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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from groups of 4-5 aphids of the above-described strains using TRI Reagent® (T9424, Sigma-Aldrich, USA) and Direct-Zol RNA extraction Kit (R2052 ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were perfused first for 5 min with NaCl 0.9% and then for 15 min with 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO) in sodium phosphate buffer (PB 0.1 M ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions were incubated for 4 hours at 37°C and 5% CO2 in supplemented RPMI 1640 media supplemented with PMA (50ng/mL, Sigma Aldrich, P8139-1MG), ionomycin (750ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the following primary antibodies diluted in 5% TBST: GluA1-C (Millipore, Cat# AB1504, 1:1000), GluA2-C (NeuroMab ...
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bioRxiv - Biochemistry 2024Quote: ... Ireland) for 5 min at 4 °C using a Centricon 50 kDa molecular weight cut off (MWCO) concentrator (Millipore: MA, USA, Lot R0KA67379). After the 5 min spin ...
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bioRxiv - Plant Biology 2024Quote: ... medium lacking Trp but containing 40 μg mL-1 5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside (X-α-gal; Sigma‒Aldrich) at 30°C for 3 days ...
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bioRxiv - Microbiology 2024Quote: ... Membrane protein topology was assayed by plating the resulting reporter strains on dual-indicator plates containing LB agar supplemented with 80 μg/ml 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) ([Sigma Aldrich], RES1364C-A101X) and 100 μg/mL 6-chloro-3-indolyl-β-d-galactoside (Red-Gal ...
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bioRxiv - Biochemistry 2024Quote: ... Bands were visualized using the nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (BCIP/ NBT, Sigma-Aldrich, St. Louis, MO, USA) reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... oral gavage for 21 days, using a 4-day-on, 3-day-off schedule) (Selleckchem, S7694), tamoxifen (20mg/kg, subQ, 5 doses/week) (Sigma Aldrich, T5648-1G), abemaciclib (75mg/kg ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 h in 1% osmium tetroxide in H2O and dehydration in an ethanol gradient (30%, 60%, 90%, 4×100% for 5 min each) and chemical drying in hexamethyldisilazane twice for 2 min (Sigma-Aldrich, Oslo, Norway). Before imaging samples were mounted on aluminum stubs and coated with 10 nm layer of gold/palladium alloys.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were incubated for 2h at 4°C with 100 ng of the different GST-V5-CD95 constructs and then overnight at 4°C with 10 µl of Anti-V5 Agarose Beads (Sigma-Aldrich). After extensive washing in Hepes buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to a 4% agar pad with 10 mM sodium azide and 1 mM levamisole in M9 solution (31742-250MG, Sigma-Aldrich) on a slide for imaging ...
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bioRxiv - Microbiology 2020Quote: ... Zirconia beads were pelleted by centrifugation at 21,000xg for 10 min at 4° C and supernatant was transferred to an RNase-free tube containing 100 μL chloroform (Sigma Aldrich). Samples were vortexed for 15 s prior to a 10 min RT° C incubation ...
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bioRxiv - Cell Biology 2021Quote: ... AURKA antigen retrieval was performed by incubating cells previously fixed in 4% PFA/0.2% Glutaraldehyde in a 10 mM sodium citrate tribasic dihydrate solution (Sigma-Aldrich, C8532), pH 6 at 98°C on a hot plate for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody reaction was carried out for 10 days at 4°C in blocking solution (1:1000 mouse monoclonal Tyrosine Hydroxylase antibody; T2928, Sigma-Aldrich). Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were spun for 15 min at 515g at 4°C and the pellets were resuspended in 1 ml cold freezing medium (10% DMSO (Sigma, D2438) in FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial organoids were pelleted by centrifugation at 1500 rpm for 10 minutes and re-suspended in 4 ml of DMEM/F12 with 40 μl of DNase (Sigma, #5025) (2 U/μl) ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Genomics 2021Quote: ... cells were switched back to FluoroBrite DMEM with 10% FBS and GlutaMAX and treated with 4 mM freshly prepared hydroxyurea (Sigma H8627) and 100 μM abscisic acid (Sigma A1049) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Tissues were then minced and shaken at 37°C for 20 min in digestion buffer (HBSS with calcium and magnesium, supplemented with 10 mM HEPES, 4% FBS, penicillin-streptomycin (Sigma Aldrich), 0.5 mg/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated in 500 ng/ml 4’,6-diamidino-2-phenylindole (DAPI, diluted in 0.1 M PB, Sigma, 10 min) prior to mounting using Prolong Gold (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... the gels were washed 3 x 20 min with 3.5 ml of ultrapure water and selected specimens were also incubated for 30 min at room temperature with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, D9542) or 10 µg/ml Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Neuroscience 2020Quote: ... Equal concentrations of serially diluted lysate (in the range of 0-60 μg/ml) from control and Thap1 cKO genotypes was incubated with 10 mM 4-methylumbelliferyl β-D-glucuronide (MUG, Sigma) in 0.2M Sodium acetate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... RCECs were fixed with 4% PFA for 15 minutes and stained with crystal violet for 10 minutes (Sigma, St. Louis, MO) and counted using a light microscope.
