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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The cells were then re-suspended in 4 ml of supplemented PBS containing 10 μg.ml-1 of propidium iodide (Sigma Aldrich) and 100 μg.ml-1 of RNaseA (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C with a SW28 rotor in a Beckman XL-70 centrifuge or concentrated using an Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore). Pellets were resuspended in 500 µL PBS ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in PBS with 4% paraformaldehyde for 20 min at room temperature and blocked in 10% normal goat serum (Sigma). Collagen was detected using a mix of antibodies against collagen types I ...
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bioRxiv - Microbiology 2021Quote: ... 4 mg of Arixtra was added to 10 volumes (w/w) of N-Methy-N-(trimethylsilyl)-trifluoroacetamide (MTSTFA, Sigma, ≥98.5%) and 100 volumes (v/w ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mm petri dish and fixed for 10 min in 4% formaldehyde (Mecalab) diluted in PBS (in mM: 137 NaCl, 10 Na2HPO4, 2.7 KCl, 2 KH2PO4; all Sigma-Aldrich) at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cell Biology 2021Quote: ... After sonication (Bioruptor, 10 min, cycle 30/30 sec) and centrifugation (4°C, 15 min) anti-FLAG agarose beads (M2, Sigma) were added to the supernatant and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... the Phos-tag gels were incubated 4 × 10 min in 100 mM EDTA before electrophoretic transfer onto Nitrocellulose membrane (Sigma). Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Leucocytes at 1×106 were then kept in short period culture (4 h) with Brefeldin A (10 μg) (Sigma-Aldrich), 5 μg PMA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 66 These transgenic mice express the GEVI in cortical layer 2/3 pyramidal neurons under the intersectional control of TetO and destabilised Cre-recombinase.70–72 The destabilised Cre-recombinase was stochastically re-stabilised to induce sparse expression of the voltage indicator via two IP injections of a total of 2 × 10−4 mg kg−1 Trimethoprim (TMP, Sigma) over 2 consecutive days as described in Song et al ...
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bioRxiv - Cancer Biology 2021Quote: ... and EV-containing fractions were pooled and concentrated with a 10 kDa molecular weight cut-off Amicon Ultra-4 centrifugal filter unit (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: EM grids were pre-coated with 4 µl of 0.1 mg/ml BSA with 10-nm colloidal gold beads (Sigma G1527) in ddH2O and air dried for either 3 hours or overnight before use ...
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bioRxiv - Developmental Biology 2022Quote: 10 AB strain wild type embryos per condition per experiment were fixed in either 4% paraformaldehyde (PFA, Sigma Aldrich F8775) in 1X phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
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bioRxiv - Cell Biology 2022Quote: ... mice were fasted for 4 hours followed by gavaging of 2 g/kg (10 ml/kg) of D-glucose (Sigma) dissolved in distilled water ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... solids were separated by centrifugation (10 min at 20,000g at room temperature) in a Sigma 4-16K Centrifuge (Sigma Laborzentrifugen), and re-extracted with an additional 0.5 ml extraction mixture ...
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bioRxiv - Physiology 2020Quote: ... ERα was immunoprecipitated overnight at 4°C using 10 µg of Flag antibody (clone M2, Sigma-Aldrich, St. Louis, MO). Complexes bound to the antibody were captured with protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.4 mM dNTPs mix, 10 x primer master mix (1.6 µM FIP / BIP, 0.2 µM F3 / B3) (Sigma, HPLC purified), 1 x EvaGreen dye (Biotium) ...
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bioRxiv - Systems Biology 2022Quote: The cells were fixed with 4% paraformaldehyde at 37 °C for 10 - 15 min and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... Then the cells were fixed with 4% paraformaldehyde at 37 °C for 10 - 15 minutes and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then fixed with 4% paraformaldehyde at 37 °C for 10 - 15 min and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 min ...
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bioRxiv - Immunology 2022Quote: ... the cell lysates were centrifuged at 13,000g for 10 min at 4 °C and immunoprecipitated with anti-FLAG M2 affinity gel (Sigma-Aldrich). Immunoprecipitates were resolved by SDS-PAGE ...
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bioRxiv - Bioengineering 2020Quote: Mature brain organoids cultured for 30 days were allowed to attach to fibronectin glass coverslips for 4 hours in the presence of 10 μg/mL of laminin (Sigma). Attached brain organoids were then incubated on coverslips for 3 days under DMSO vehicle or THC (1 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in 24-well plates (4⨯104/well) then incubated for 72 hours in the presence of tamoxifen (0-10 μM, Sigma). Cell growth in the absence or presence of tamoxifen was monitored (every 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Transduction was performed by incubating cells with medium containing the lentivirus at a MOI of 10–100 in the presence of 4 μg/ml Polybrene (Sigma) for 12–24 h ...
