDH as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of denatured protein homogenate were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to a polyvinylidene difluoride membrane (Millipore, USA). After blocking at room temperature for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal amount of protein (10μg) per sample was separated on 10% SDS polyacrylamide gels and then transferred onto PVDF membranes (Millipore, MA, USA). The membrane was blocked with 4% non-fat milk in TBST buffer at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated using a 10% sodium dodecyl sulfate polyacrylamide gel and transferred to immobilon polyvinylidene difluoride membranes (EMD Millipore). After blocking for one hour at room temperature in odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P, Millipore, IPVH00010). After blocking the membranes in 5% BSA in TBST (1× TBS (Cell Signalling) ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Immobilion-P, Millipore, Schwalbach, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the phage suspensions were further filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts), diluted and plated as indicated in the previous paragraph to verify the uniformity of the plaques ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of proteins were loaded onto polyacrylamide gels (8-12%) under reducing conditions and transferred to Immobilon-P membranes (EMD Millipore). After blocking with 5% BSA (wt/vol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein 20 ug were separated by SDS-PAGE and further transferred onto 0.2 um PVDF membrane (Millipore, Cat. No. IPVH00010) pre-soaked with ethanol ...
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bioRxiv - Microbiology 2024Quote: ... were electrophoresed on 10% sodium dodecyl sulphate- polyacrylamide gel and the separated proteins were transferred onto the polyvinylidene difluoride membranes (Millipore, USA) The membranes were blocked with Tris-buffered saline with Tween (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μg of protein lysate was electrophoresed on 12% SDS-polyacrylamide gels and transferred to PVDF membranes (EMD Millipore, Burlington, MA). After blocking with 5% milk ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 30 μg of protein was separated on a 12% polyacrylamide gel and transferred onto a PVDF membrane (Immobilon-P, Millipore, IPVH00010) in a 20 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were equilibrated in pre-chilled transfer buffer with 15% methanol and then protein was transferred to a 0.45 µm nitrocellulose membrane (Sigma catalog# 10600002) via wet electroblotting on ice for 1 h at 300 mV ...
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bioRxiv - Microbiology 2024Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Microbiology 2019Quote: ... the PKH67 green fluorescent cell linker kit for general cell membrane labeling (Sigma Aldrich, Munich, Germany) was used and the protocol was adapted to O ...
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bioRxiv - Neuroscience 2024Quote: ... PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (Sigma-Aldrich Cat no: PKH67GL).
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bioRxiv - Developmental Biology 2023Quote: ... RESCs were derived as reported previously.40 Rat blastocysts were gently flushed out from the E4.5 timed-pregnant rats’ uteruses using M2 medium (Millipore). Zona pellucida was removed with acid Tyrodes solution (Sigma) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Proteins were visualized after staining with ProteoSilver Silver Stain Kit (Sigma-Aldrich) (S.Fig.1 ...
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bioRxiv - Neuroscience 2021Quote: ... we used the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) following the manual with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich), using BSA as a standard and aliquots maintained at −80°C until required ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using the bicinchoninic acid assay (BCA) kit (Sigma).
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma Alrich). Protein lysates (50 μg ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein activity was measured by an ATPase activity kit (Sigma-Aldrich) as described by the manufacturer.
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bioRxiv - Pathology 2020Quote: Dissolve proteins and FITCs in FluoroTag™ FITC Conjugation Kit(Sigma-Aldrich)kit buffer ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Immunology 2021Quote: RIP was performed by Magna RIP RNA binding protein immunoprecipitation kit (Millipore). A549 cells were lysed into RIP lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a BCA assay kit (#QPBCA, Sigma-Aldrich). Protein samples were mixed with DTT (#D9779-10G ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were incubated overnight at 4°C in primary antibody diluted in 5% BSA/PBS using the following concentrations: 1:500 rabbit-α- PfACP48 (kind gift from S. Prigge) and 1:100 rat-α-HA 3F10 (Sigma 11867423001). Coverslips were washed 3 times in PBS ...
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bioRxiv - Synthetic Biology 2022Quote: Male and female rats were immunized in the flanks with 200 μl of a 1:1 emulsion of ovalbumin (Sigma, St. Louis, MO) in complete Freund’s adjuvant (Difco/Becton Dickinson ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was probed for HA-epitope (rabbit anti-HA, Cell Signaling #3724, 1:1000; or rat anti-HA, Sigma #11867423001, 1:1000), or PSD95 (mouse anti-PSD95 ...