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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs were cultured on Matrigel-coated coverslips and fixed at day 60 of differentiation in 4% Roti-Histofix (Carl Roth) at RT for 10 min and blocked with 1% Bovine Serum Albumin (BSA; Sigma-Aldrich) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
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bioRxiv - Neuroscience 2023Quote: ... Then the tissue was incubated overnight at 4°C in a mouse monoclonal anti-chondroitin sulfate (C8035; Sigma-Aldrich; 1:500) and a rabbit polyclonal anti-PV (ab11427 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated for 48 hr at 4°C with mouse anti-TH antibody (1:2,000; Millipore, Burlington, MA) in 0.1 M PBS containing 2% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... and the resulting protein was mixed with 5 molar equivalents of pSer-maleimide linkers at a concentration of 1 mg/ml for 16 hours at 4°C in tris-buffered saline (TBS, Sigma Aldrich) at pH 7.2-7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... the neurons were plated in 4-well plates coated with poly-L-lysine (1 mg/ml) (Cat. #: P2636-500MG, Sigma-Aldrich) and homemade laminin (Chauvet et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 mg of minced tissue was digested on ice for 4 minutes in 1 ml of Collagenase/Elastase/Dispase enzyme mix (6 mg/ml Collagenase A (Sigma, 10103578001), 4.3 units/ml Elastase (Worthington ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with A-RPMI and cultured from day 4 to day 8 in A-RPMI supplemented with 1 μM retinoic acid (Sigma R2625) and 100 ng/ml FGF2 ...
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bioRxiv - Neuroscience 2023Quote: ... we blocked membranes with 5% milk in PBS for 1 hour at room temperature and probed overnight with rocking at 4°C with the following primary antibodies: mouse anti-FLAG clone M2 (1/1000 dilution, Sigma # F1804), mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam ...
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bioRxiv - Immunology 2022Quote: Renal leukocytes and splenocytes were stimulated with phorbol myristate acetate (10 ng/ml) and ionomycin (250 ng/ml) for 4 hours with the addition of brefeldin A (1 μg/ml; all Sigma Aldrich) and monensin (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in anti-NeuN primary antibody (1:5000; monoclonal-Mouse Cat# MAB377; Millipore, California USA) in PBSTx with 1% normal goat serum (NGS ...
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bioRxiv - Bioengineering 2022Quote: ... The mouse was then fixed by transcardial perfusion with a 50 mL fixation solution containing 1×PBS and 4% w/v paraformaldehyde (PFA) (Sigma Aldrich). Mouse brain was harvested ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures (30 min, 4°C) with the surface antibody CD34-PE (clone AC136, dilution 1:50, MACS) and DAPI (Sigma-Aldrich), according to manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Mice were euthanized and blood was collected and immediately mixed with 1 ml 4% sodium citrate followed by addition of Histopaque-1077 (catalog no:10771, Sigma-Aldrich). This mix was centrifuged at 400 g for 30 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and left for 4 hours before the media was exchanged with fresh media and containing 1 µM doxorubicin (#44583, Sigma-Aldrich) or 20 uM nutlin3a (444152 ...
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bioRxiv - Genomics 2022Quote: ... and left for 4 hours before the media was exchanged with fresh media and containing 1 µM doxorubicin (#44583, Sigma-Aldrich) or 20 uM nutlin3a (444152 ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then incubated overnight at 4°C in primary antibody solutions with anti-α-tubulin (mouse monoclonal, 1:1,000, T9026, Sigma-Aldrich) or anti-TOMM20 (Rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... promotes esophageal eosinophilic infiltrates and food impactions (28, 35) and oral administration of the carcinogen 4-nitroquinoline 1-oxide (4NQO; N8141, Sigma-Aldrich) induces esophageal tumors that recapitulate histological and molecular features of human ESCC (36 ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich] ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated overnight in a humidity chamber at 4 °C in the primary antibody master mix diluted in TBS with 1% BSA (Sigma Aldrich). Slides were washed twice in 0.1% TWEEN20 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at least 12 larvae were pooled together per sample in 1.5 ml tube and incubated for 1 h at room temperature in 200 µl of the extraction buffer: 4% IGEPAL (Millipore, Sigma) in 100% isopropanol ...
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bioRxiv - Bioengineering 2023Quote: ... 0.1% Tween 20) and incubated for 1 h at room temperature or overnight at 4°C with primary solution (anti-FLAG (Sigma F1804), diluted 1:1000 in 5% milk in TBST) ...
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bioRxiv - Cell Biology 2023Quote: ... were dissected and immunostained as described previously.77 Dissected and fixed tissue was incubated overnight at 4°C with anti-Flag (mouse, 1:200; Sigma F1804) or anti-HA (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C with the primary antibodies (diluted in 1% bovine serum albumin (BSA, cat. no.: A3912-50G, Sigma-Aldrich) in tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred to a 24-well plate and incubated overnight at 4 °C with shaking in 400 µL of primary antibody solution: DAPI 1:1000 (D9542-1g, Sigma-Aldrich), Phalloidin 1:100 (#A12379 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies were then incubated overnight at 4°C in 0.1% BSA in 1x PBS in the following dilutions: 1:1000 RYBP (Millipore Sigma HPA053357), 1:500 Cytokeratin-7 (Millipore Sigma MABT1490) ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL protoplast was mixed with 20 μL DNA (1 – 2 μg total) and 50 μL ice-cold solution 4 (25 g of PEG 6000 or 8000 (Sigma, P2139), 1.47 g of CaCl2.2H2O ...
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bioRxiv - Neuroscience 2024Quote: ... All 4-OHT was freshly prepared on the labeling day in a mix of sunflower seed oil and castor oil (4:1, Sigma-Aldrich) at 10 mg/ml.
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed twice at RT with 0.1% Triton 100X in PBS and then counterstaining with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (1:5000, Sigma Millipore, #MBD0015) in PBS for five minutes ...
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bioRxiv - Biochemistry 2024Quote: ... after which periplasmic expression of nanobodies was induced for another 4 h by addition of 1 mM isopropyl-b-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) to the culture medium ...
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bioRxiv - Microbiology 2024Quote: ... for 1 hr at room temperature or overnight at 4°C followed by incubation in primary antibody (1:3000 mouse-α-FLAG M2, Sigma F3165 ...