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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2 ug mL-1 N-(6-(isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine hydrochloride (GSK’872) (Sigma-Aldrich # 5303890001) and 2 ug mL-1 Necrosulfanamide (EMD Millipore #480073 ...
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bioRxiv - Systems Biology 2024Quote: Differentiated APCs were fixed in 4% PFA for 1 hour at room temperature and lipid droplets were stained with Oil Red O (Sigma-Aldrich). APCs were lysed with lysis buffer (1:25 IGEPAL in 100% isopropanol ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were fixed using 4% paraformaldehyde and neutral lipids were stained using 1:500 5 mM Bodipy 493/503 (Sigma, #D3922) while nuclei were stained using 1:1500 Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin, F1141, Sigma-Aldrich) tissue cultureware.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and 15 mL/L of a 10% methyl-4-hydroxybenzoate solution in ethanol) supplemented with 0.5% erioglaucine dye (brilliant blue R, FD&C Blue No.1, Sigma-Aldrich, #861146) and allowed to feed for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... to 9 mL of 1× phosphate-buffered saline with 4 µL of Protector RNase inhibitor (Sigma-Aldrich, St. Louis, MO, USA). Eight (8 ...
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Cell Biology 2024Quote: The samples were fixed in 4% PFA for 1 h at room temperature (RT) and then incubated with 15% sucrose solution (Sigma Aldrich) supplemented with 0.03% eosin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were separated on 10% PAA gel and incubated overnight at 4 °C with anti-Tubulin (Sigma, #T6074, dilution 1:10,000). HRP-conjugated anti-mouse IgG binding protein (Santa-Cruz ...
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bioRxiv - Microbiology 2024Quote: ... placed in digestion media containing 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Homogenate was subjected to high speed centrifugation (100 000g, 1 h, 4 °C) and supernatant was neutralized with 0.5M Tris-HCl (pH 6.8) (Sigma, St.Louis, MO, USA). Aβ1-40 levels in brain and plasma samples were quantified by ELISA using Meso Scale Discovery (MSD ...
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bioRxiv - Neuroscience 2024Quote: ... were recorded in voltage clamp configuration at –70 mV in presence of 1 µM TTX (Alomone T-550) and 100 μM 4-AP (Sigma 275875). Drugs were washed in at least 10 min before starting recordings ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked at room temperature for 1 hour in 4% normal serum with 0.1% Triton X-100 (Millipore Sigma; T8787). Sections were incubated in primary antibody in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... or primary antibody overnight at 4° C followed (in the latter case) by a 1-hour incubation with protein-A agarose beads (Sigma #16266) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were diluted in Pierce Protein-free blocking buffer and incubated overnight at 4°C: Anti-RIG-I (1:500, clone 1C3, MABF297 Sigma-Aldrich), anti-dsRNA J2 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 uL of cell lysate was incubated with 1 mM of 4-methylumbelliferyl 6-sulfo-N-acetyl-b-D-glucosaminide (Sigma, 454428) in 10 mM citrate phosphate buffer with 0.5% Triton-X-100 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293F GnTI-cells were transfected with a total of 1 mg plasmid DNA (550 μg RagA, and 450 μg RagC) and 4 mg polyethylenimine (PEI) (Sigma-Aldrich) per liter at a density of 1.8 × 10^6 cells/ml ...
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bioRxiv - Bioengineering 2024Quote: ... poly(sodium-4-styrenesulfonate) (PSS, Cat No. 25704-18-1, 70 kDa), and tetrahydrofuran (THF, Cat No. 401757) were purchased from Sigma-Aldrich, Inc. ...
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bioRxiv - Biophysics 2024Quote: ... the cells were treated with a range of doses (100, 33.3, 10, 3.3, 1, 0.3, 0.1, 0.03 µM) of nirmatrelvir (Sigma; 4 mM stock solution in DMSO) and BRET measurements were taken after 22 h by adding furimazine (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... gels were incubated in primary antibodies (Supplementary Table 4) diluted to 1:500 in antibody dilution buffer (2% bovine serum albumin (BSA; Sigma, A9647) in 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... main stem bronchi and 2-3 further branching generations pf the cartilaginous airways were dissected into sections between 1-4 cm2 in size and placed in a solution (%w/v) of 0.1% Protease XIV (Sigma Aldrich, #P5147) and 0.001% DNase (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 mg plasmid DNA (200 μg each of the five constructs) and 4 mg polyethylenimine (PEI) (Sigma-Aldrich) were used to transfect HEK293F GnTI-cells at a density of 1.8 × 10^6 cells/ml per liter ...
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bioRxiv - Cell Biology 2024Quote: ... The bacteria were kept at 4°C for 10 min before supplementing the LB with 0.5 mM isopropyl ϕ3-d-1-thiogalactopyranoside IPTG (Sigma, I5502-10G) to induce TXNL1 expression ...
