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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... larvae were incubated overnight at 4°C in the following primary antibodies: mouse α-FLAG antibody (1:1000; Sigma, cat# F3165, RRID:AB_259529); rabbit α-RFP (1:500 ...
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bioRxiv - Immunology 2021Quote: ... The membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-PALS-5 diluted 1:1,000 and mouse anti-tubulin (Sigma, catalog number T9026) diluted 1:3000 in blocking buffer) ...
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bioRxiv - Genetics 2021Quote: ... pH 7.4) before being incubated with primary antibodies (Anti-Telo-Collagen Type I, A1, ABT256, Sigma-Aldrich; Anti-Fibronectin-1, F3648, Sigma-Aldrich) followed by anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclei were lysed for 1 h at 4 °C in a presence of 150 U of Benzonase nuclease (Novagen, cat. no. 71206) while rotating ...
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bioRxiv - Cell Biology 2022Quote: ... cleared cell lysates were incubated rotating for 1 hr at 4°C with 20 μl bead slurry of Anti-FLAG (M2) conjugated beads (Sigma, cat# A2220) or 25 μl bead slurry of GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then fixed for 10 min at room temperature with freshly prepared 1% formaldehyde in PBS (Sigma-Aldrich, F8775-4×25ML) and the reaction then quenched by addition of 0.2M glycine (NZYTech ...
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bioRxiv - Neuroscience 2020Quote: ... animals were deeply anesthetized by intraperitoneal injection of 1 ml Vetbutal and intracardially perfused with 4% paraformaldehyde (Sigma-Adrich; St.Louis, MO, USA). The brains were removed and cortices of separated hemispheres were flattened between glass slides to 3 mm ...
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bioRxiv - Neuroscience 2020Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-M4 polyclonal antibody (1:200; H-175, Millipore, Burlington, USA) in PBS containing 0.1% Triton X-100 and 1% normal donkey serum for 2 days at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100 in PBS at 4°C overnight: Primary antibodies used were anti-NeuN (1:1000, Millipore, ABN90P, lot#3226535), polyclonal rabbit anti-HA (C29F4 ...
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bioRxiv - Bioengineering 2021Quote: ... The gel compartment was removed after 1 to 5 days of incubation (depending on fusion) by adding 4 w/v% sodium citrate (Sigma Aldrich Inc.) for 15 min as a lyase for alginate (Supplementary figure 5 ...
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bioRxiv - Biochemistry 2021Quote: ... and GUS activity was measured after 30 minutes of incubation in the presence of 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG, Sigma Aldrich). Reactions were stopped by the addition of four volumes of 0.2 M Na2CO3 and fluorescence was measured in a Biotek Synergy microplate reader with excitation and emission wavelengths of 365 nm and 455 nm ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated overnight at 4°C with 1:2000 dilution of primary antibodies (FLAG; Sigma, F7425 and HA; Invitrogen, 71-5500) followed by incubation with 1:5000 dilution of HRP-coupled secondary antibodies for 2h at room temperature and detected using enhanced chemiluminescence (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... Cycloheximide (CHX, C4859), dimethyl sulfoxide (DMSO, D4540,) and 1, 4-dithiothreitol (DTT, D0632) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Protease inhibitor cocktail (Mannheim ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with crystal violet (1% w/v in 20% methanol, Sigma, cat# C0775) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... from the free radioactivity by transferring the cell suspension to a 0.4 mL centrifuge tube (USA Scientific, Ocala, FL) with a layer of 1:37.5 n-dodecane:bromo-dodecane (Sigma–Aldrich, St. Louis, MO) and spinning at 12,535 x g ...
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bioRxiv - Cell Biology 2021Quote: ... K562 Cells were plated on the pre-coated coverslips and grown for 1 hr followed by fixation using 4% paraformaldehyde (Sigma Aldrich, 47608) in PBS for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated at 4°C for 24 hours with the following primary antibodies: (I) rabbit polyclonal anti-ERα antibody (1:2,000; Sigma-Aldrich, 06-935) (II ...
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bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
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bioRxiv - Cell Biology 2022Quote: 1 mg of Triton-soluble samples prepared as described in (9) were incubated with 4 μl of Cx43 antibody (Sigma C6219, rabbit) and subsequently immunoprecipitated ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated with the primary antibodies in a blocking solution for 5 days at 4°C: mouse anti-GFAP (G3893, Sigma, 1:300), mouse anti-HSP70 (MA3-028 ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) and incubated at 4°C overnight with the following antibodies: anti-FLAG (Sigma, F1804; 1:1,000), anti-SLC1A3 (Genetex ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... and then nuclei were simultaneously incubated with the three fluorescently-conjugated primary antibodies (0.1% BSA/1X PBS) for one hour at 4°C on rotation: mouse anti-NEUN-AF555 (Millipore, MAB377A5, 1:1000); mouse anti-PAX6-APC (Novus Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... fixed overnight (ON) in paraformaldehyde 4% and stored at -20 °C in cryoprotectant (150 g of sucrose (Sigma - CAS 57-50-1) and 150 mL of ethylene glycol (Sigma - CAS 107-21-1 ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 1% BSA in PBS for one hour and then incubated overnight at 4°C with monoclonal antibodies against tubulin (Millipore, catalog # MAB1864) and acetylated α-Tubulin (Sigma Aldrich ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... cells were collected and washed twice with 1 ml of PBS prior fixation with cold methanol (10 min) or 4% PFA (15 min. Sigma, MO, USA).
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibody 1:12000 mouse anti-α-Tubulin antibody (SIGMA, Cat. # T5168). Followed by 1h incubation with secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with the following primary antibodies at 4°C overnight: rabbit anti-KCC2 (1:1000, Cat#07-432, Millipore, RRID: AB_310611) and goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or μ rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01 ...
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bioRxiv - Immunology 2022Quote: ... and then incubated overnight at 4°C with 1:1000 dilution of primary antibody - either anti-p52/p100 (Millipore Sigma Cat#05361) or anti-β-Actin (Cell Signaling Technology Cat#3700) ...
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bioRxiv - Biophysics 2020Quote: ... 20 µL of 10 % Tween® 20 and 20 µL of a potassium phosphate buffer (4:5 mixture of 1 M monobasic and dibasic potassium phosphate, Sigma Aldrich, USA) were added as well as 10 µL of a 2 nmol thiol-modified single-stranded DNA solution (5’-T20-SH-3’ ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Developmental Biology 2020Quote: ... Nonspecific antigens in the tissue sections were blocked in 4% normal goat serum in PBS-tween and the anti-acetylated α tubulin (1/2000, Sigma # T6793) antibody was incubated with the tissue overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... pre-coated for 1 h (4 degrees) with 50 μg/mL collagen (as prepared below) and 10 μg/mL fibronectin (Sigma-Aldrich, FL1141). For 3D cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from pulverized postmortem brain tissue (0.7g) using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in a phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated 18 hours at 4°C with mouse anti-TH (1/5000, MAB318, Sigma-Aldrich, Saint Louis, MO, USA) in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... ovaries were incubated overnight at 4°C under agitation with the primary antibody (anti-pTyr (4G10) (05-321, Millipore Sigma, 1:40), anti-pERM (3141S ...
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bioRxiv - Physiology 2022Quote: ... It was then finely minced and digested overnight on a rocker at 4 °C in 1 mg/mL protease XIV (Sigma-Aldrich, UK), 0.005% trypsin (Lonza ...