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bioRxiv - Microbiology 2021Quote: ... Samples with the highest purity were pooled together and concentrated by centrifugation at 4°C with Amicon Ultra Centrifugal Filter Devices of 10 000 MWCO (Millipore). All protein versions were purified at least twice to ensure reproducibility of the results ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were labeled in media for 10 minutes with 500 µM 4-thiouridine (4sU, Sigma-Aldrich, St. Louis, MO, USA.). RNA extraction was performed with TRIzol (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: For whole mount immunolabeling adipose tissue biopsy was fixed in 20ml phosphate-buffered saline (PBS) with 4% formaldehyde (Fisher Chemical) and 10% sucrose (Sigma) in a 50ml falcon tube overnight at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2020Quote: ... cryo-sections and cells were fixed in 4% PFA for 10 min and permeabilized with 100% cold acetone (#32201, Sigma) for 6 min at −20°C or 100% cold Methanol (#32213 ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight and then blocked with 10% fetal calf serum at 4°C overnight followed by six washes with PBS containing 0.2% Tween 20 (Sigma-Aldrich). Prepared lectin plates were then incubated at 4°C overnight with a transmembrane deleted form of the MARV GP Popp strain (Lake Victoria MARV ...
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bioRxiv - Bioengineering 2020Quote: ... One solution contained a 10% w/v 4-arm PEG-vinyl sulfone (20 kDa, JenKem USA) in 300 mM triethanolamine (Sigma), pH 8.25 ...
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bioRxiv - Plant Biology 2021Quote: ... Then the proteins were concentrated to 10 mg/ml using the 30 kDa cut-off Amicon Ultra-4 centrifugal filter unit (Millipore), and stored at −80 °C for crystallization.
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bioRxiv - Biophysics 2020Quote: ... The 3F6H-fused ZTL variants were precipitated (at 4 °C for 10 minutes under dim light) with anti-FLAG antibody (Sigma), which was bound to Protein G-coupled magnetic beads (Dynabeads Protein G ...
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bioRxiv - Bioengineering 2020Quote: ... or overnight with 10 μg/ml PDL followed by at least 1 hour incubation with 4 μg/ml laminin (Sigma) for cortical neurons.
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bioRxiv - Immunology 2021Quote: ... cells were primed with LPS (1 µg/mL) for 4 h and treated with UCN-01 (10 µM, Sigma-Aldrich), nigericin (10 µM ...
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bioRxiv - Neuroscience 2021Quote: Mice were anesthetized with ketamine/xylazine I.P and transcardially perfused with 4% paraformaldehyde in PBS (Formalin solution, neutral buffer 10%, Sigma-Aldrich). Brains were dissected and post-fixed for 24h in 4% PFA ...
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bioRxiv - Immunology 2022Quote: ... Lysates were cleared by centrifugation at 16000g for 10 minutes at 4°C and 70µl were recovered directly in Sample Buffer 2X (Millipore Sigma) as input ...
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bioRxiv - Cancer Biology 2022Quote: ... Three 0.2 mL fractions containing EV were collected and subsequently concentrated using an Amicon Ultra-4 10 kDa filter (Millipore UFC801024).
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bioRxiv - Microbiology 2022Quote: ... Passthrough was collected and buffer-exchanged by spin concentration using Amicon Ultra-15 10-kDa 4-mL spin concentrators (Millipore).
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: Cells were fixed with 4% paraformaldehyde for 10 min and permeabilized for 40 min using 0.1% Triton X-100 (Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.4 mM dNTPs mix, 10 x primer master mix (1.6 µM FP / RP, 0.8 µM IF / IB) (Sigma, HPLC purifed), 1 x EvaGreen dye (Biotium) ...
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bioRxiv - Microbiology 2022Quote: ... MO, USA], yeast extract 4 g.L-1 [Sigma-Aldrich, St. Louis, MO, USA], malt extract 10 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Bioengineering 2022Quote: ... a buffer/chromophore reagent was prepared by mixing an equal volume (3.5 mL) of 4-aminoantipyrine (10 mM, 06800, Sigma–Aldrich) and N-ethyl-N-sulfopropyl-m-toludine (10 mM ...
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bioRxiv - Cancer Biology 2024Quote: Each sample was then tagmented by mixing 2 μL of 0.5 ng/μL cDNA, 4 μL of 2X TD buffer (20 mM Tris pH 8.0, 10 mM MgCl2, 20% dimethylformamide (Sigma, D4551)) ...