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bioRxiv - Cell Biology 2024Quote: ... CL4B resins were pelleted at 16,900 g and 4 °C for 1 min and the supernatants were then incubated with 200 µl M2 magnetic beads (Sigma M8823) and immunoprecipitated at 4 °C for 16h ...
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bioRxiv - Cell Biology 2024Quote: ... Viral supernatants were filtered and used to infect cells by spin-infection (2500 rpm centrifugation at 25 °C for 1 h) in the presence of 4 µg/mL polybrene (Cat#S2667, Sigma-Aldrich). Transduced cells were enriched either by antibiotic selection or expression of fluorescent protein using a BD FACSAria Fusion cell sorter.
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bioRxiv - Developmental Biology 2024Quote: ... and then stained overnight at 4°C with antibody mix diluted in PBS-1%BSA (BSA Cohn fraction 30%, Sigma-Aldrich). After three 8 minutes washing steps (Triton X-100 diluted at 0.2% within dPBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then incubated overnight at 4° C in primary antibody solution: mouse anti-acetylated Tubulin (1:500; Sigma catalog # T7451) and mouse anti-Islet-1 40.2D6 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... 1:100; anti-calbindin, CB38, Swant, 1:5000; anti-CT-B, #703, List Biological, 1:4000; anti-VGLUT1, AB5905, Millipore, 1:5000) were diluted in PBS-TX with 3% normal serum and sections were incubated in this cocktail for 48-60 hours at 4 ºC ...
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bioRxiv - Biochemistry 2021Quote: ... Additionally glycans were co-incubated with 1 mg ml−1 XynD and 1 mg ml−1 α-Mannosidase from Canavalia en-siformis (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... 1:500), Rabbit anti-phospho Chk1 (CST, 1:200), Rabbit anti-phospho Smad (CST, 1:150) Rabbit anti-pH3 (Millipore, 1:500), and Alexa 488/568/647-conjugated Donkey anti-Chicken/Rabbit/Mouse secondary antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000; IMPDH2, Abcam ab129165, 1:1000; S6, Santa Cruz sc74459, 1:1000; p-S6, Cell signaling 5364, 1:1500; Tubulin, Sigma T6074 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-TRPC7 (1:500, 1% casein, #SAB5200051, Sigma-Aldrich), anti-PKC (1:250 ...
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bioRxiv - Cancer Biology 2020Quote: ... and active caspase-3 (1:1 000, Millipore, AB3623). Three fields of view for each slide were imaged using an Olympus BX43 Upright Microscope and cells were manually counted and scored using ImageJ Software.
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bioRxiv - Developmental Biology 2021Quote: ... 7-AAD (Sigma, 10 µg ml−1, 1:100) or Zombie Aqua (Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... CDM (IMDM + Ham’s F-12 at 1:1 (Sigma), chemically defined lipid concentrate (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... Puromycin (cat# MABE343, Merck Millipore; 1:2,000-1:10,000), anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263 ...
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bioRxiv - Microbiology 2020Quote: ... 100 nM 1-Aminocyclopropane-1-carboxylic acid (ACC, Sigma), 100 nM 2,4-Dichlorophenoxyacetic acid (2,4-d ...
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bioRxiv - Immunology 2021Quote: ... 1 ml of 1% low-melt agarose (Sigma Aldrich) was gently infused ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, rabbit, Dako; 1:1000, chicken, Millipore), MBP (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Penicillin/Streptomycin and 1% L-Glutamine (Sigma, UK). After 1 week in culture cells were transduced at various MOIs with LV-LTα ...
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bioRxiv - Biochemistry 2021Quote: ... 1× PBS and 1× protease inhibitor cocktail (Sigma-Aldrich) and probe tip sonicated for three cycles for 20 s and intervals of 30 s on ice 22 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 g/ml heparin and ITS+1 supplement (Sigma), referred to as “defined medium” ...
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bioRxiv - Cancer Biology 2020Quote: ... or plakophilin-1 (Sigma-Aldrich, catalog #HPA027221, 1:300) in antibody dilution buffer (catalog #12378 ...
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bioRxiv - Cancer Biology 2020Quote: ... G6 (1:500) and tubulin (T6557, Sigma, 1:3000). Antibodies used for immunofluorescence staining were ...
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bioRxiv - Cancer Biology 2020Quote: ... G6 (1:250) and tubulin (6074, 1:250, Sigma). Fluorescently labeled phalloidin (Alexa Fluor-350 ...
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bioRxiv - Systems Biology 2021Quote: ... 1 μM Mek 1/2 inhibitor PD0325901 (Sigma, PZ0162), 0.1 mM LIF ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL fluorescein solution (1 μg/ mL, Sigma, USA) was simultaneously added into the center of the agar plate.
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bioRxiv - Neuroscience 2020Quote: ... panel 1 = anti-mouse Neun (AB90, Millipore, 1:500), anti-mouse Iba1 (019-19741 ...
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bioRxiv - Neuroscience 2020Quote: ... or mHTT protein (1-82aa, Millipore, MAB5492, 1:500), and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories ...