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Citations for Millipore Sigma :
1851 - 1900 of 2487 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the cell culture supernatant was filtered with a 0.22 µm filter and concentrated for 90 minutes by ultracentrifugation using a 50.2 Ti rotor at 150,000 x g through a 5 mL 8% OptiPrep (Sigma-Aldrich, MO) cushion to collect virus-like particles (VLPs) ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... FVB/NJ primary microglia harvested from mixed glia cultures by the shaking method were seed onto Millicell cell culture inserts with 8-μm pore filter (Millipore), and treated with 50 μM GM1 for 4h or with 10 μM L-t-PDMP or 5 μM GENZ-123346 for 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved on 8-15% SDS-PAGE depending on protein size and transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked with PBS-T (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then plated onto LabTech II 8-well glass bottom chambers modified with 0.1% Poly-L-Lysine (PLL, Sigma P8920) and allowed to adhere for at least 4 hours at 4C before performing confocal microscopy.
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bioRxiv - Biophysics 2021Quote: ... After gelation for 2 h at RT in a humidified chamber the samples were transferred into digestion buffer (8 U/ml Proteinase K (P4850, Sigma), 50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... was flowed into the chamber (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Parasites were incubtaed in 96 well plates with riboflavin analogues (compounds 1-6 were obtained from the the National Cancer Institute, DCTD/DTP/DSCB, Rockville, MD, USA, and compound 7 and 8 were purchased from Sigma). All compounds were prepared in DMSO except compound 2 which was dissolved in a mixture of DMSO and 1N KOH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Pelleted DNA was resuspended in 1 ml of TLE and washed twice by dyalisis with 8 ml of TLE using AMICON Ultra Centrifuge Filter Unit 15mL 30K (Millipore UFC903024 ...
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bioRxiv - Immunology 2022Quote: ... The T cells were resuspended at concentration of 4×106/ml in TCGF with 8 µg/ml polybrene (Sigma, 107689) and this suspension was plated at 500 µl/well in 12-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... 8-10 worms were randomly picked and aligned after placing in a drop of 10 mM tetramisole (Cat. T1512, Sigma) shortly ...
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bioRxiv - Cancer Biology 2022Quote: ... RMS cells were infected with viral particles for 24h at 37° C using 8 mg/mL of polybrene (EMD Millipore). Cells were selected for using 1 mg/ml G418 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 3X MIC and the maximum drug concentration (8 µg/mL) were stained (after reading the MIC) with 10 µg/mL of Calcofluor White (Sigma) for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... and 1-2x 104/cm2 cells were replated on a vitronectin-coated plate in Essential 8 complete medium supplemented with either 2uM thiazovivin(Sigma) or 1X RevitaCell Supplement ...
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bioRxiv - Microbiology 2019Quote: ... KSRP post-translational modifications were monitored in Huh7.5-SGR and Huh7.5 cells grown on Nunc Lab-Tek Chamber Slide™ system 8 wells (Permanox, Sigma-Aldrich), 10,000 cells/well ...
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bioRxiv - Genomics 2019Quote: ... sorted samples were re-suspended in 1ml Cell Lysis Buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40/Igepal) containing fresh protease inhibitors (Sigma P8340) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2019Quote: ... 37 °C for 90 secs and samples fixed by addition of an equal volume of 2x fixative (8% paraformaldehyde/0.015% glutaraldehyde, Sigma-Aldrich). Cells were permeabilised with 0.1% Triton X-100/PBS for 10 mins and blocked with 3% BSA/PBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Molecular Biology 2020Quote: ... and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 µg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Microbiology 2019Quote: ... The re-suspensions were spread on 7H11 OADC plates supplemented with the relevant drug concentration (2, 4, or 8 µg/mL of ofloxacin, or 100 µg/mL streptomycin; Sigma). Resistant colonies were observed and enumerated after 21 to 35 days of incubation ...
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bioRxiv - Developmental Biology 2019Quote: Embryos were recovered from dissected oviducts (up to the 8-16 cell stage) or uterine horns (blastocyst stages) by forcing Flushing and Holding Media (FHM, Millipore) through them ...
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bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
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bioRxiv - Developmental Biology 2021Quote: ... were grown on 0.1% gelatin-coated flasks in 8% CO2 at 37C and cultured in mouse ES media serum+2i+LIF: DMEM (Sigma) without phenol-red ...
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bioRxiv - Immunology 2020Quote: ... 7-to 8-week old WT mice were injected intraperitoneally with 20 mg/kg body wight of LPS (Sigma, L2630). The mice were administered intraperitoneally 200 μl of DPBS containing no antibody (n = 17) ...
