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Citations for Millipore Sigma :
2201 - 2250 of 2656 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the HEK293T cell lysates transiently expressing mScarlet-I-tagged spartin truncation mutants were lysed in 500 μl of lysis buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Cell Biology 2021Quote: ... DLD1-wildtype and DLD1-NXF1-AID cells were pretreated for 0-8 hrs with 0.5 mM synthetic auxin (3-indoleacetic acid, Sigma, in ethanol), prior to addition of 250 nM NVP-2 ...
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bioRxiv - Microbiology 2020Quote: ... and fractions containing target protein were collected and concentrated to 6-8 mg/ml using Amicon concentrators (Millipore, Billerica, MA, USA), flash-frozen with liquid nitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Neuroscience 2021Quote: ... 40μg of total proteins from each sample were separated by 8∼12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA), the membranes were blocked in 5% BSA ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 1.5 ml lysis buffer (10 mM Tris-Cl pH 8, 150 mM NaCl, 1% Triton-X-100, 10 mg/ml lysozyme, 1x EDTA-free protease inhibitors [Sigma Aldrich]) and incubated for 25 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 6 mL of cold water and lysed by adding them dropwise to 6 mL of boiling 8% SDS (Sigma-Aldrich) within 10 min ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from 7-d-old Emmenanthe penduliflora seedlings grown in 16:8 photoperiod on moistened filter paper using Spectrum Plant Total RNA kit (Sigma-Aldrich) after removal of seed coats ...
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bioRxiv - Molecular Biology 2020Quote: ... female mice (8-10 weeks of age) were anesthetized by isoflurane inhalation and subsequently administered a single dose of bleomycin (Sigma-Aldrich) oropharyngeally at 0.5 mg/kg body weight in a volume of saline not exceeding 50 μl per mouse ...
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bioRxiv - Cancer Biology 2020Quote: Using the custom barcodes lentiviral shRNA library, PDX lines (PATC69, PATC124, PATC53 and PATC153) were transduced in vitro using 8 μg/mL Polybrene (Sigma-Aldrich). Libraries were transduced at 1000X coverage and multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2020Quote: ... PATC53 and PATC153) containing the lentiCas9-blast vector (addgene, plasmid #52962) were transduced in vitro using 8 μg/mL Polybrene (Sigma-Aldrich). PDX lines transduced with Cas9 were constantly kept at 10 μg/mL blasticidin (Thermo Fischer Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 8 week-old mice were injected intraperitoneally with 2 μL/gram of 25% carbon tetrachloride diluted in corn oil (Sigma Aldrich). For all time points ...
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bioRxiv - Cell Biology 2021Quote: ... wild type or Fmr1 knockout mice aged (≥8 weeks of age) were injected intraperitoneally with Corn Oil (vehicle, Sigma, USA, C8267) or with Nap dissolved in corn oil (300 mg kg−1 ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Neuroscience 2020Quote: ... the sample was rinsed in PBS with 0.05% sodium azide (PBS-azide) and decalcified by placing it in a container with 250 mL of 8% EDTA (Sigma-Aldrich) for 6-7 days at room temperature on an orbital shaker ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell culture medium was replaced with 2 mL fresh medium containing 8 µg/mL hexadimethrine bromide (Sigma Aldrich, H9268-5G), 20 µL ViralPlus Transduction Enhancer (Applied Biological Materials Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... RMS cells were infected with viral particles for 24 hr at 37°C using 8 mg/mL of polybrene (EMD Millipore).
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bioRxiv - Microbiology 2022Quote: ... One hundred thousand cells were spinoculated with 100 µl of concentrated viral supernatant in the presence of 8 µg/ml polybrene and 10 mM HEPES (Sigma-Aldrich) at 800 g ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded into 6-well plates so that they reached 50-60% confluency on the day of infection and transduced at most 3 consecutive days with the viral stock in the presence of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Immunology 2022Quote: ... or HKLM (TLR2 agonist, 108 particles/ml) for 8 hours in the presence of 5 μg/ml brefeldin A (Sigma-Aldrich). TLR agonists were purchased from InvivoGen (San Diego ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein samples were electrophoresed on 8% or 10% precast sodium dodecyl sulfate–polyacrylamide gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). The PVDF membranes were incubated with Tris-buffered saline containing Tween (TBS-T ...
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bioRxiv - Biophysics 2022Quote: ... We treated LTR-MS2 reporter cells placed in 10 cm dishes with viral supernatant supplemented with 8 μg/ml polybrene (Sigma TR1003G). 24 h later ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transduced for 1-3 consecutive days with the viral stock diluted 1:3 with culture media in the presence (or absence for BT-549 cell line) of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Immunology 2022Quote: 8-well Nunc Lab-Tek II chambered coverglass (Thermo #155360) were cleaned by submersion in 5% Hellmanex III (Sigma-Aldrich #Z805939). Flask containing chamber in solution was microwaved for 25 sec and then allowed to clean at room temperature overnight ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated in the dark for 30min with SDS buffer (100 mM Tris–HCl pH 8, 1% SDS, 250 mM NaCl, 5 mM EDTA) with 40 mM iodoacetamide (Sigma, #I1149). The resin was then resuspended in SDS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were incubated for 1 hr in picrosirius red solution [0.5 g of Direct Red 80 (Sigma-Aldrich; 2610-10-8) in 500 mL of saturated picric acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Protoplastation was performed by transferring the treated germlings to fresh phosphate-KCl buffer supplemented with 8 mg/mL of a lysing enzyme mix from Trichoderma harzianum (Sigma-Aldrich) and further incubating the mix at 30 °C (50 rpm ...
