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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Intact and well-shaped slices (approximately 5-10 mm diameter) were then placed on top of a porous membrane insert (0.4 µm, Millipore). Then the membrane inserts were placed into 6-well plates containing 1.5 mL maintenance medium consisting of F12/DMEM (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were digested in 10 mL Collagenase P 5 U/mL (11 213 873 001, Sigma Aldrich, Buchs, Switzerland) for 40 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... in 1:1 DMEM/Ham’s F12 and varying concentrations (i.e. 0, 2.5, 5, 10, or 20 nM) of epidermal growth factor (EGF) (Sigma Aldrich). Each of these groups were done in triplicate wells ...
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bioRxiv - Bioengineering 2022Quote: ... Harvested lung samples at 5 dpi were fixed in 10% formalin neutralization buffer (Sigma-Aldrich, Saint Louis, MO, USA) and further processed for histopathological examination using hematoxylin- and-eosin (H&E ...
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO], Dispase 5 mg/ml [GIBCO], Y-27632 10.5 µM [Sigma], DNase I 10 µg/ml [Sigma] ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen sections were rinsed with PBS 1X for 10 min and blocked with 5% normal goat serum (NGS) and 0.3% Triton X-100 (Sigma) in PBS 1X for 1hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Mouse model of cytokine storm was induced by intraperitoneal (i.p.) injection with LPS (5 or 10 mg/kg, in 100 μl PBS, Sigma). For EV treatments ...
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bioRxiv - Molecular Biology 2020Quote: ... a 5 mL starter culture was grown in Luria-Bertani liquid growth media (LB, containing of10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.0, Sigma) at 25°C overnight ...
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bioRxiv - Genetics 2022Quote: ... followed by strong douncing in cell lysis buffer buffer (KCL 85 mM, HEPES 5 mM, NP-40 0.5%, Sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma) following by 5 min centrifugation at 2000g ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μg of plasmid DNA was transfected per plate by incubating the DNA with 10 μg polyethyleneimine (Sigma, 408727) in serum free media for 10 min before being added dropwise to cells that had been changed to serum free media 1 hour prior.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were permeabilized with 0.1% Triton X-100 (Integra) for 10 minutes and blocked with 5% goat serum (Sigma) in PBS-Tween (0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1.47 mM KH2PO4 supplemented with 5 mM DTT and concentrated to ∼10-20 mg/mL using 3 kDa Millipore concentrator and stored at -80 °C.
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bioRxiv - Genetics 2020Quote: ... nematodes were cultured and then assayed for survival on plates with 10 μg/ml 5-fluoro-2-deoxyuridine (Sigma), to avoid vulval rupture [27] and eliminate live progeny ...
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bioRxiv - Neuroscience 2020Quote: ... 10% methanol in PBS-T 0.1% for 30mins and then in blocking solution containing 5% goat serum (Sigma Aldrich), 5% BSA (Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Tubes were pre-warmed to 37°C for 5 minutes before incubation with 10 U/ml MNase (Sigma, #N3755) for 15 minutes at 37°C under spinning at 800 rpm in an Eppendorf Thermomixer comfort ...
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bioRxiv - Neuroscience 2021Quote: ... the dopamine precursor L-Dopa + benserazide (10/5 and 50/25 mg/kg; Sigma Aldrich D1507 and B7283, respectively), the D2 agonist quinpirole (0,5 mg/Kg Sigma Aldrich Q111 ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl blocking buffer (10 mg/ml mouse IgG with 5 mM Azide) and 0.5 μl 0.5% Phenol red (Sigma P0290) were added for blocking the unconjugated Anti-Mouse-IgG-Atto647N and vortexed thoroughly ...
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bioRxiv - Biochemistry 2021Quote: ... COS-7 and HEK 293T cells were grown at 37 °C in a 5% CO2 atmosphere in cell culture medium (Dulbecco’s modified Eaglès medium (CA 10-013, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Treatments with ATR inhibitor VE-821 (5 μM, Bioconnect, #S8007) and ATM inhibitor KU-55933 (10 μM, Sigma, #SML1109) were started 2 hours before 5-FU administration ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed with PBS twice and treated with 10 μM of 5-aza (Sigma, St. Louis, USA) in DMEM-F2 medium (DMEM-F2 ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher) supplemented with 10% tetracycline-tested FBS (Sigma), 100 μg/mL Zeocin (Thermo-Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.5 μM (with apoptosome at a molar ratio of 1:5:10 caspase-9 : Apaf-1 : cytochrome C (SIGMA)) in the following buffer conditions ...
