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Citations for Millipore Sigma :
2251 - 2300 of 2656 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the backs of 8 week old mice were shaved and 24 h later 50 µg DMBA (7,12-Dimethylbenz[a]anthracene) (Sigma, Dorset, UK) in 200 µl acetone was applied topically on the shaved area of the dorsal skin ...
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bioRxiv - Genetics 2020Quote: ... fractions of interest (8 to 14) were pooled together and incubated at 4 °C overnight with magnetic FLAG beads (Sigma, M8823) pre-conditioned with FPLC buffer ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Molecular Biology 2020Quote: ... The single cell suspension was washed once with Advanced DMEM and resuspended in transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich) and mixed with concentrated virus ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL aliquots of 50 μM K311Dha in in 20 mM NaPi (pH 8) was reacted with 1000 molar equivalents of N-acetylcysteamine (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... 15μg protein was separated by electrophoresis on a gradient (8-20%) SDS-polyacrylamide gel and transferred for 1 hour at 100V onto a polyvinylidene difluoride membrane (Millipore Sigma, catalog number IPVH00010 ...
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bioRxiv - Neuroscience 2021Quote: ... We gently lowered a glass capillary (Φ = 8 μm opening) in the targeted regions and slowly injected about 30 nl cholera toxin B subunit (CTB, 1%, Sigma-Aldrich) or virus ...
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bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Cancer Biology 2020Quote: ... RT112M or 639V cell lines were then transduced with the harvested retroviral medium diluted 1:4 with growth medium together with 8 μg/mL of polybrene (Sigma-Aldrich). Cells were then selected for 2 weeks with 200μg/mL of hygromycin.
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bioRxiv - Neuroscience 2020Quote: ... adult mice (older than 8 weeks of age) were treated intraperitoneally (i.p.) with 75 mg/kg of body weight of tamoxifen (Sigma Aldrich, T5648) dissolved in sunflower seed oil (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... To this was added 20 μL of a 10 mg/mL solution of 8-hydroxyquinoline (ACS reagent, 99%, Sigma-Aldrich: 252565) in chloroform (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal protein amounts (15-25μg) were separated on hand cast 8-20% acrylamide Tris-glycine gels then transferred to Immobilon-P® PVDF membranes (Millipore). Membranes were stained with amido black and rinsed in ddH2O for 5 minutes followed by blocking for 1h on rocker in 5% non-fat dry milk in TBST (Tris-buffered saline ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione) was purchased from Sigma-Aldrich (St. Louis, MO). 8MM-IBMX (3-Isobutyl-8-(methoxymethyl)-1-methyl-1H-purine-2,6(3H,7H)-dione ...
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bioRxiv - Physiology 2020Quote: ... a polycarbonate filter (8 μm pore size) (GE Infrastructure, Fairfield, CN, USA) was coated with a solution containing 0.2% gelatin (Sigma-Aldrich Co.) and inserted between the chambers ...
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bioRxiv - Genomics 2021Quote: The titer of lentivirus was determined by transducing 500,000 cells with varying amount (0, 0.5, 1.0, 2.0, 4.0 and 8.0 µL) of concentrated virus and polybrene (Millipore, TR-1003-G, 8 µg/mL,). Viral transduction was performed by centrifuging the lentivirus and cell combination at 1000 RCF for 90 min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: A drop of fresh culture containing control or spaghetti-like bacteria with 2 μM DiI dye (Sigma, CAS #41085-99-8) was placed on an NGM agar plate ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 8 - 20 mg/mL (Amicon Ultra 10 kDa cut off, Millipore Sigma). Protein samples were stored at 4°C until use.
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bioRxiv - Biochemistry 2021Quote: ... 400 μL lysis buffer with gentle non-ionic detergent (50 mM Tris buffer, pH 8, 150 mM NaCl, 1% IGEPAL CA-630 (Cat# I8896, Sigma Aldrich), 10% glycerol and 1x protease inhibitor (Cat# A32965 ...
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bioRxiv - Cell Biology 2020Quote: ... and osteogenic induction solution (10 mmol/L β-sodium glycerol phosphate, Sigma, USA; 10−8 mol/L dexamethasone, Sigma; 50 μmmol/L ascorbic acid, Sigma; and 0.01 μmmol/L 1,25-dihydroxyvitamin D3 ...
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bioRxiv - Developmental Biology 2021Quote: ... the fixed samples were lysed (lysis buffer: 10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% Igepal CA-630 (Sigma-Aldrich, I8896) and 1× protease inhibitor cocktail (Complete ...
