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Citations for Millipore Sigma :
1401 - 1450 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and incubated for 1h at RT with 1% BSA + 0.3% TritonX-100 (Sigma Aldrich T9284) in DPBS ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated in primary antibody for 1 hour at RT: rabbit Anti-GLUT1 (Millipore), chicken Anti-GFP (Aves) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were further washed at RT for 5min with 1mL 100µM Ammonium bicarbonate (Sigma-Aldrich), 10mM DTT and then with 1mL 100µM Ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated overnight at room temperature (RT) with anti-VRK3 (1/1000 #HPA056489, Sigma) and anti-Phalloidine (1/200 #8940S ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections were stabilized at RT for 5 min and blocked with 10% donkey serum (Sigma) + 0.3% Triton X-100 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by 10 min fixation at RT by adding 20 μL of 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated for 2 h at RT in casein blocking buffer (Sigma Aldrich), then overnight at +4°C with rabbit anti-CtBP2 antibody (Abcam #ab128871 ...
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bioRxiv - Cell Biology 2023Quote: Macrophages were fixed with 4% formaldehyde for 20 min at RT in the dark (Sigma). After fixation ...
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bioRxiv - Biochemistry 2024Quote: ... for 10 min at RT and permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, T8787) in TBS [0.15 M NaCl (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were fixed with 4% paraformaldehyde in PBS pH 7.4 (Sigma Aldrich, 20 min, RT), permeabilized with acetone (3 min ...
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bioRxiv - Cell Biology 2024Quote: ... the gels were stained 30-60 min at RT with EzBlue staining reagent (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and RT-qPCR was performed as described previously [48] using QuantiTect SYBR Green PCR kit and KiCqStart SYBR Green optimized primers from Sigma-Aldrich (KSPQ12012).
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bioRxiv - Developmental Biology 2020Quote: DIG-labeled riboprobes were generated using 2ul of PCR template with the Roche DIG RNA Labeling Kit (T7) (Sigma aldrich, SKU 11277073910)
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... DNA amplicons and related donor template levels were evaluated by semiquantitative PCR and qPCR (using KAPA SYBR FAST qPCR Kits, Sigma-Aldrich, USA), respectively ...
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bioRxiv - Genetics 2023Quote: ... blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (Supplementary Table 2) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich; 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were filled to a total 25 µL with PCR grade water (Sigma-aldrich) then placed on a ThermalCycler ...
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bioRxiv - Physiology 2020Quote: PCR reactions were performed using REDTaq® ReadyMix™ PCR Reaction Mix (Sigma-Aldrich, cat. R2523) with the primers listed in Table 1 (500 nM of each ...
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bioRxiv - Cancer Biology 2021Quote: ... Nested PCR was performed by two successive PCR reactions using KOD Xtreme polymerase (EMD Millipore #71975) and following set of primers to amplify the genomic region of INSM1-3’UTR:
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bioRxiv - Cell Biology 2020Quote: PCR-based homologous integration cassettes were amplified using the ExpandTM High Fidelity PCR system (Millipore Sigma) from the appropriate plasmid with 80 bp flanking homology to replace the 3’ end of mto2 (primers designed with PPPP at www.bahlerlab.info/resources/)(Bähler et al. ...
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bioRxiv - Microbiology 2023Quote: The DNA was then subjected to conventional PCR using JumpStart REDTaq ReadyMix PCR Reaction Mix (SIGMA) and genus-specific primers for bacteria detection in human lung tissues ...
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bioRxiv - Immunology 2023Quote: The purified libraries were indexed by PCR using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004) and sample-specific i5 primers (10x genomics PN2000095 ...
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bioRxiv - Neuroscience 2024Quote: ... Knock-in alleles were then PCR amplified from genomic DNA templates using RedExtract PCR mix (Sigma), and amplicons were visualized on agarose gels with SybrSafe (Thermo Fisher).
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bioRxiv - Cell Biology 2022Quote: ... rinsed three times with PBS (with MgCl2 and CaCl2, Sigma D8662), then fixed for 10 min in 3% Methanol-free PFA (Alfa Aesar ...
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bioRxiv - Developmental Biology 2019Quote: ... washed 3 times and incubated with streptavidin Cy3 (Sigma, 1:500) for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... and immunoprecipitated a second time using FLAG M2 antibody (Sigma-Aldrich) and Protein G magnetic beads (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed 3 times with PBS (Merck Millipore, Billerica, Massachusetts, USA) containing 0.32 % (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... retention times and co-injection spiking of authentic standards (Sigma, Carotenature). Quantification was performed based on β carotene calibration curves prepared with an authentic standard (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... and gently washed three times with 0.1% Saponin (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Biophysics 2020Quote: ... Cells were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before blocking with 5 % BSA (Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times with PBS-Tween 20 (1379, Sigma) 0.1% and incubated with phalloidin for 30 min and DAPI (D9542 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Pellets were rinsed three times in Milli-Q (mQ) water (Millipore). After the final centrifugation ...
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bioRxiv - Pathology 2022Quote: ... Samples were washed three times and incubated with DAPI (Sigma, B2883) for 1 minute ...
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bioRxiv - Genetics 2019Quote: ... washed in PBS 3 times and mounted with Fluoroshield™ (Sigma). Images were acquired on an Axio Observer microscope (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... After washing three times with PBS containing 0.1% Tween-20 (Sigma) the membranes were developed using ECL reagent (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were treated with 1µM flavopiridol for the indicated times (Sigma). siRNA transfection was performed using the Dharmafect 4 reagent (Dharmacon ...
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bioRxiv - Microbiology 2021Quote: ... The filters were rinsed 3 times in TBS + 0.05 % Tween (Sigma), followed by one final rinse in PBS before being dried overnight at room temperature and scanned on a LI-COR Odyssey Infrared Imager model 9120 ...
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bioRxiv - Genomics 2020Quote: ... Samples were washed 3 times in 30KDa amicon columns (MilliPore # UFC5030BK) and amplified with 10 PCR cycles.
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bioRxiv - Biophysics 2020Quote: ... Cells were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before blocking with 5 % BSA (Roth ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with DMEM (D4947, Sigma-Aldrich), supplemented with GlutaMAX (35050061 ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-FLAG antibodies (Sigma-Aldrich, St. Louis, USA; 5,000 times dilution), anti-MYC antibodies (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2022Quote: ... anti-HA antibodies (Sigma-Aldrich, St. Louis, USA; 2,000 times dilution), or anti-GFP antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times in 1x PBS (phosphate buffered saline; EMD Millipore) and placed in 30% sucrose for ∼1h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Spheres were dissociated at each time point using Accumax (Sigma Aldrich) and live cells were sorted using the BD FACS Aria II.
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bioRxiv - Molecular Biology 2022Quote: ... and three times with milliQ before mounting in Mowiol (Sigma, #81381). After overnight hardening of the mounting medium at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 3 times with PBST and SigmaFAST OPD (Sigma) added ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed three times with 1M Sodium Acetate solution (Sigma 711096) and three times with phosphate buffered saline (PBS) ...