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bioRxiv - Biochemistry 2022Quote: ... The protein solution was concentrated to 5 mL in a 10 kDa molecular weight cutoff centrifugal ultrafiltration device (Millipore) and loaded onto a Sephacryl S100 HR 26/60 size-exclusion chromatography column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 105 to 1 × 106 cells/ml were resuspended in freezing media (FBS + 10% Dimethyl Sulfoxide, DMSO, Sigma, D2660) and frozen down.
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bioRxiv - Cell Biology 2022Quote: ... in PBS for 10 min and subsequently blocked in 5% (w/v) BSA (Sigma-Aldrich, St Louis, MO, USA) with 0.03% Triton-X in PBS for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed once in PBS + 5 μM Actinomycin D and filtered through a 10 μm strainer (Pluriselect) in 60 μl PBS + 0.04% BSA (Sigma).
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bioRxiv - Immunology 2022Quote: ... supplemented with 5% defibrinated sheep blood (Hemostat, Dixon, CA) and 10 ug/ml neomycin (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Physiology 2022Quote: ... Previously snap-frozen muscle fibers (∼5-10 mg) were pulverized using 25 ml ice cold CelLyticMT (Millipore Sigma C3228) containing 0.2% protease inhibitors (Millipore Sigma P8340 ...
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 6% rabbit serum (Pel-Freez, 31125-5) and 10 ug/mL gentamicin (Sigma-Aldrich, 1405-41-0) to maintain GFP-tagged B ...
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bioRxiv - Molecular Biology 2022Quote: ... and eluted with 5 CV of elution buffer (10 mM reduced glutathione [Sigma], 50 mM Tris-HCl, pH 9.0). Protein purity was determined via SDS–PAGE (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by strong douncing in cell lysis buffer (KCL 85 mM, HEPES 5 mM, NP-40 0.5%, Sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma) following by 5 min centrifugation at 2000g ...
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bioRxiv - Molecular Biology 2024Quote: ... for 10 min at room temperature (RT) and permeabilized for 5 min in 0.5% Triton X-100 (#T9284, Sigma) with 2 mM VRC diluted in PBS on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in 4-well plates containing 500 μl of MEM-C supplemented with ITS (10 μg/mL insulin, 5.5 μg/mL transferrin and 5 ng/mL selenium; Sigma), FSH (10 mIU/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were flushed from the oviduct (E3.5) or uterine horns (E4-E5.75) using 5-10 ml of warm M2 medium (MR-015-D, Sigma Aldrich) supplemented with 1 % Penicillin-Streptomycin (15130122 ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µgs of caput and cauda amyloid fractions were incubated in a final volume of 40 µl containing dPBS and 1 µl (12.5 μg/ml final) DNase-free RNase in RNase buffer (10 mM Tris, pH 7.4, 5 mM CaCl2, 50% glycerol) (Sigma) or RNase buffer alone at 37°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... the calcium signal was stimulated with a micropipette containing 10 μmol/L 5’-ATP-Na2 solutions (Sigma-Aldrich, A1852)51.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for 5 days in organoid feeding medium containing 10 or 25 nM of Gemcitabine (Sigma, #G6423) or Erlotinib (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... pooled and filtered through a 0.22-μm filter before ion exchange purification on a MonoQ column (10 mL, 5/50 GL; Sigma) that was pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... indole and 7-cyanoindole solutions were prepared at 5 mg/ml in vehicle (10% DMSO and Millipore-filtered water), aliquoted and stored at −20 °C ...
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bioRxiv - Genomics 2023Quote: ... The second barrel was filled with 5 μl of the organic phase mixture of 10 mM THATPBCl in 1,2 DCE (Sigma), following silanisation of the backend of the nanopipette by exposure to Trichloro(1H ...
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bioRxiv - Neuroscience 2023Quote: ... A solution of 10 mM 5-fluoro-2’-deoxyuridine and uridine (anti-mitotic agent) (Sigma Aldrich, St. Louis, USA) was added to the cultures at days in vitro (DIV ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking for 10 min with 5% nonfat dried milk in PBS with 0.05% Tween-20 (Sigma-Aldrich, P1379) (PBST) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20-50μL concentrated (or equivalent non-concentrated) viral supernatant(s) were added to 5-10×104 suspended cells supplemented with 10μg/mL polybrene (Sigma), then transferred into a 12-well plate for 2-3 days before changing to fresh media ...
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bioRxiv - Neuroscience 2023Quote: ... cells were permeabilised with 0.1% Triton X-100 for 10 minutes and blocked with 5% horse serum (Sigma, H0146) for 1 hour ...