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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated on rat tail collagen (Sigma-Aldrich, 11179179001) coated glass slides ...
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bioRxiv - Neuroscience 2019Quote: ... HRP-conjugated secondary antibodies: Goat anti-rat was from Millipore and Jackson Immuno Research ...
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bioRxiv - Neuroscience 2021Quote: ... type I from rat tail (Cat. No. C3867, Millipore Sigma) at 10 μg/cm2 for two hours in a 37°C incubator ...
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bioRxiv - Neuroscience 2020Quote: Rats were euthanized with sodium pentobarbital (130 mg/kg; Sigma) and transcardially perfused with PBS to remove blood ...
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bioRxiv - Cancer Biology 2020Quote: ... briefly rat-tail type I collagen (#08-115, Sigma, Millipore) was prepared to a final concentration of 2.1 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... briefly rat-tail type I collagen (#08-115, Sigma, Millipore) was prepared to a final concentration of 2.1 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... Rat tail collagen I (C3867-1VL, Sigma-Aldrich, MO, USA) was diluted in DMEM/F12 media with phenol red and 1N NaOH was added dropwise until pH was around 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... A7r5 rat smooth muscle embryonic aorta cells (Sigma Aldrich, #86050803) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... fixed rat brain tissue was stained for TH (Ab1542, Millipore) followed by staining with total LRRK2 (N241A/34 ...
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bioRxiv - Molecular Biology 2020Quote: ... membrane was incubated with anti-rat IgG HRP conjugate (Sigma) diluted to 1:1000 in TBS Tween 20 buffer for 1.5 hours with constant agitation ...
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bioRxiv - Neuroscience 2021Quote: ... the rats were perfused with 4% paraformaldehyde (441244, Sigma-Aldrich), and the brains were removed from the skulls ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-Flag mAb (Sigma-Aldrich, St Louis, MI, USA), mouse anti-human CD14-APC mAb (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral constructs expressing shRNA targeting rat IP3R1 (TRCN0000321161; Sigma-Aldrich) and TRPM2 (TRCN0000068488 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... After behavioral testing concluded rats were overdosed with tribromoethanol (Sigma) and brains were immediately dissected and fresh frozen on dry ice ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were pre-treated with rat serum (Sigma-Aldrich), and incubated with anti-mouse/human CD309 (Flk-1/VEGFR2)-APC (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... the rats were anesthetized (Inactin, Sigma T133, 150mg/kg i.p.) and ...
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bioRxiv - Pathology 2023Quote: ... Contain 0.2 mg/ml rat tail collagen I (Sigma-Aldrich) solution was then layered onto the stiffness gradients polyacrylamide substrate and reacted overnight at 4℃ ...
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bioRxiv - Neuroscience 2023Quote: ... Rats were anesthetized with Inactin (Sigma T133; 150mg/kg i.p.). Once anesthetized deeply (loss of foot-pinch withdrawal reflex) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Rat heart derived H9c2 cardiac myoblasts (Sigma-Aldrich, St.Louis, MO) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to 5 mL rat serum (Sigma-Aldrich R9759) and then incubated head-over-head for 3 hr at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Collagen type I (rat tail, Sigma Aldrich, Cat# 08-115) was mixed on ice with cold MEGM to a final concentration of 1.7 mg/mL and supplemented with 0.1 N NaOH at a volume that equals 12.5% of the collagen volume ...
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bioRxiv - Molecular Biology 2023Quote: ... Horseradish peroxidase-conjugated goat anti-rat secondary antibody (Sigma-Aldrich) diluted 1:2000 was detected with SuperSignal® West Pico Chemiluminescent Substrate (Pierce ...
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bioRxiv - Genetics 2023Quote: ... cells were incubated with FITC anti-rat IgG (Sigma-Aldrich) and AF633 anti-rabbit IgG (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438) secondary antibodies (1:200 dilution in TTBS-milk ...
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bioRxiv - Neuroscience 2024Quote: ... rat monoclonal antibody against Dopamine Transporter (Millipore Cat# MAB369, RRID:AB_2190413). Goat anti-Mouse IgG Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-11001 ...
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bioRxiv - Cell Biology 2022Quote: ... incubated at room temperature with secondary antibodies coupled to horseradish peroxidase (1:20,000 Thermo Fisher goat-anti-rabbit, or 1:20,000 Millipore Sigma goat-anti-rat in 5% nonfat dry milk). Membranes were washed again in PBS containing 0.1% Tween 4 x 15 min ...
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bioRxiv - Biophysics 2021Quote: ... Next, dissolved membrane proteins (1 nmol) and MSP (50 μL, 1 mg/mL) (MSP1E3D1, Sigma-Aldrich, No. M7074) were added to the lipid suspension and mixed for ∼1 h while rotating in the dark at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, Cat. No. I6758) and occurred overnight at room temperature under shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins (0.5-1 mg) were precipitated using the anti-FLAG antibody (1:100, Cat. #F7425; Sigma-Aldrich), anti-HA antibody (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were then diluted with TE (1:1) and RNA and proteins were digested (RNAse, Sigma-Aldrich® ...
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bioRxiv - Genomics 2020Quote: ... 20 µl protein extracts diluted 1:1 (v/v) with ice-cold 2x Laemmli sample buffer (Sigma-Aldrich) were separated by 12% SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein samples were diluted at a ratio of 1:1 in Laemmli sample buffer (Sigma) and separated on pre-cast gel (4–12% Tris–glycineBio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton 100 and 1 × protein inhibitor) and subsequently precipitated with anti-Flag M2 Magnetic beads (Sigma, M8823) or GFP-Trap (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... Eluted proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting: hDAT and DAT/C-S were detected with amino-directed rat anti-DAT (MAB369, Millipore, 1:2000), and N-S/DAT and S/DAT/S were detected with amino-directed mouse anti-hSERT (MAb Technologies,1:2000) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein levels were normalized to β-actin (1:5000 dilution, A5060, Sigma Aldrich). Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by western blot using anti-FLAG (1:1,000 dilution; Sigma), anti-hemagglutinin (HA ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...