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Citations for Millipore Sigma :
1601 - 1650 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by detection with HRP-conjugated secondary antibodies and chemiluminescent substrate (Immobilon Western; Millipore-Sigma, Burlington, MA). Bands were visualized with Azure 600 Imaging System (Azure Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were developed by enhanced chemiluminescence detection system using Immobilon® Forte Western HRP Substrate (Millipore, #WBLUF0500) on Bio-Rad ChemiDoc™ Touch Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Detection of ɣ-Tubulin or pS87 MUS81 was performed using anti-ɣ-Tubulin (1:200, Sigma-Aldrich) or anti-pS87MUS81 (WB 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Neuroscience 2024Quote: ... The genomic region containing CRISPR-edits was PCR amplified from genomic DNA template using RedExtract PCR mix (Sigma). Amplicons were purified from PCR buffer using Monarch PCR cleanup kit (New England Biolabs ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... and permeabilized for 10 min at RT in PBS supplemented with 0.1% Triton X-100 (Sigma Aldrich). Cells were blocked in filtered PBS supplemented with 0.1% Triton X-100 and 5% BSA for 1 h ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... and permeabilized for 10 min at RT in PBS supplemented with 0.1% Triton X-100 (Sigma Aldrich). For blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... reduced with 10mM DTT for 1h at RT followed by alkylation with 40 mM Iodoacetamide (Sigma, U.S.A.) for 1h at RT under dark conditions ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The dechorionated embryos were fixed for 15-20 min at room temperature (RT) with 2% formaldehyde (Sigma) in 5 ml fixing solution (50 mM HEPES-pH-7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... endogenous peroxidase was blocked by incubating for 10 min at RT in 0.3% H2O2 (Sigma-Aldrich #216763). Sections were then incubated in biotinylated goat anti-rabbit antibody (Abcam #ab6720 ...
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bioRxiv - Neuroscience 2020Quote: ... the epidermal sheet was separated from the dermis and incubated at RT in 50% EDTA (Sigma-Aldrich) and 0.05% trypsin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 200-250ng of chromatin were digested for 7 min at RT with decreasing amounts of MNase (Sigma). A 0.5U/µl stock was diluted 1:18 ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were performed in triplicate using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma). Primers used were ...
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bioRxiv - Biochemistry 2020Quote: ... Mixture incubated at RT for 5 minutes and desalted using 30 kDa MWCO spin filters (Millipore-Sigma) into 150 mM ammonium acetate for MS analysis.
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bioRxiv - Biochemistry 2020Quote: ... Mixture incubated at RT for 5 minutes and desalted using 30 kDa MWCO spin filters (Millipore-Sigma) into 150 mM ammonium acetate for MS analysis.
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bioRxiv - Pathology 2021Quote: ... Cryosections were blocked for 90 min at RT with TBS containing 0.2% Triton X-100 (Sigma-Aldrich) and 10% bovine serum (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were blocked for 2h at RT (3% normal donkey serum [Sigma D9663], 0.1% triton-x), stained with 1’ ab for 12h at 4°C (EMD Millipore [ab144P ...
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bioRxiv - Biochemistry 2022Quote: ... RT overnight) before derivatising with 20 µl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane (Sigma, 33148) for ≥1 h ...
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bioRxiv - Neuroscience 2021Quote: ... were fixed for 15 min at room temperature (RT) in 4% (w/v) paraformaldehyde (PFA, Sigma-Aldrich) in distilled phosphate buffered saline (dPBS ...
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bioRxiv - Biochemistry 2021Quote: ... LtaA mutants were incubated for 3h at RT in the presence of 0.5 mM mPEG5K-Maleimide (Sigma) and 0.5% SDS ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from frozen gastrocnemius muscle powder using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... baltica strain #1432 was grown at room temperature (RT) on agar plates (12 g sea salt (Sigma), 1 g yeast extract (Helicon) ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked for 1 hour at room temperature (RT) in PBS containing 2% bovine serum albumin (Sigma-Aldrich). Fixed cells were then incubated with the primary antibody in 2% BSA/PBS solution overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were blocked for 2 hours at RT with blocking solution (PBS with 1% chicken ovalbumin; Sigma). For ELISA inhibition assay ...
