Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
-
bioRxiv - Physiology 2020Quote: ... Spermatozoa were activated by mixing 1 µL of diluted sperm (1:4 with modified Leibovitz) with 20 µL of seawater with 30% bovine serum albumin (BSA, Sigma, Spain). One µL of activated sperm was pipetted into an ISAS R2C10 counting chamber (Proiser R+D ...
-
bioRxiv - Immunology 2020Quote: ... Protein pellets were obtained by centrifugation at 12,000 rpm for 15 minutes at 4 °C and subjected to trypsin digestion with sequencing grade trypsin (1:20) (Sigma Aldrich) overnight at 37 °C.
-
bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
-
bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
-
bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2022Quote: ... inhibitors were added a few hours after seeding. Media were replaced three times a week. For actin perturbation we used (Fig. 4) 1 μM Latrunculin A (Sigma-Aldrich); 1 μM Cytochalasin D (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2020Quote: ... in a semi-dry blotting chamber for 45 min at 20 V and 4 °C and crosslinked to the membrane with EDC crosslinking solution (127 mM 1-methylimidazole (Sigma-Aldrich), 163 mM N-(3-dimethylaminopropyl)-N&-ethylcarbodiimide hydrochloride (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
-
bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
-
bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2020Quote: ... RT112M or 639V cell lines were then transduced with the harvested retroviral medium diluted 1:4 with growth medium together with 8 μg/mL of polybrene (Sigma-Aldrich). Cells were then selected for 2 weeks with 200μg/mL of hygromycin.
-
bioRxiv - Bioengineering 2021Quote: ... the cultures were fixed with 4% paraformaldehyde and immunolabeled with anti-neurofilament 200 (NF200) antibodies (1:400, Sigma-Aldrich, catalog # N0142) followed by an Alexa 488 or Alexa 568 conjugated secondary antibody (1:800 ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by incubation at 4°C in a 1:500 dilution of the anti-Calbindin-D-28K antibody (Sigma, CB-955) in blocking solution for 48 hours ...
-
bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
-
bioRxiv - Biochemistry 2021Quote: ... viable cardiomyocytes were plated at a density of 4 × 106 cells/dish on 60 mm Primaria dishes pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). After 18 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were centrifuged for 10 min at 5000Xg and supernatants were diluted 1:1 with PBS (vol/vol) containing protease/phosphatase inhibitors and incubated overnight at +4°C with streptavidin-agarose beads (EMD Millipore). Beads were washed twice with lysis buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue was then washed in PBS containing 0.03% tween-20 for two minutes following overnight incubation at 4°C with anti-versican GAGβ (1:100 dilution, rabbit source, Millipore AB1033). Tissue was then washed three times in PBS containing 0.03% tween-20 for fifteen minutes followed by secondary antibody staining for one hour at room temperature (anti-rabbit Alexafluor 488 ...
-
bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
-
bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
-
bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
-
bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were grown overnight to exponential phase and five serial dilutions (1/4) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were incubated at the relevant temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
-
bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sections were incubated with primary antibodies overnight in a humidified chamber at 4°C with α-actinin (1:1,000, Sigma, A7811). Slides were incubated with secondary antibody (Alexa Fluor Plus 488 ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysates were then immunoprecipitated overnight at 4°C with TrkA antibody (1:100, 06-574, Sigma-Aldrich, St. Louis, MO, USA) or p75NTR antibody (1:100 ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
-
bioRxiv - Neuroscience 2023Quote: ... every 4th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with mouse anti-FLAG monoclonal antibody (1:500; MAB3118, Sigma-Aldrich) or mouse anti-HA monoclonal antibody (1:500 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... The membranes were incubated overnight at 4°C with gentle shaking with rat anti-DAT (1:1000, Millipore Cat# MAB369, RRID:AB_2190413), rabbit anti-TH (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 µL of this mitochondrial suspension were distributed into 4 tubes and were incubated at 30 °C for 30 min with either 1% (w/v) Triton X-100 (Sigma, T8787) or 50 µg/mL proteinase K (Euromedex EU0090-A) ...
-
Small-molecule ketone esters treat brain network abnormalities in an Alzheimer’s disease mouse modelbioRxiv - Neuroscience 2022Quote: ... We validated that a standard curve made using the kit-supplied standard (0-1 mM BHB) provided linear values up to 4 mM by testing 1-10 mM BHB solutions prepared fresh from powder (Sigma 298360). Blood glucose was evaluated on a drop of whole blood using a commercial glucometer (FreeStyle Freedom Lite).
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were pelleted in a swinging bucket centrifuge (1000 x rcf for 15 min at 4°C) and resuspended in reaction buffer (RNase inhibitor (0.2 U/μl) and 1% BSA (Sigma-Aldrich, SRE0036) in PBS) ...
-
bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
-
bioRxiv - Biochemistry 2024Quote: ... The lysate was cleared by centrifugation (158,000 g, 4°C, 1 h) and incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220) for 1 h at 4°C with rotation ...
-
bioRxiv - Biochemistry 2024Quote: The cationic porphyrin derivative 5,10,15,20-tetrakis (1-methyl-4-pyridinio) porphyrin tetra (p-toluenesulfonate) (TMPyP4) was obtained from Sigma-Aldrich (catalog no. 613560). The synthetic acridine analogue BRACO-19 was obtained from Sigma-Aldrich (catalog no ...
-
bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary antibody is diluted in blocking buffer and slides are incubated in primary antibody overnight at 4°C (ChAT, Millipore, AB144P, goat, 1:200; vGlut1, EMD Millipore, AB5905 ...
-
bioRxiv - Neuroscience 2024Quote: ... To block sodium currents and A-type potassium currents, the extracellular solution contained 1 µM TTX (Alomone, IS) and 4mM 4-AP (Sigma, USA), respectively.