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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Glass bottom dishes (14 mm φ, Matsunami) were coated with poly-D-lysine (Sigma-Aldrich) and washed with sterile dH2O three times ...
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bioRxiv - Cancer Biology 2024Quote: ... 14-pentadecenoic acid (C15:1n-1, Cayman Chemical, Cat# 19724 or Sigma-Aldrich, Cat# AMBH93E4C3FF), 14(Z)-eicosenoic acid (cis-14-C20:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the media was quickly aspirated and cells were washed three times with quenching buffer (DPBS supplemented with 10 mM sodium ascorbate, 5 mM Trolox [Sigma-Aldrich], and 10 mM sodium azide). Cells were then scraped in quenching buffer ...
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bioRxiv - Microbiology 2024Quote: ... strains were streaked from cryostocks on LB agar (10 g L-1 tryptone, CRITERION™, 5 g L-1 yeast extract, Sigma-Aldrich and 5 g L-1 NaCl) and incubated for 2 days at 30 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with 10% FCS in PBS and resuspend in Dapi solution (Sigma; 50μg/ml in 10% FCS in PBS; 100μl/well) and stored at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was centrifuged at 5,000 g for 5 minutes at 4 °C and the supernatant was added to ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich), equilibrated with the wash buffer (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and 10x DMEM (First Link Ltd., UK) were mixed at a 4:1 volume ratio and neutralized with 5 M NaOH (Sigma, Canada). Fast Green (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Neuroscience 2021Quote: ... The flies were then transferred into vials containing Drosophila instant food (Formula 4-24 Instant Drosophila Medium, Carolina Biological Supply Company, USA) containing 5% H2O2 (Sigma, H1009), or vials containing Drosophila instant food only as a control ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS (1x PBS, 5 min) and then fixed in 4% PFA (Paraformaldehyde, Sigma Aldrich, Dorset, UK) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... female mice between 4 and 16 weeks old were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Sigma, G4877) and 48 hours later ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then counterstained (5 min) with 0.5 µg/ml 4’,6’-diamino-2-phenylindole (DAPI; Sigma-Aldrich® Cat#D9542) in PBS ...
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bioRxiv - Cancer Biology 2020Quote: PKO cells transfected with Flag-tagged OGT before cultured in 5 mM glucose were collected and incubated overnight at 4°C with ANTI-FLAG M2 affinity gel (Sigma, A2220). Immunoprecipitated complexes were eluted and stained with Coomassie blue ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cultured for one week in 4 ml YNB media with a final concentration of 5 mM Nicotinamide (Sigma I17451). For copper treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... alongside a topical treatment for the tail skin (tails were dipped for 5 seconds in 25 mg/ml 4-hydroxytamoxifen (Sigma H6278) in DMSO) ...
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bioRxiv - Pathology 2023Quote: ... Freshly resected left ventricular tissue (∼2.5-4 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 µg/mL, Sigma, 47036) 30 min at 40C in BIOPS buffer containing the following ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Neuroscience 2024Quote: Mice aged 3 to 5 months were deeply anesthetized and transcardially perfused with 50mL PBS followed by 50mL 4% (w/v) paraformaldehyde (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
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bioRxiv - Microbiology 2023Quote: HLCs were infected at day 18 of the differentiation protocol at an MOI of 5 infectious units (IU) in the presence of 4% polyethylene glycol 8000 (PEG, Sigma-Aldrich) and 1.5% DMSO (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were exposed for six hours to virus with an MOI (multiplicity of infection) of 5 viral particles/cell in the presence of 4 μg/mL polybrene (Sigma-Aldrich). Cells were then washed ...
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bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Cell Biology 2022Quote: ... followed immediately by an equal volume of a freshly-prepared 5 mg/mL solution of 4-hydroxy-α-cyano-cinnamic acid (Sigma) in 50% aqueous (v:v ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... counter-stained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 minutes at room temperature and mounted with Fluoroshield (Sigma-Aldrich).