Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: 12 to 14-week-old mice were treated with antibiotic mixture mixture (chloramphenicol (Sigma, C0378-25G), spectinomycin dihydrochloride pentahydrate (Sigma ...
-
bioRxiv - Bioengineering 2022Quote: ... The remaining non-reacted cysteine residues were blocked by alkylation using 14 mM iodoacetamide (IAA, Sigma) in PBS at pH 8 (on a rotating platform (100 rpm) ...
-
bioRxiv - Plant Biology 2024Quote: ... 70% humidity) for 14 days and watered with either ultra-pure water (Merck Millipore, Burlington, USA), Peter’s fertilizer (ICL specialty fertilizers ...
-
bioRxiv - Physiology 2024Quote: ... tissues were washed for 3x30 minutes in water before decalcification in 14% EDTA (Sigma- Aldrich, E5134), pH 7.4 for 2-weeks ...
-
bioRxiv - Cancer Biology 2024Quote: ... 14 mio Lenti-X 293T cells (TakaraBio) were seeded in 18 ml complete DMEM medium (Sigma) into 15-cm culture dishes ...
-
bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
-
bioRxiv - Immunology 2021Quote: ... The membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-PALS-5 diluted 1:1,000 and mouse anti-tubulin (Sigma, catalog number T9026) diluted 1:3000 in blocking buffer) ...
-
bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
-
bioRxiv - Bioengineering 2021Quote: ... The gel compartment was removed after 1 to 5 days of incubation (depending on fusion) by adding 4 w/v% sodium citrate (Sigma Aldrich Inc.) for 15 min as a lyase for alginate (Supplementary figure 5 ...
-
bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
-
bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
-
bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
-
bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
-
bioRxiv - Neuroscience 2022Quote: ... The aqueous phase was transferred to a tube containing 500 μl of isopropanol (Fisher, A416P-4) and 5 μg of glycogen (Sigma, G1767-1VL). Then samples were pelleted at 18,213 x g for 15 min ...
-
bioRxiv - Immunology 2022Quote: ... and was further purified by centrifugation at 10,000 xg for 5 minutes at 4°C through an Amicon Ultra 0.5 ml 100 kDa cutoff spin filter (Millipore, Billerica, MA, USA). The NEU3 was analyzed for size and purity by PAGE on ...
-
bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
-
bioRxiv - Bioengineering 2021Quote: ... tissue was washed 2X in methanol 100% for 4hrs and bleached overnight at 4°C in 5% hydrogen peroxide (Sigma-Aldrich 216763) in methanol ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml; Sigma-Aldrich, www.sigmaaldrich.com). We measured mass spectra using an Ultraflex III MALDI-TOF (Bruker Daltonics ...
-
bioRxiv - Biophysics 2020Quote: ... 20 µL of 10 % Tween® 20 and 20 µL of a potassium phosphate buffer (4:5 mixture of 1 M monobasic and dibasic potassium phosphate, Sigma Aldrich, USA) were added as well as 10 µL of a 2 nmol thiol-modified single-stranded DNA solution (5’-T20-SH-3’ ...
-
bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
-
bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
-
bioRxiv - Bioengineering 2022Quote: ... The reaction was started by adding 30 µL of 100 mM (final concentration 5 mM) substrate 4-nitrophenyl β-D-glucopyranoside (Sigma-Aldrich). At regular five-minute intervals ...
-
bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
-
bioRxiv - Plant Biology 2022Quote: ... Individual plants of similar size were transferred onto BCDATG agar (4-5 per plate) supplemented with NaCl or methyl viologen (Sigma-Aldrich, 856177) and incubated at 22±1°C with a 16h:8h light:dark cycle ...
