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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
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bioRxiv - Bioengineering 2022Quote: ... and 10x DMEM (First Link Ltd., UK) were mixed at a 4:1 volume ratio and neutralized with 5 M NaOH (Sigma, Canada). Fast Green (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa and KMS-12-PE cells by spin-infection (2500 rpm centrifugation at 25 °C for 1 hour) in the presence of 4 µg/mL polybrene (Cat#S2667, Sigma-Aldrich). Stably transduced cells were selected either by antibiotic selection (2 mg/mL hygromycin (Cat# 10687010 ...
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bioRxiv - Genomics 2020Quote: ... the fixed cells were pelleted by centrifugation at 3000 rcf for 10 mins at 4 °C and washed twice with 1X DPBS containing 1% BSA and 0.4U/µl RNase inhibitor (Sigma Aldrich, 3335399001). The cells were then counted and the concentration was adjusted to be close to 1000 cells/µl ...
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bioRxiv - Immunology 2021Quote: ... overnight under gentle agitation at 4°C and eluted with lectin elution buffer containing 1 M Methyl a-D-mannopyranoside (M6882, Millipore Sigma). The resulting solution was dialyzed in PBS and further purified by size exclusion chromatography using Sephacryl S-500 HR resin.
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was pre-cleared with agarose beads (1 h, 4 °C) and incubated with 50 µl of saturated anti-V5 beads (Sigma A7345) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with a goat anti-choline acetyltransferase antibody (ChAT; 1:1000; Millipore Cat# AB144P, RRID:AB_2079751) in Tris-Triton buffer containing 2% donkey serum and 0.2% Triton X100 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by an overnight exposure at 4 °C in the same buffer to the following primary antibodies: (a) rabbit anti-mCherry polyclonal antibody (1:5000, Millipore #AB356482); (b ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were first incubated for 1 h at room temperature in a blocking solution containing 4 % bovine serum albumin (Sigma-Aldrich) in PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for two days at 4°C in solution containing the primary antibody for choline acetyltransferase (Goat-ChAT, 1:100 dilution, Millipore AB144P). When enhancement of GCaMP ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2021Quote: ... USA) were selected and coated by polymerizing a dopamine solution of 4 mg ml-1 dopamine hydrochloride (99%, Sigma-Aldrich, H8502) in 10 mM Tris-HCl buffer at pH 8.5 ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were adjusted to 1-2 mg total protein in 1 mL and incubated overnight at 4°C with 25 µL of packed Monoclonal Anti-HA Agarose (Sigma-Aldrich). Following incubation ...
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bioRxiv - Neuroscience 2021Quote: ... for 1hour at RT and then incubated overnight at 4°C with a mouse anti-βIII-Tubulin (TUJ1, Sigma, 1/500) and a rabbit anti-ISLET1 (Abcam ...
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bioRxiv - Biophysics 2020Quote: ... CD spectra were recorded using a Jasco J-185 spectrometer (ATA Scientific) at 4°C using a 1 mm quartz cuvette (Sigma-Aldrich). Data were recorded using a speed of 20 nm min-1 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 4 °C with the respective species-specific fluorescently-labelled antibodies and DAPI (1 μg/mL, Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated for 24 hours with either 1 mM 3-Hydroxy-1,2-dimethyl-4(1H)-pyridone (Deferiprone/DFP, Sigma-Aldrich, 379409), or incubated in Earl’s balanced salt solution (EBSS ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with the primary antibodies (12h at 4 °C): anti-ChAT (1:500, goat, Sigma-Aldrich, AB144P). Then the sections were washed in PBS five times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with rabbit anti-HA antibody (1:1000, Cat no. H6908, Sigma-Aldrich, USA). For c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the supernatant incubated for 1 hour at 4 °C with continuous agitation with 100 μL of Sepharose beads (Sigma-Aldrich). The extract was then further centrifuged at 500 g for 2 minutes ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Microbiology 2022Quote: ... The membrane pellets were solubilized in 4 mL Wash buffer with 1% Triton 100-X (Sigma-Aldrich, Saint Louis, MO, USA). The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension ...
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bioRxiv - Immunology 2022Quote: ... 50% of the medium was replaced with growth medium additionally supplemented with 4 μM cytosine-1-β-D-arabinofuranoside (Sigma-Aldrich).
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Biochemistry 2022Quote: ... the cells on the reverse face were fixed in 4% paraformaldehyde phosphate buffer solution (Wako) and then stained by 1% Crystal violet (Sigma-Aldrich) and 2% ethanol.
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bioRxiv - Cell Biology 2022Quote: ... Then the sections were incubated overnight at 4°C in 0.1% PBST containing the monoclonal antibody NeuN (Sigma-Aldrich, 1:1500). After three washes in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with the primary antibodies (Abs): 1:400 rabbit anti-GFAP (EMD Millipore catalog#ABS5804); 1:1000 rabbit anti-Iba1 (Wako catalog#019-19741) ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibodies: 1∶15000 rabbit anti-V5 antibody (SIGMA, Cat. # V8137), and 1:12000 mouse anti-α-Tubulin antibody (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...