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Citations for Millipore Sigma :
2001 - 2050 of 2656 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Aqueous solutions of chlorhexidine-gluconate are most stable within the pH range of 5-8 (https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c9394pis.pdf). The Mueller-Hinton broth adjusted to pH=5 did not affect the visible growth of E ...
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bioRxiv - Genomics 2021Quote: ... and the lentiviral library (MOI = 0.5) was transduced into the iPSCs with 8 µg/mL Polybrene (Millipore, TR-1003-G) and spun at 1000 RCF at 37°C for 90 min ...
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bioRxiv - Genomics 2021Quote: ... fluorescent E-barcodes were defined by patterning the SU-8 structure doped by adding 0.004 wt‰ of Rhodamine 6G powder (Sigma-Aldrich) into SU-8 precursor.
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bioRxiv - Genetics 2021Quote: ... 0.2×106 mESCs were seeded per 12-well and transduced the next day with 500 µl of viral supernatant and 8 ng/µl polybrene (Sigma). Antibiotic selection was started two days after transduction and kept for at least 3 passages.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 7-dichloro-8-hydroxyquinoline (CLCQ; D64600-Lot#STBH7389) and Zinc Chloride (ZnCl2; 208086-Lot#MKCL1763) were purchased from Sigma Aldrich. We prepared 10mM stocks solutions of the inhibitors in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... After a release for 8 h in fresh media they were incubated with 50 ng/mL nocodazole (Sigma-Aldrich, M1404) for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1 M PBS and then developed with a 8 min incubation in DAB-Nickel solution: 10 mg 3,3′-diaminobenzidine (DAB) tablet (Sigma, DF905), 0.5 ml of a 2 % aqueous nickel sulfate solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were counterstained with hematoxylin (for CD45, MPO, IBA1, CD3, Ki67, 8-OHdG, and pH2AX) or Hoechst 33258 (Sigma-Aldrich) for HMGB1 ...
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bioRxiv - Systems Biology 2021Quote: ... the membrane is blocked using 5% milk in TBS-T buffer (Tris-Buffered Saline pH 7.6 [TBS {20 mM Tris pH 8, 150 mM NaCl, Hydrochloric acid [Sigma Aldrich, 320331]}] with 0.1% Tween-20 [Fischer Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Sections (8-10 μm thick) were obtained using a Leica RM2045 microtome and stained with 0.1 % Ruthenium Red (Sigma-Aldrich) in PBS for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Interleukin 3-dependent hematopoietic pro-B cell line Ba/F3 (57) were transduced with the retroviral supernatant supplemented with 8 μg/ml of polybrene (Sigma) and IL-3 ...
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bioRxiv - Cancer Biology 2020Quote: ... 17 β-estradiol was maintained in 200-proof ethanol at 2.7 mg/ml stock solution and added to drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). Lapatinib (SelleckChem ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 or MDA-MB-231 cells were transduced with concentrated viruses in the presence of 8 ug/ml of polybrene (Sigma) overnight or spin transduced at 500g at 32 °C for 90 min followed by incubation at 37 °C for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... confluent EnSC monolayers were down-regulated in phenol-free DMEM/F□12 media containing 2% DCC□FBS and decidualized with 10 μM medroxyprogesterone acetate (MPA) and 0.5 mM 8 bromo cAMP (C+M treatment) (Sigma Aldrich). To study the effect of exogenous HA ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 μL concentrated lentivirus encoding GC-C linked to a GFP reporter was added to a total of 1 mL growth medium containing 8 μM polybrene (Sigma). 24 hours post transduction ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 or HepAD38 cells were transduced by the harvested viral supernatants in the presence of 8 μg/ml polybrene (Sigma), followed by selection with 2 μg/ml puromycin (Clontech) ...
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bioRxiv - Immunology 2020Quote: ... 8-20 weeks after reconstitution chimeric mice were injected intraperitoneally for 3 d with 2 mg/day of tamoxifen (Sigma) re-suspended at 20 mg/ml in corn oil (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 μg/ml of streptomycin (Sigma), and 2 mM L-Glutamine (PAA) ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3+ T and Jurkat cells were cultured with lentivirus solution for 24 h in the presence of 8 μg/mL Polybrene (Sigma).
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bioRxiv - Immunology 2021Quote: ... Fractions containing the proteins were pooled and concentrated to ~ 8 mg/mL each with 30 kDa cutoff ultrafiltration units (Millipore). Time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Immunology 2021Quote: ... 8 weeks old mice were injected intraperitoneally with the procarcinogen azoxymethane (AOM) (10 mg/kg of body weight, Sigma-Aldrich). After 1 week ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded at 200,000 cells/well of 6 well plates and transduced with lentiviral particles at final MOI of 0.5 with 8 µg/ml polybrene (Sigma-Aldrich). Transduced cells were selected in 10 µg/ml puromycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: NK92 cells were transduced with each pCDH lentivirus (multiplicity of infection is 30) in the presence of 8 μg/mL polybrene (Sigma). Then ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins from whole cell lysates (20–60 µg) were separated on 8-10-12% polyacrylamide gels and transferred to Immobilon-FL membranes (Millipore). Primary and secondary antibodies were diluted in blocking buffer (Millipore ...
