Labshake search
Citations for Millipore Sigma :
1901 - 1950 of 2487 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
-
bioRxiv - Microbiology 2021Quote: ... HepG2 or HepAD38 cells were transduced by the harvested viral supernatants in the presence of 8 μg/ml polybrene (Sigma), followed by selection with 2 μg/ml puromycin (Clontech) ...
-
bioRxiv - Immunology 2020Quote: ... 8-20 weeks after reconstitution chimeric mice were injected intraperitoneally for 3 d with 2 mg/day of tamoxifen (Sigma) re-suspended at 20 mg/ml in corn oil (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 μg/ml of streptomycin (Sigma), and 2 mM L-Glutamine (PAA) ...
-
bioRxiv - Molecular Biology 2020Quote: ... CD3+ T and Jurkat cells were cultured with lentivirus solution for 24 h in the presence of 8 μg/mL Polybrene (Sigma).
-
bioRxiv - Immunology 2021Quote: ... Fractions containing the proteins were pooled and concentrated to ~ 8 mg/mL each with 30 kDa cutoff ultrafiltration units (Millipore). Time-resolved fluorescence resonance energy transfer (TR-FRET ...
-
bioRxiv - Immunology 2021Quote: ... 8 weeks old mice were injected intraperitoneally with the procarcinogen azoxymethane (AOM) (10 mg/kg of body weight, Sigma-Aldrich). After 1 week ...
-
bioRxiv - Cell Biology 2020Quote: ... were seeded at 200,000 cells/well of 6 well plates and transduced with lentiviral particles at final MOI of 0.5 with 8 µg/ml polybrene (Sigma-Aldrich). Transduced cells were selected in 10 µg/ml puromycin (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: NK92 cells were transduced with each pCDH lentivirus (multiplicity of infection is 30) in the presence of 8 μg/mL polybrene (Sigma). Then ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins from whole cell lysates (20–60 µg) were separated on 8-10-12% polyacrylamide gels and transferred to Immobilon-FL membranes (Millipore). Primary and secondary antibodies were diluted in blocking buffer (Millipore ...
-
bioRxiv - Genomics 2021Quote: ... Samples were diluted 1:100–1:300 in TE with DTT buffer (10 mM Tris pH 8, 1mM EDTA, 200 mM DTT, Sigma) and were stained with 130 nM YOYO-1 (Invitrogen ...
-
Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinomabioRxiv - Cancer Biology 2020Quote: ... sections were stained for 8 min in acidic haemalum staining solution (Waldeck) and for 2,5 min in eosin staining solution (Sigma-Aldrich) at room temperature using a Tissue-Tek Prisma Plus slide stainer (Sakura).
-
bioRxiv - Neuroscience 2022Quote: Deeply anesthetized 8-9 week old male and female ICR mice were injected retro-orbitally with 0.4 ml of HRP (Sigma Aldrich, HRP type II ...
-
bioRxiv - Neuroscience 2022Quote: ... meninges were removed and individual cortices were transferred to HBSS containing 8 µl Trypsin (25g/L in 0.9 % NaCl) (Sigma, T4549), incubated for 20 min at 37 °C with agitation ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were grown in 15cm dishes and transfected with pcDNA3.1-BirA-Myc (2 μg + 8 μg pcDNA3 as a carrier plasmid) or pcDNA3.1-BirA-Myc-ACLY (10 μg) using X-tremeGENE 9 (Sigma, 6365779001). At the time of transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... The IMAC pool was diluted 1:4 in 50 mM Tris HCl pH 8 and applied to 4 mL DEAE-Sepharose (Sigma). After washing with 20 mM Tris HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... The beads were washed five times with 200 μL of streptavidin wash buffer (50 mM Tris-HCl and 8 M urea, pH 7.4, both Sigma-Aldrich), then equilibrated by washing three times with 200 μL of 1x standard ligation buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... 5 μg of dsRNAs were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) every 3 days four times ...
-
bioRxiv - Biochemistry 2022Quote: ... The elution was separated by 8% Nu-PAGE and analyzed by CBB staining or immunoblotting using an anti-Flag antibody (Sigma).
-
bioRxiv - Cancer Biology 2022Quote: ... virus-containing culture supernatant was harvested and added to the growth medium of cells in combination with 2.5-8 μg/ml polybrene (EMD Millipore). Populations of each cell line were selected by FACS or antibiotics (Blasticidin ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were incubated with lentiviral supernatant for 24 h in the presence of 8 μg/μl of polybrene (Sigma-Aldrich) at a multiplicity of infection >5 unless otherwise stated.
-
bioRxiv - Cell Biology 2022Quote: ... with N2 (Thermo, 17502048), B27 (Thermo, 12587010), Glutamax (Thermo, 350050061) and freshly supplemented with 8 μM of CHIR (Sigma, SML1046) and 25 ng/ml of BMP4 (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 ml of the filtered viral particles and 8 µg/ml of hexadimethrine bromide (polybrene) (Cat. Nº: Sigma-Aldrich. 107689). The following day media was change with fresh media ...