-
bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
-
bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
-
bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
-
bioRxiv - Cancer Biology 2024Quote: Mice were treated topically on postnatal day 2.5 and 3.5 with 100 μl of an acetone solution containing 5 mg/ml 4-hydroxytamoxifen (4OHT) (Sigma, St Louis, MO, USA). Neonatal mice were irradiated on postnatal day 3.5 as previously described (Recio et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... The cuticle’s pigmentation was bleached with 20% peroxide for 4-5 hours and the exoskeleton and tendons were stained with Congo Red (0.5 mg/mL, Sigma-Aldrich #C676-25G) overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... Post-fixation and sequential decalcification were carried out at 4°C by immersing in the same fixative and with the addition of 5% EDTA (Sigma-Aldrich, US) for 5 hours each ...
-
bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 5 µl of the sample solution was mixed with 5 µl of a saturated solution of α-cyano-4-hydroxycinnamic acid matrix for MALDI-MS (Sigma-Aldrich) in acetonitrile ...
-
bioRxiv - Physiology 2023Quote: ... Fixed cells were blocked and permeabilized overnight at 4°C in block solution (1x PBS + 5% bovine serum albumin (Sigma-Aldrich #A3803) + 0.5% Triton-X-100) ...
-
bioRxiv - Cancer Biology 2023Quote: ... were recovered from Matrigel using 3 mM EDTA in DPBS, washed, centrifuged (200g, 5 min, 4°C) and lysed with 1x RIPA buffer supplemented with PPI (1:100, Sigma- Aldrich, 04693132001), and benzonase (1:100 ...
-
bioRxiv - Molecular Biology 2023Quote: ... to an optical density at 600 nm (OD600) of 0.5 before 5-mL aliquots were treated with 180 uM 4-DPS (Sigma; Cat# 143057-1G) for 20 min at 30°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
-
bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
-
bioRxiv - Neuroscience 2024Quote: ... 4-5 non-embedded organoids at day 30 of organoid culture were pooled per sample and dissociated using Accuatse (Sigma, cat.no. A6964). Samples were first incubated with Accutase for one hour at 370C under dynamic conditions ...
-
bioRxiv - Biochemistry 2024Quote: ... blotted and incubated overnight in 5% skim milk (GERBU Biotechnik, 70166) in TBST at 4°C with an anti-RAS antibody (Millipore, 05-516). As a loading control ...
-
bioRxiv - Neuroscience 2024Quote: ... Retinas were quenched in 100 mM glycine in 1X PBS for 30 min at 4°C and then incubated in SUPER block buffer (15% NGS, 5% bovine serum albumin (BSA) (Sigma, Cat# B6917) + 0.5% BSA-c (Aurion ...
-
bioRxiv - Cell Biology 2024Quote: ... were assayed in a final volume of 50 µl with 5 mM 4-methylumbelliferyl(MU)-N-acetyl-β-d-glucosaminide (Sigma-Aldrich), 5 mM 4-MU-α-d-galactopyranoside and 5 mM 4-MU-β-d-galactopyranoside (Calbiochem ...
-
bioRxiv - Cell Biology 2020Quote: ... Chromatin was diluted 10 times and incubated overnight at 4 °C with 20 µL Protein A/G MagnaChIP beads (Millipore) and 8 µg anti-H3K27me3 (Millipore ...
-
bioRxiv - Immunology 2021Quote: ... Cells supernatants were centrifuged at 12856 xg for 30 seconds at 4°C and concentrated by centrifugation at 11000 xg for 30 min at 4°C through a column with a 10 kDa cut-off (Merk-Millipore). Cell lysates and concentrated supernatants were mixed with loading buffer (Sigma) ...
-
bioRxiv - Developmental Biology 2021Quote: Cryosections (10 μm) were adhered to poly-L-lysine-coated slides and fixed in 4 % paraformaldehyde (Cat. # P6148, Sigma-Aldrich) solution in PBS for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... NAD-capture was carried out with 50 μg total RNA treated with 10 μL 4-pentyn-1-ol (Sigma-Aldrich) and 3 U Adenosine diphosphate-ribosylcyclase (ADPRC ...
-
bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
-
bioRxiv - Molecular Biology 2021Quote: ... were plated onto 10 cm cell culture dishes at densities of 4×106 cells and incubated with or without PR inhibitor (10 μM Lopinavir, Sigma). HIV production was activated by adding Vorinostat (10 μM ...