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bioRxiv - Neuroscience 2020Quote: ... Then slices were washed at least 4×10 min in PBS and mounted on the glass slide using moviol (Sigma-Aldrich). Imaging was done using a Zeiss LSM710 Multiphone microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde for 10 minutes and stained with 0.5% w/v crystal violet solution (Sigma-Aldrich, UK) for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... A 2 μL aliquot of 100 μM aptamer in PBS was thawed and mixed with 4 μL of 10 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) for 8 hours to reduce the disulfide bond ...
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bioRxiv - Cell Biology 2022Quote: ... fixed with 4% formaldehyde (SAV Liquid Production) for 10 min at RT and permeabilized with 0.1% Triton™ X-100 (Sigma Aldrich) in PBS for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... single cell suspensions from tumor tissue were resuspended in RPMI media supplemented with 10% FBS and 100 IU/ml P/S and incubated for 4 hours with PMA (20 ng/ml, Sigma-Aldrich), Ionomycin (1 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: Cells grown on coverslips were briefly washed with PBS once before fixing for 10 min at room temperature using 4% paraformaldehyde (Sigma Aldrich) and 4% sucrose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Insoluble debris was removed by centrifugation at 15,000 rcf for 30 min at 4°C following which 2.1 ml of neutralized 10% polyethyleneimine (Millipore Sigma, cat#P3143) was added to the decanted supernatant dropwise while stirring at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were reduced with 4 mM DTT for 30 min at 25°C and alkylated by 10 mM iodoacetamide (Sigma-Aldrich) for 45 min in dark in a thermoshaker ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2020Quote: ... resuspended in 4°C cryopreservation medium consisting 90% fetal bovine serum (FBS, HYCLONE, Cat. sh30084.03) and 10% Dimethyl sulfoxide (DMSO, Sigma, Cat. D4540) and then placed in Mr ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Bioengineering 2021Quote: BMOs were collected from the array as described above and fixed with 2 % or 4 % PFA or 10 % Formalin (Sigma, HT501128) overnight at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... 2 mM glutamax and 20 μg/mL gentamicin on the surface coated with 10 μg/ml PDL and 4 μg/ml Laminin (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were spun down at 4,000 x g at 4°C for 10 minutes before being resuspended in PBS containing 1X Bugbuster lysis reagent (Millipore Sigma). Suspensions were incubated on ice for 30 minutes before being spun down at 15,000 x g at 4°C for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Immunofluorescence was performed on adhered cells after fixation with 4% PFA at room temperature (RT) for 10 min and permeabilization with 0.5% Triton® X-100 (Sigma-Aldrich) at RT for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, 10 μg/ml, Cat.No.: D27802, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and were then transferred to formaldehyde solution (4% paraformaldehyde, P6148, Sigma-Aldrich in 1X PBS + 10% Triton-X, X100, Sigma-Aldrich) and incubated for 20-30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved in SDS-polyacrylamide gels (4 % stacking and 10 % running) and transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon-P, EMD Millipore), according to standard protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μm tissue sections (2-4 sections per donor) were fixed with 10% neutral buffered formalin solution (catalog no. HT501128, Sigma-Aldrich) for 30 min at room temperature ...