-
bioRxiv - Cell Biology 2022Quote: ... to sort each well containing 80 nuclei per well into new four 96-well plates (in total of 384 wells) containing 8 μL primer dilution buffers (15 pmol primer i7, 15 pmol primer i5, 200 ng BSA (Sigma), 0.027% SDS ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were separated by 8–15% sodium dodecyl sulfate-polyacrylamide gels and transferred to a PVDF membrane (IPVH00010 Millipore Immobilon). After transfer ...
-
bioRxiv - Genetics 2022Quote: ... 8 to10 weeks of age female mice were administered with isoproterenol (30 mg per kg body weight per day, Sigma) for 21 days using ALZET osmotic minipumps ...
-
bioRxiv - Genetics 2022Quote: ... 0.2 × 106 mESCs were seeded per 12-well and transduced the next day with 50 μl of concentrated viral supernatant and 8 ng/μl polybrene (Sigma). Antibiotic selection was started 2 days after transduction and kept until all cells in the non-transduced control were dead (typically within 1-2 passages).
-
bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged at 12,000 RPM for 8 min at 37°C and cleared by passing through 0.22 μm Spin-X tube filters (Sigma–Aldrich).
-
bioRxiv - Microbiology 2022Quote: ... Adult mosquitoes of both species were provided with a sugar solution containing 8% fructose (Emprove® Chemicals, Sigma, MO, USA) ad libitum.
-
bioRxiv - Molecular Biology 2022Quote: ... cells were seeded into 12-well tissue culture trays at a density of 2×105 cells per well and pretreated with 10-8 M phorbol 12-myristate 13-acetate (PMA) (Sigma) for 72 h ...
-
bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
-
bioRxiv - Plant Biology 2022Quote: ... and incubated for 8 h followed by treatment with 5 μM EPFL6 for 1 h in the presence of 2 μM MG132 (Sigma). The ubiquitinated ERECTA was detected with an α-HA (ab18181 ...
-
bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
-
bioRxiv - Neuroscience 2023Quote: ... were first mixed on a magnetic stirrer for 30 min and then 8 drops of DMP-30 accelerator (#45348 Sigma) were added (Knott et al. ...
-
bioRxiv - Physiology 2023Quote: ... male and female mice 8-10 weeks of age were injected (i.p.) with 40 mg/kg tamoxifen (Sigma-Aldrich, T5648) for five consecutive days ...
-
bioRxiv - Physiology 2023Quote: ... The culture medium was replaced with a lentivirus viral medium supplemented with 8 μg/ml polybrene (Sigma, St. Louis, Missouri). 8 hours later ...
-
bioRxiv - Molecular Biology 2022Quote: 15-30 μg of protein extract were resolved on an 8% or 12% polyacrylamide-SDS gel and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore). Primary antibodies were used at the recommended dilutions in 5% milk or 3% BSA diluted in TBS-Tween ...
-
bioRxiv - Plant Biology 2022Quote: ... Sections of 7 to 8 mm were then transferred to slides treated with 100 mg/mL poly-L-Lys (Sigma) and dried on a warm plate at 40 °C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
-
bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in lysis buffer (50 mM Tris pH 8, 1 M NaCl, 10% v/v BugBuster (Novagen), 10 mM imidazole ...
-
bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
-
bioRxiv - Immunology 2023Quote: ... 8-10 pieces (ca. 150mg total) were placed in each cryovial containing 1mL Cryostor CS10 (Sigma Aldrich, St. Louis, MO) or 1mL 90% FBS/10% DMSO and placed in Corning CoolCell LX Cell Freezing Container (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: MIN6 cells stably expressing Cas9 were infected with this lentivirus at half volume with 8 μg/ ml polybrene (TR-1003-G, Sigma) overnight ...
-
bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
-
bioRxiv - Developmental Biology 2023Quote: ... Prior to fixation we live-stained all the embryos with either Caspase-3/7 or Caspase-8 (Supplementary Table 30) for 30 min and subsequently removed the zona pellucida using Acidic Tyrode’s Solution (Sigma-Aldrich) for 30 sec – 2 min and subsequently washed in PBS/2%BSA.
-
bioRxiv - Cell Biology 2023Quote: ... the reaction between peptide conjugate and crosslinkers (PEG-4VS and PEG-4 ACLT) was performed in stoichiometric ratio 1:1 in30mM HEPES buffer at pH 8 (Sigma) diluted in 1x HBSS (Gibco) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The mid log phase cells were diluted to 104 cells/ml (assuming 1 OD = 8×108 cells/ml) into LB with 0.005% Antifoam 204 (Sigma A6426) and 1 mg/ml BSA (Sigma-Aldrich A2153-100G) ...
-
bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... and plated on 35mm dishes (8×105 cortical cells and 4×105 hippocampal cells per dish) coated with poly-DL-ornithine (Sigma), in DMEM (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...