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bioRxiv - Immunology 2020Quote: ... for 1 hr at RT and then enzymatically visualized by 3,3’-diaminobenzidine (DAB) enzyme reaction (Sigma-Aldrich). Sections were counterstained with hematoxylin and eosin and images were captured using Zeiss ZEN software on a Zeiss Cell Observer inverted microscope with an Axiocam dual B/W and color camera with a 20x objective ...
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bioRxiv - Neuroscience 2021Quote: ... Japan) were permeabilized at RT for 40 min in PBS containing 0.5% Triton X-100 (Sigma-Aldrich). Sections were then blocked overnight at 4°C in PBS containing 10% normal horse serum (NHS) ...
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bioRxiv - Biochemistry 2020Quote: ... incubated for 2 hours at RT with primary monoclonal mouse anti-FLAG M2 antibody (F1804, Sigma Aldrich) to track migration of 3 x FLAG-tagged eIF2B complex ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 100 mg of tissue or 106 cells were homogenized in 1 mL RNAzol RT (Sigma #R4533) and mRNA was extracted according to the product technical bulletin ...
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bioRxiv - Biochemistry 2022Quote: ... RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... and blocked (2% bovine serum albumin, Sigma-Aldrich, 0.25% Triton-X, in PBS; 30 min. at RT). Samples were incubated overnight at 4 °C with primary antibodies against lamin-B1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... at room temperature (RT) for 20min and then blocked in 2% Bovine Serum Albumin (BSA, Sigma, SLBT8252) in 1X PBST for at least 60 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 10 min at RT with 0.1% [w/v] sodium borohydride (NaBH4, Sigma 452882) in PBS to reduce GA ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were blocked at RT for 1 hour with 5 % milk diluted in PBS (Sigma-Aldrich, P4417) containing 0.1% Tween-20 (PBS-T) ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Pathology 2023Quote: ... with colon fragments being collected and fixed 8 h (RT) in 4% paraformaldehyde (P6148, Sigma Aldrich, USA). Tissue dehydration ...
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bioRxiv - Cancer Biology 2023Quote: ... background signal reduction through incubation for 10 minutes at RT in 0.1 % Sudan Black (Sigma-Aldrich, 199664) took place and sections were washed thoroughly in TBS-T ...
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bioRxiv - Biophysics 2023Quote: ... in PBS for 10 minutes at RT and (2) treated with 0.1 M Hydrochloric acid (Sigma Adrich) for 5 minutes at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... with each wash for one hour at RT and then incubated overnight with Hoechst 33342 (Sigma, B2261) and species-specific secondary antibodies Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then refixed in 4% PFA for 5 min at RT then incubated in 2xSSC (Sigma) for 2 x 5 min RT ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were cloned into pET28a (Novagen), pET28 His6 Sumo TEV LIC (1S ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... PCR was performed using invadolysin primers (Sigma) designed to selectively amplify the invadolysin transgene ...
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bioRxiv - Developmental Biology 2019Quote: ... and PCR Grade Proteinase K (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... 1x PCR buffer (pH=8.7; Sigma, Germany) and 1 μl (30–50 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 8.75 µl of PCR water (Sigma). Reactions were performed on an Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... PCR II products were purified by Millipore MultiScreen® PCRμ96 plate according to manufacture instructions and eluted in 30 μL of nuclease-free water (DEPC) ...
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bioRxiv - Cell Biology 2021Quote: ... PCR primers were purchased from Sigma-Aldrich. Zebrafish primer sequences have been previously published (Lim et al. ...
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bioRxiv - Neuroscience 2019Quote: RedTaq ReadyMix PCR Reaction Mix (Sigma Aldrich), 1 µl of cDNA (obtained as described above ...
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bioRxiv - Immunology 2022Quote: ... The PCR products and pET16b vector (Novagen) were digested with NdeI and XhoI at 37°C for 2 hours ...