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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... a buffer/chromophore reagent was prepared by mixing an equal volume (3.5 mL) of 4-aminoantipyrine (10 mM, 06800, Sigma–Aldrich) and N-ethyl-N-sulfopropyl-m-toludine (10 mM ...
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bioRxiv - Cell Biology 2024Quote: ... washed three times with 200 μl of PBS and centrifuged at 1811 g in a pre-cooled centrifuge at 10°C prior to fixation in 100 μL 4% PFA (Sigma) for 45 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The antibody solution was then concentrated on Amicon Ultra-4 centrifugal filters with 10 or 50 kDa molecular weight cutoff (Millipore) to 1-4 mg/ml in PBS.
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cultures were transduced with concentrated lentivirus at an MOI of 10 for 48 hours in 4 µg mL−1 polybrene (Sigma), then selected in normal growth media supplemented with appropriate antibody selection ...
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bioRxiv - Developmental Biology 2024Quote: ... All mice were anesthetized with 4% isoflurane and intracardially perfused with 30 mL of 0.1 M PBS containing 10 U/ml heparin (Sigma, H3149) and 2 mM ProHance (a Gadolinium contrast agent ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates were dialysed back into Buffer A overnight at 4 °C prior to concentration with 10 kDa MWCO Amicon centrifugal filters (Millipore, Sigma), quantification by absorbance at 280 nm ...
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bioRxiv - Developmental Biology 2023Quote: ... were excised from the surrounding tissue and immediately fixed in a tension-free state using 10% formalin (for SHG) or 4% para-formaldehyde (for SEM) (Sigma), and stored at 4 °C.
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bioRxiv - Neuroscience 2023Quote: ... The beads were washed once with 1 mL of isolation buffer and then incubated overnight at 4°C with 10 μg of either anti-CD9 (clone CBL162, Millipore), anti-L1CAM (clone 5G3 ...
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bioRxiv - Immunology 2023Quote: ... TIGIT-/- and TIGIT-/-ICOSL-/- NOD mice were incubated 4 hours at 37°C with Phorbol 12-myristate 13-acetate (PMA 10 ng/ml; Sigma), ionomycin (500 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: Each sample was then tagmented by mixing 2 μL of 0.5 ng/μL cDNA, 4 μL of 2X TD buffer (20 mM Tris pH 8.0, 10 mM MgCl2, 20% dimethylformamide (Sigma, D4551)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was further centrifuged at 17,684× g for 90 mins at 4 °C and the pellet obtained was resuspended in TE-SDS (10 mM Trizma base (pH 8.0) (Sigma-Aldrich), 1 mM EDTA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The abdominal cavity was opened and the animal was transcardially perfused with ∼10 ml 0.9% NaCl in ddH2O followed by 20 mL 4% paraformaldehyde (#158127; Millipore Sigma) in 0.1M phosphate buffer (PB ...
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bioRxiv - Immunology 2022Quote: ... Lysates were cleared by centrifugation at 16000g for 10 minutes at 4°C and 70µl were recovered directly in Sample Buffer 2X (Millipore Sigma) as input ...
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bioRxiv - Cancer Biology 2022Quote: ... Three 0.2 mL fractions containing EV were collected and subsequently concentrated using an Amicon Ultra-4 10 kDa filter (Millipore UFC801024).
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bioRxiv - Microbiology 2022Quote: ... Passthrough was collected and buffer-exchanged by spin concentration using Amicon Ultra-15 10-kDa 4-mL spin concentrators (Millipore).
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: Cells were fixed with 4% paraformaldehyde for 10 min and permeabilized for 40 min using 0.1% Triton X-100 (Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... incubating them in 0.5 ml cFAD with 3.75×10^7 PFU/ml supplemented with 4 μg/ml Polybrene (Sigma Aldrich, H9268) on top of the insert and 2 ml cFAD on the bottom ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed at room temperature for 10 min in PBS with 4% (v/v) formaldehyde and blocked with 100 μg/mL DNase-free RNase A (Sigma) in antibody dilution buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Right tibias from SMAD1/5WT and SMAD1/5iΔEC mice (n= 10 per group) were collected and fixed in 4% paraformaldehyde (PFA; Sigma Aldrich) in PBS overnight (12h ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected after centrifuging at 4°C for 10 minutes and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD ...