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bioRxiv - Genomics 2021Quote: ... Samples were diluted 1:100–1:300 in TE with DTT buffer (10 mM Tris pH 8, 1mM EDTA, 200 mM DTT, Sigma) and were stained with 130 nM YOYO-1 (Invitrogen ...
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Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... sections were stained for 8 min in acidic haemalum staining solution (Waldeck) and for 2,5 min in eosin staining solution (Sigma-Aldrich) at room temperature using a Tissue-Tek Prisma Plus slide stainer (Sakura).
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bioRxiv - Neuroscience 2022Quote: Deeply anesthetized 8-9 week old male and female ICR mice were injected retro-orbitally with 0.4 ml of HRP (Sigma Aldrich, HRP type II ...
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bioRxiv - Neuroscience 2022Quote: ... meninges were removed and individual cortices were transferred to HBSS containing 8 µl Trypsin (25g/L in 0.9 % NaCl) (Sigma, T4549), incubated for 20 min at 37 °C with agitation ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown in 15cm dishes and transfected with pcDNA3.1-BirA-Myc (2 μg + 8 μg pcDNA3 as a carrier plasmid) or pcDNA3.1-BirA-Myc-ACLY (10 μg) using X-tremeGENE 9 (Sigma, 6365779001). At the time of transfection ...
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bioRxiv - Biochemistry 2022Quote: ... The IMAC pool was diluted 1:4 in 50 mM Tris HCl pH 8 and applied to 4 mL DEAE-Sepharose (Sigma). After washing with 20 mM Tris HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed five times with 200 μL of streptavidin wash buffer (50 mM Tris-HCl and 8 M urea, pH 7.4, both Sigma-Aldrich), then equilibrated by washing three times with 200 μL of 1x standard ligation buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μg of dsRNAs were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) every 3 days four times ...
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bioRxiv - Biochemistry 2022Quote: ... The elution was separated by 8% Nu-PAGE and analyzed by CBB staining or immunoblotting using an anti-Flag antibody (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... virus-containing culture supernatant was harvested and added to the growth medium of cells in combination with 2.5-8 μg/ml polybrene (EMD Millipore). Populations of each cell line were selected by FACS or antibiotics (Blasticidin ...
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bioRxiv - Cancer Biology 2022Quote: Cells were incubated with lentiviral supernatant for 24 h in the presence of 8 μg/μl of polybrene (Sigma-Aldrich) at a multiplicity of infection >5 unless otherwise stated.
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bioRxiv - Cell Biology 2022Quote: ... with N2 (Thermo, 17502048), B27 (Thermo, 12587010), Glutamax (Thermo, 350050061) and freshly supplemented with 8 μM of CHIR (Sigma, SML1046) and 25 ng/ml of BMP4 (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of the filtered viral particles and 8 µg/ml of hexadimethrine bromide (polybrene) (Cat. Nº: Sigma-Aldrich. 107689). The following day media was change with fresh media ...
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bioRxiv - Cell Biology 2022Quote: ... to sort each well containing 80 nuclei per well into new four 96-well plates (in total of 384 wells) containing 8 μL primer dilution buffers (15 pmol primer i7, 15 pmol primer i5, 200 ng BSA (Sigma), 0.027% SDS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated by 8–15% sodium dodecyl sulfate-polyacrylamide gels and transferred to a PVDF membrane (IPVH00010 Millipore Immobilon). After transfer ...
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bioRxiv - Genetics 2022Quote: ... 8 to10 weeks of age female mice were administered with isoproterenol (30 mg per kg body weight per day, Sigma) for 21 days using ALZET osmotic minipumps ...
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bioRxiv - Genetics 2022Quote: ... 0.2 × 106 mESCs were seeded per 12-well and transduced the next day with 50 μl of concentrated viral supernatant and 8 ng/μl polybrene (Sigma). Antibiotic selection was started 2 days after transduction and kept until all cells in the non-transduced control were dead (typically within 1-2 passages).
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged at 12,000 RPM for 8 min at 37°C and cleared by passing through 0.22 μm Spin-X tube filters (Sigma–Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Adult mosquitoes of both species were provided with a sugar solution containing 8% fructose (Emprove® Chemicals, Sigma, MO, USA) ad libitum.
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bioRxiv - Molecular Biology 2022Quote: ... cells were seeded into 12-well tissue culture trays at a density of 2×105 cells per well and pretreated with 10-8 M phorbol 12-myristate 13-acetate (PMA) (Sigma) for 72 h ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... were first mixed on a magnetic stirrer for 30 min and then 8 drops of DMP-30 accelerator (#45348 Sigma) were added (Knott et al. ...