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bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... 5–10 × 106 U2OS cells treated with or without 4mM HU were collected and genomic DNA was cross-linked by two rounds of incubation in 10 μg ml−1 4,5′,8-trimethylpsoralen (Sigma-Aldrich) and 3 min of irradiation with 366 nm UV light on a precooled metal block10,26 ...
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bioRxiv - Molecular Biology 2021Quote: ... The reduced and alkylated samples were buffer exchanged with 50 mM ammonium bicarbonate (pH 8) using a 50-kDa MWCO filter (Millipore) prior to trypsin digestion ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 µg/ml of streptomycin (Sigma) and 2 mM L-Glutamine (PAA Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: Cryo-sections (8-10 μm, sagittal orientation) of the AF and NP were cut and incubated with 1% hyaluronidase (Sigma) for 30 min at 37°C [20] ...
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bioRxiv - Microbiology 2021Quote: ... NoDice/ΔPKR cells were seeded at the density of 8.104 cells per well in 8-well Millicell® EZ Slides (Merck Millipore), transfected and infected as described previously ...
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bioRxiv - Developmental Biology 2019Quote: ... and were cultured for additional 8-10 days in DMEM/F12 containing 2.5% FBS and 1 μM retinoic acid (Sigma, R2625).
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bioRxiv - Microbiology 2019Quote: ... Aqueous solutions of chlorhexidine-gluconate are most stable within the pH range of 5-8 (https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c9394pis.pdf). The Mueller-Hinton broth adjusted to pH=5 did not affect the visible growth of E ...
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bioRxiv - Genomics 2021Quote: ... and the lentiviral library (MOI = 0.5) was transduced into the iPSCs with 8 µg/mL Polybrene (Millipore, TR-1003-G) and spun at 1000 RCF at 37°C for 90 min ...
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bioRxiv - Genomics 2021Quote: ... fluorescent E-barcodes were defined by patterning the SU-8 structure doped by adding 0.004 wt‰ of Rhodamine 6G powder (Sigma-Aldrich) into SU-8 precursor.
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bioRxiv - Genetics 2021Quote: ... 0.2×106 mESCs were seeded per 12-well and transduced the next day with 500 µl of viral supernatant and 8 ng/µl polybrene (Sigma). Antibiotic selection was started two days after transduction and kept for at least 3 passages.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 7-dichloro-8-hydroxyquinoline (CLCQ; D64600-Lot#STBH7389) and Zinc Chloride (ZnCl2; 208086-Lot#MKCL1763) were purchased from Sigma Aldrich. We prepared 10mM stocks solutions of the inhibitors in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... After a release for 8 h in fresh media they were incubated with 50 ng/mL nocodazole (Sigma-Aldrich, M1404) for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1 M PBS and then developed with a 8 min incubation in DAB-Nickel solution: 10 mg 3,3′-diaminobenzidine (DAB) tablet (Sigma, DF905), 0.5 ml of a 2 % aqueous nickel sulfate solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were counterstained with hematoxylin (for CD45, MPO, IBA1, CD3, Ki67, 8-OHdG, and pH2AX) or Hoechst 33258 (Sigma-Aldrich) for HMGB1 ...
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bioRxiv - Systems Biology 2021Quote: ... the membrane is blocked using 5% milk in TBS-T buffer (Tris-Buffered Saline pH 7.6 [TBS {20 mM Tris pH 8, 150 mM NaCl, Hydrochloric acid [Sigma Aldrich, 320331]}] with 0.1% Tween-20 [Fischer Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Sections (8-10 μm thick) were obtained using a Leica RM2045 microtome and stained with 0.1 % Ruthenium Red (Sigma-Aldrich) in PBS for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Interleukin 3-dependent hematopoietic pro-B cell line Ba/F3 (57) were transduced with the retroviral supernatant supplemented with 8 μg/ml of polybrene (Sigma) and IL-3 ...
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bioRxiv - Cancer Biology 2020Quote: ... 17 β-estradiol was maintained in 200-proof ethanol at 2.7 mg/ml stock solution and added to drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). Lapatinib (SelleckChem ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 or MDA-MB-231 cells were transduced with concentrated viruses in the presence of 8 ug/ml of polybrene (Sigma) overnight or spin transduced at 500g at 32 °C for 90 min followed by incubation at 37 °C for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... confluent EnSC monolayers were down-regulated in phenol-free DMEM/F□12 media containing 2% DCC□FBS and decidualized with 10 μM medroxyprogesterone acetate (MPA) and 0.5 mM 8 bromo cAMP (C+M treatment) (Sigma Aldrich). To study the effect of exogenous HA ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 μL concentrated lentivirus encoding GC-C linked to a GFP reporter was added to a total of 1 mL growth medium containing 8 μM polybrene (Sigma). 24 hours post transduction ...