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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The sections were then washed with PBS and blocked with 10% goat serum overnight followed by another overnight incubation at 4°C with monoclonal antibodies against laminin (1:200; Sigma Aldrich, L9393) and collagen IV (1:400 ...
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bioRxiv - Biophysics 2024Quote: ... elution fractions were combined and concentrated to 0.5 – 1 mg/mL using Amicon Ultra-4 30 kDa MWCO centrifugal filter (Cat #: UFC803024, Millipore Sigma, Burlington, MA) TEV protease in 25 mM HEPES buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
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bioRxiv - Physiology 2024Quote: ... muscle sections were incubated overnight at 4°C with a primary antibody against laminin (Millipore-Sigma, Cat. No. L9393, 1:100 dilution) to label the basal lamina surrounding myofibers ...
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bioRxiv - Physiology 2024Quote: ... muscle sections were incubated overnight at 4°C with a primary antibody against laminin (Millipore-Sigma, Cat. No. L9393, 1:100 dilution) to label the basal lamina surrounding myofibers ...
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bioRxiv - Molecular Biology 2024Quote: ... Exponentially proliferating cells were grown to confluence and then left at confluence for 48 hours before incubation in differentiation media (DMEM supplemented with 10% FBS, 4 mM L-Glutamine, 100 U/ml penicillin/streptomycin, 1 μM dexamethasone (Sigma, D-2915), 1 μg/ml insulin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated 18 hours at 4°C with mouse anti-TH (1/5000, MAB318, Sigma-Aldrich, Saint Louis, MO, USA) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were supplemented with high glucose DMEM containing 100 µM 4-HDHA conjugated to 1% fatty acid-free BSA (w/v) (Sigma-Aldrich; A6003). Cells were cultured with 4-HDHA for 24 hours prior to harvest ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were diluted in 10% goat serum and incubated for 18 hours at 4°C with laminin rabbit anti-mouse antibody (1:250, Sigma-Aldrich, L9393). Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were subjected to selection by treatment with 4 μg ml−1 of puromycin (#P8833; Sigma-Aldrich, St Louis, USA). The cells were then single colonized by the limiting dilution cloning method in 96-well plates and expanded ...
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bioRxiv - Immunology 2022Quote: ... Lentivirus diluted in culture media and supplemented with 4 μg/mL protamine sulfate (Sigma Aldrich, stock is 1 mg/mL in PBS) was added to cells ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was cut into two pieces between 55 and 70 kD ladder bands followed by overnight incubation at 4□C with either monoclonal anti-histidine (1:2000) (cat#MAB3834, Millipore Sigma, USA) or anti-GST (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... It was first dissolved in 70% ethanol to yield a stock solution with 200 μg/μL concentration which was then diluted 1:4 with artificial cerebral spinal fluid (ACSF: Sigma-Aldrich, Australia) to a final concentration of 40 μg/μL ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were then added and samples incubated overnight at 4°C in blocking solution at the following dilutions: mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), mouse α-V5 (SV5-Pk1 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cancer Biology 2023Quote: ... were recovered from Matrigel using 3 mM EDTA in DPBS, washed, centrifuged (200g, 5 min, 4°C) and lysed with 1x RIPA buffer supplemented with PPI (1:100, Sigma- Aldrich, 04693132001), and benzonase (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... stratified at 4◦C for two days and plated on ½ MS medium supplemented with 1% sucrose and B5 Vitamins (Sigma; https://www.sigmaaldrich.com). Plates were incubated at 22°C in constant light (80 µmol m-2s-1) ...
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bioRxiv - Cell Biology 2023Quote: ... and were used at the indicated final concentrations in M2 medium: STLC 1 µM or 4 µM (S-Trityl-L-cystein, Sigma-Aldrich, 164739), Nocodazole 0,4 µM (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were incubated overnight at 4°C at the following concentrations in IF Buffer: mouse anti-α-tubulin (DM1α, 1:1000, Sigma T6199), rabbit anti-rat kangaroo-Kid (3μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Cell Biology 2023Quote: ... Dissected larvae were then blocked either for 1 hour at room temperature or overnight at 4°C in 1X PBS containing 0.1% Triton-X and 1% BSA (Sigma-Aldrich, Cat# A7906). Dissections were incubated in primary antibodies overnight at 4°C and secondary antibodies for 2-4 hours at room temperature or overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and then were incubated overnight in 4° in a cocktail of mouse anti-OXT (MAB5296, 1:1000, EMD Millipore Corp, Darmstadt, Germany) and guinea pig anti-AVP (403 004 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubation at 4°C overnight with a mixture of the following primary antibodies: anti-ChAT antibodies (1:50, AB144P; Millipore, UK; RRID:AB_2313845); anti-GFAP antibodies (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... A post-nuclear supernatant was prepared by centrifugation at 2,000 x g at 4°C for 10 min to which 1% NP-40 (EMD Millipore, Billerica, Massachusetts) and 30mM 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPD ...
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bioRxiv - Neuroscience 2024Quote: ... followed by primary antibodies 24-48 hours at 4°C with primary antibodies against BRN3A (1:50 dilution; Millipore Cat# MAB1585, RRID:AB_94166), RBPMS (1:200 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in antibodies for 48h at 4 °C in a blocking solution and immunolabeled with chicken anti-Nefh (1:5000, EMD Millipore, AB5539), mouse anti-Syn1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed brains were quickly rinsed 3 times and then washed 4 x 10 min with phosphate buffered saline (pH 7.4) with 0.5% Triton X-100 (PBST) (Sigma-Aldrich, 9002-93-1). Brains were next blocked with 5% Normal Goat Serum (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected larvae were then blocked either for 1 hour at room temperature or overnight at 4°C in 1X PBS containing 0.1% Triton-X and 1% BSA (Sigma-Aldrich, Cat# A7906). Dissections were incubated in primary antibodies overnight at 4°C and secondary antibodies for 2–4 hours at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked in PBS buffer with 0.1% Tween20 and 5% non-fat dried milk for 1h at room temperature and incubated overnight at 4°C with the following primary antibodies: anti-pan-ADPr (MABE1016, Sigma, 1:1500), which binds both MAR and PAR marks ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in TBS-T and incubated overnight at 4 °C in the same buffer with anti-TH monoclonal antibodies (1:500; Millipore, Stockholm, Sweden). Immunoreactivity was detected using a secondary antibody conjugated with Alexa fluorescent dye (1:400 ...
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bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed cells on coverslips were incubated overnight at 4°C with primary antibodies anti-FLAG (Sigma, Cat No 1804, 1:1000 dilution), and anti-V5 (Sigma ...
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bioRxiv - Genetics 2024Quote: ... were incubated for 1 h at 4°C on a rotating wheel with 15 μl of packed anti-FLAG M2 affinity gel (Sigma-Aldrich, #A2220), which had been washed twice with lysis buffer prior to use ...
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bioRxiv - Cancer Biology 2024Quote: ... Myc (CST 71D10, 1:1000), p-EGFR Y1068 (CST 3777S, 1:1000), EGFR (CST 4267, 1:1000) and β-actin (Sigma A1978, 1:10000). Densitometry analysis was performed with ImageJ software (NIH).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM EDTA, 1% NP40, 0.1% SDS, 0.5% Deoxycholate, 10 mM DTT, 0.5% PVPP, 1% protease Sigma inhibitor and 1 tablet of Roche protease inhibitor for 50 ml of buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Human ITGB1 (encoding integrin β1) primer #1 (H_ITGB1_2) and ITGB3 (encoding integrin β3) primer #1 (H_ITGB3_1) were purchased from Sigma-Aldrich.