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bioRxiv - Physiology 2023Quote: Cells were boiled for 10 min in lysis buffer (4% SDS, 50 mM Tris-HCl, pH 8.5) supplemented with 50 mM iodoacetamide (Sigma-Aldrich) to block reduced Cys residues71 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were fixed with 4% paraformaldehyde (nacalai tesque) for 10 min at room temperature followed by permeabilization with 0.2% Triton X-100 (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma] ...
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bioRxiv - Genomics 2024Quote: ... the coverslips were mounted on glass slides using an antifade mounting media (10% w/v Mowiol 4-88(EMD Millipore)/1% w/v DABCO (Sigma-Aldrich)/25% glycerol/0.1 M Tris ...
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bioRxiv - Immunology 2024Quote: ... Derived monolayers were subsequently differentiated for 4 additional days in intestinal growth media to which 10 µM DAPT (Sigma, D5942) and 1 µM PD0325901 (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... of ∼0.4-0.8 were washed 5 times either at room temperature or at 4°C in sterile aqueous solution containing 10% glycerol and 0.05% Tyloxapol (Sigma; #T8761). At each step the volume washing solution was decreased (e.g 50 mL ...
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bioRxiv - Microbiology 2024Quote: iBMDMs expressing eGFP-VAMP2 were plated at 4×10⁴ cells per well in an 8-well Millicell EZ slide (Sigma) then infected with mCherry-expressing M ...
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bioRxiv - Microbiology 2024Quote: Differentiated THP-1 cells were plated at 4×10⁴ cells per well in an 8-well Millicell EZ slide (Sigma) and then infected with mEmerald-expressing M ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Genomics 2024Quote: ... in 270 μl (Table 4) CD buffer (15 mM NaCl, 10 mM Tris-HCl) with and without hexaaminecobalt(III) chloride (1 mM) (Sigma). DNA probes were incubated at 37 °C for 2 hours to allow a potential B-Z transition ...
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bioRxiv - Bioengineering 2024Quote: Cells were fixed overnight with 4% formaldehyde for 10 minutes and then permeabilized at room temperature with 0.5% (v/v) Triton X-100 (SIGMA-ALDRICH) in PBS for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... YFP-Rab2BS21N or YFP-Rab2BQ66L in PCF cells was induced by 10 µg/ml cumate (4-Isopropylbenzoic acid, Sigma, 268402) for 24-48 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1M NaCl, 10 mM EDTA, 50 mM L-Ascorbic acid, 0.2 M disodium tartrate, 82 mM 4-nitrolphenylphosphate, all Sigma-Aldrich) added prior to incubation in the dark for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 1 hour at 4°C with 10% (w/v) trichloroacetic acid (TCA) solution (Sigma-Aldrich, T6399). Wells were washed with water and left to dry at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... EV-enriched fractions (1-4) were pooled and concentrated using Amicon 15 Ultra RC 10 kDa MWCO filters (Merck Millipore). Expi293F-palmGRET EV aliquots in Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Neuroscience 2022Quote: ... and then plated into 24-well (1.8 x 10 5 cells per well) plates coated with 50 μg/ml poly-d-lysine (Sigma). After removal of the coating solution ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... and added to the cells for 5 min without and for another 40 min with 10 mM H2O2 (Sigma). After washes in CB ...
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bioRxiv - Biochemistry 2020Quote: ... All of these chemicals were prepared as 10 mM stocks in DMSO (CAS: 67-68-5) purchased from Sigma and further diluted in DMSO to the desirable concentrations ...
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bioRxiv - Biophysics 2021Quote: ... was grown in tryptone broth (10 g I−1 Tryptone, microbiologically tested, Sigma Aldrich, 5 g I−1 NaCl) in an orbital shaker overnight at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Separation was performed using a reversed phase column (25 cm L x 10 mm D, 5 µm, Sigma-Aldrich) controlled by the LabSolutions software ...
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bioRxiv - Plant Biology 2021Quote: ... indica growth in axenic culture, five different concentrations (5, 10, 20, 50 and 100 μM) of putrescine (Sigma®) was directly supplemented to fungal defined minimal medium (MN medium ...
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bioRxiv - Microbiology 2021Quote: ... The culture was then plated on SA (1% glucose) agar plates supplemented with 10 μg/mL 5-Fluoroorotate (Sigma). Plates were incubated at 30 °C until colonies emerge ...
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bioRxiv - Systems Biology 2020Quote: ... Both solutions were composed of a CTM buffer supplemented with 10 mM Levamisole (Sigma, CAS Number: 16595-80-5). Levamisole was used to minimize worms’ movements in the microfluidic device during imaging ...