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bioRxiv - Immunology 2021Quote: ... Cultured BMDMs were stimulated with heat-killed KP (MOI = 10) up to 8 h or Klebsiella LPS (1 ug/ml) (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... then 5×106 PFUs were resolved on an 8-12% SDS-PAGE gel and transferred to an Immobilon-FL polyvinylidene difluoride membrane (Millipore, IPEL00010). Membranes were probed for 30 min at room temperature or 4°C overnight with antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were passaged up to 8 generation post-transfection to eliminate the extrachromosomal donor vector and were then grown under puromycin (Sigma-Aldrich) selection (1μg/ml ...
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bioRxiv - Genomics 2019Quote: ... Wells were transduced with a lentiviral pool expressing the puromycin resistance gene from the Ef1α promoter in the presence of 8 µg/mL polybrene (Millipore Sigma) at a MOI of 1 ...
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bioRxiv - Genomics 2019Quote: Two pools of 150,000 K562 cells without Cas9 were transduced with a pool of lentivirus expressing the Puromycin resistance gene from the Ef1α promoter in the presence of 8 µg/mL polybrene (Millipore Sigma) one at an MOI of 1 and the other at an MOI of 5 ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was then resuspended in 330 μL Sorbitol buffer and 192 μL of this suspension were mixed with 8 μL of 50 mg/ml 100T Zymolase (2 mg/ml final concentration; Sigma-Aldrich) and incubated for 5 minutes at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... DCs (3 days post-bone marrow extraction) were transduced by adding lentiviral supernatants in the presence of DEAE dextran (8 µg/ml; Sigma-Aldrich) to cells for 4 h ...
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bioRxiv - Developmental Biology 2020Quote: ... the media was replaced with 8 μL of 100x concentrated library lentivirus mixed in 1 mL ESC medium with 1x polybrene (EMD Millipore). 16 h later ...
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bioRxiv - Immunology 2021Quote: ... A375 and 786O-NL cells by spin-infection (500 g for 2 hours at 37°C) with 8 μg/mL polybrene (Sigma-Aldrich). Four days post-transduction ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: We performed a reverse transduction of both HaCaT and A549 cells by adding lentiviral pHR-SFFV-dCas9-BFP-KRAB or pXPR_120 together with 8 μg/ml polybrene (Sigma-Aldrich, H9268) in DMEM ...
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bioRxiv - Genetics 2021Quote: ... cells used for immunocytochemistry were seeded at 5-8 x 104 cells per cm2 onto poly-D-lysine (PDL) (Sigma, P7405) and laminin-coated 12 mm glass coverslips in 24-well plates or PDL and laminin-coated clear bottom imaging 96-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed and injected into mice ovary orthotopically (n=8) followed by Doxycycline (2mg/ml) and 10% (w/v) sucrose (Sigma-Aldrich) in drinking water after 1 weeks of cells inoculation ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Biochemistry 2022Quote: ... mixed 1:1 with fresh medium and added to the desired cells together with 8 μg/ml Polybrene (Sigma, TR-1003).
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bioRxiv - Cancer Biology 2022Quote: ... were applied to PANC-1 cells in the presence of polybrene (8 µg/ml, Cat. no. TR-1003-G, Sigma-Aldrich) for 48 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Packaged lentiviruses were then applied to PANC-1 cells in the presence of polybrene (8 µg/ml, TR-1003-G, Sigma-Aldrich) and incubated over- night ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Bioengineering 2022Quote: ... containing guide rail shims made from cured SU-8 2025 photoresist (NC9981681, Kayaku Advanced Materials) and rendered hydrophobic by vapor deposition of ∼1 ml dichlorodimethylsilane (DCDMS, 44072, Sigma-Aldrich) in vacuo for ∼10 mins ...
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bioRxiv - Bioengineering 2022Quote: ... Finland) uMp-RW3 micromanipulator was used to control the patch pipette (resistance 4-8 MΩ) containing Ames’ solution (A1420, Sigma Aldrich) buffered with 10mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NaCl and indium titanium oxide (ITO) coated glass slides (surface resistivity 8–12 V sq-1) were purchased from Sigma-Aldrich Co ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Developmental Biology 2022Quote: ... The pellets were resuspended in aCSF and transferred to Eppendorf tubes pre-coated with 30% BSA (Sigma-Aldrich, 9048-46-8). After cell counting ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated in 1:30 rat anti-DN-cadherin (DN-EX #8, Developmental Studies Hybridoma Bank) and 1:500 rabbit anti-GABA (A2052, Sigma-Aldrich) or 1:200 rabbit anti-Lucifer yellow (A5750 ...