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bioRxiv - Neuroscience 2021Quote: Transgenic mice (8 days post administration of hydroxytamoxifen) were terminally anaesthetised and anaesthesia was maintained with sodium pentobarbital (60mg/kg, Sigma-Aldrich). The external jugular vein was cannulated and 1 mg per 20g tetramethylrhodamine isothiocyanate (TRITC ...
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bioRxiv - Microbiology 2020Quote: ... 48-well plate) were infected with varying dilutions of viral stocks in the presence of 8 μg/mL polybrene (Sigma Aldrich). 48 h following infection ...
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bioRxiv - Microbiology 2020Quote: Spores of fungal cultures grown at 21-23°C for 5–7 days on sporulation medium were harvested using 10 ml saline tween solution (8 g l−1 NaCl and 0.05 ml l−1 Tween 80) and filtered through Miracloth (Millipore; Billerica, USA) following the protocol described from Benoit et al ...
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bioRxiv - Neuroscience 2021Quote: Cortical synaptosomes were incubated for 90 min at 4 °C with gentle shaking on Nunc Lab-Tek 8-well chamber slides (Sigma-Aldrich) coated with poly-D-lysine (ThermoScientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... To create the U937 SLC19A1−/− cell line,16 1 mL filtered supernatant was supplemented with 8 mg/mL polybrene (Sigma Aldrich) and added to 1 × 105 cells in a 24 well plate ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... when cells were at ~70% confluency media were replaced with fresh complete media supplemented with polybrene (8 μg/ml, EMD Millipore). Ready to use lentivirus particles containing 4 different siRNAs targeting TRAF6 (Suppl ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were transduced by spinoculation at 500 g for 1 hour at room temperature with 8 μg/mL polybrene (EMD Millipore). HEK293T cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... the 22RV1 and LNCaP cells were infected with the collected lentiviral particles along with polybrene (hexadimethrine bromide; 8 µg/ml) (Sigma-Aldrich). Culture media was changed the next day and puromycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 614-96-0), 6-methylindole (97%, CAS# 3420-02-8) and 7-methylindole (97%, CAS# 933-67-5) were acquired from Sigma-Aldrich, and 4-methylindole (99% ...
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bioRxiv - Systems Biology 2022Quote: ... in HNN Buffe pH 7.8 (50 mM HEPES, 150 mM NaCl, 50 mM NaF, 200 μM Na3VO4, 1 mM PMSF, 1x Protease Inhibitors (Sigma, P8215), 1x Benzonase (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... The arenas were covered with a watch glass (7 cm diameter, 8 mm height in the center) which was coated on the inner side with Sigmacote (Sigma-Aldrich) to prevent flies from walking on the inside of the glass ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were centrifuged at 300 g for 5 minutes and resuspended in Essential 8 Flex medium containing 2 µM ROCK inhibitor Thiazovivin (Millipore Sigma). hPSCs were then counted using a Moxi Cell Counter (Orflo Technologies ...
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bioRxiv - Biophysics 2019Quote: ... Plasma membrane-specific permeabilization was achieved by 8 min incubation on ice in TRB containing 25 μg/mL digitonin (Cat#D141; Sigma Aldrich). Samples were washed twice for 5 min in TBA ...
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bioRxiv - Plant Biology 2019Quote: ... wild and transgenic seeds were grown on 0.8% agar-solidified 1/2 MS vertically oriented plates for 7 days with or without specific concentration of Propyzamide (Sigma-Aldrich). To detect the morphology of the cells of 7-day-old seedlings ...
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bioRxiv - Microbiology 2019Quote: ... The dilutions (10−3 to 10−8) were plated in triplicates on LB plates supplemented with nystatin (Sigma, 100 μg/ml) to prevent fungal growth ...
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bioRxiv - Immunology 2019Quote: C57BL/6 mice (female, 9-10 weeks old) were nebulised with LPS (3 mg per group of 8 mice) (Pseudomonas aeruginosa, Sigma-Aldrich) and immediately injected intraperitoneally (i.p. ...
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bioRxiv - Molecular Biology 2019Quote: ... exponentially growing HUVEC cells were exposed to 21% (normoxia) or 1% (hypoxia) oxygen for 8 hours and pulse labelled with 4-thiouridine (400 µM, 4sU, Sigma, T4509) during the last two hours of treatment ...
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bioRxiv - Systems Biology 2019Quote: ... were plated into a LN511-coated 12 well plate in the standard media supplemented with 8 μg/mL polybrene (Sigma-Aldrich). Each well received a virus amount equal to MOI = 0.3 ...