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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cell metabolites were dissolved in 0.5 mL of ice-cold 80% methanol solution containing 1 μg/mL 4-chloro-DL-phenylalanine (Sigma, C6506). The samples were then incubated for 30 min in a -80°C freezer ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% v/v of 4 mM HCl containing 0.1% bovine serum albumin (BSA; A3311; Sigma-Aldrich, St. Louis, MO, USA) as the control of BMP4 treatment ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.4) and Tris-buffered saline (TBS, pH 7.4) and blocked in a solution of 1% human serum albumin (Sigma-Aldrich) and 0.1% Triton-X (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and used to produce CD1d tetramers by combining the biotinylated monomers with fluorochrome-conjugated streptavidin at a molar ratio higher than 4:1 and in presence of 0.05% tyloxapol (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst or 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI)) or ExtrAvidinCy3 (Sigma) solution (1:100 in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst) ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 5% (wt/vol) skim milk at 4°C for 12-16 hours and probed with either anti-FLAG (1:3300; Sigma) or anti-ο−70 (1:3300 ...
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bioRxiv - Immunology 2024Quote: ... Cells were pelleted at 300 × g for 5 min at 4°C and then lysed in 500 mL PBS + 0.1% TritonX-100 + 1 mM MgCl2 + protease inhibitors (Sigma Aldrich) for 1 h on ice ...
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bioRxiv - Microbiology 2024Quote: Differentiated THP-1 cells were plated at 4×10⁴ cells per well in an 8-well Millicell EZ slide (Sigma) and then infected with mEmerald-expressing M ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4% PFA in PBS and exposed to 1:1000 anti-FLAG mouse monoclonal antibody (M2, Sigma), followed by Alexa Fluor 647 anti-mouse antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Cultures (1-4 mL) were seeded to an OD600 of 0.1 in minimal media (1x M9 salts [M6030, Sigma-Aldrich, subsidiary of Merck ...
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bioRxiv - Neuroscience 2024Quote: To visualize projection neurons labeled by BDA we incubated free-floating sections for 1 h at 4°C in 0.05M glycine (Sigma-Aldrich) and preblocked for 1 h at 4°C in 5% normal goat serum (NGS ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were separated from the media by centrifugation at 5000 × g for 15 minutes at 4 °C and resuspended in lysis buffer (400 mM NaCl, 25 mM imidazole, 1 U/mL Turbonuclease (Sigma), and 50 mM Tris-HCl pH 8.0) ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid A samples were resuspended in 30 μl of chloroform/methanol mixture (4:1) for thin-layer chromatography (TLC) analysis using Silica Gel 60 F254 TLC plate (Merck Millipore) with the solvent system consisting of chloroform/pyridine/96% formic acid/water (50/50/14.6/4.6) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation at 4 °C with 20 μl of either Anti-FLAG M2 Magnetic Beads (Sigma M8823) for Flag-TTC5 cell lines ...
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bioRxiv - Physiology 2024Quote: ... Slides were incubated O/N at 4°C using the following primary antibodies: Laminin (rabbit at 1:300, Sigma #L9393), MHC Type I A.4.840 (mouse at 1:30 ...
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bioRxiv - Neuroscience 2024Quote: ... at 20 mg/ml in ethanol was added to a 1:4 mixture of castor oil:sunflower seed oil (Sigma 259853 and Sigma S5007) to give a final concentration of 10 mg/ml 4-OHT ...
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bioRxiv - Neuroscience 2024Quote: ... sections were stained overnight at 4°C with guinea pig anti-Vglut2 antibodies (1:700, Cat. #AB2251-I, EMD-Millipore), and mouse anti-PSD95 antibodies (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rehydrated in PBS0.1M and incubated O/N at 4°C with the following primary antibodies: anti-GFAP-Cy3 (1/1000, Mouse; Sigma) or anti-IBA1 (1/500 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’, 6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... IHC was carried out by incubating the sections overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rabbit anti-NG2 (1:200; AB5320, Millipore), mouse anti-CC1 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hours at room temperature and then incubated for 48 hours at 4°C with primary antibodies: polyclonal rabbit anti-tyrosine hydroxylase (TH; 1:1000; #AB152; Millipore); polyclonal sheep anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells on the coverslips were incubated overnight at 4°C with the appropriate primary antibodies (Table S1) in 1% heat-inactivated bovine serum albumin (BSA; Sigma) and 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated with primary antibodies overnight at 4°C (Goat anti-Choline acetyltransferase (ChAT, 1:200 Millipore, AB144), Goat anti-Orexin-A (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Two to 4 microliters of purified plasmid DNA dissolved in PBS (1 μg/μl) containing 0.025% of Fast Green (Sigma-Aldrich) was injected in the lateral ventricles of each embryo using a glass capillary (World Precision Instruments ...
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bioRxiv - Biochemistry 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (PI(4,5)P2) in chloroform: methanol (Millipore MX0488): water (20:9:1 ...
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bioRxiv - Genomics 2024Quote: ... in 270 μl (Table 4) CD buffer (15 mM NaCl, 10 mM Tris-HCl) with and without hexaaminecobalt(III) chloride (1 mM) (Sigma). DNA probes were incubated at 37 °C for 2 hours to allow a potential B-Z transition ...
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bioRxiv - Bioengineering 2024Quote: ... The slices were then incubated overnight at 4°C with rabbit anti-mouse TH primary antibody (1:1000, Millipore, USA), followed by incubation for 20 min with a biotinylated secondary anti-rabbit antibody (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Probing was performed with the following primary antibodies overnight at 4°C: pDNA-PK (pSer2056; SAB4504169, Sigma-Aldrich, 1:300), pATM (pSer1981 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 1 hour at 4°C with 10% (w/v) trichloroacetic acid (TCA) solution (Sigma-Aldrich, T6399). Wells were washed with water and left to dry at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... EV-enriched fractions (1-4) were pooled and concentrated using Amicon 15 Ultra RC 10 kDa MWCO filters (Merck Millipore). Expi293F-palmGRET EV aliquots in Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Cell Biology 2024Quote: ... Incubation with primary antibodies was performed overnight at 4°C at the following concentrations: FLAG (mouse, 1:500; Sigma F1804), SYP-1 (goat ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1x 30 min with 5×SSCT containing 4′,6-diamidino-2-phenylindole (DAPI, 1μg/ml, #62248, ThermoFischer) and/or Phalloidin Atto 565 (1:50, #94072, Sigma Aldrich), and 1x 5min in 5xSCCT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... a coverslip containing DIV4-6 hippocampal neurons was transferred to a fresh petri dish containing 1 ml of growth medium supplemented with 4 mM kynurenic acid (Sigma, K3375 ...
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bioRxiv - Neuroscience 2021Quote: ... using the same immunofluorescence procedures described above (CaMKIIα 1°: 1:3,000, Invitrogen MA1048; GAD67 1°: 1:3,000, EMD Millipore MAB5406). Images of labeling in the dmPFC were acquired at 10X using a Zeiss AxioImager.M2 microscope ...
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bioRxiv - Biochemistry 2020Quote: The S-variant and RBD coupled bead sets were incubated with SARS-CoV-2 patient sera at 3 dilutions (1/100; 1/1000 and 1/10000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) at room temperature in the dark on a horizontal shaker ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with primary antibodies (NMDAR2A-1: 1000, #ab169873, Abcam; NMDAR2B-1:1000, #610417, BD Biosciences; PSD95-1:2000, #76115, Abcam; GluR1-1:1000, #MAB2263, Millipore) at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Ionised calcium-binding adaptor molecule 1 (Iba-1, 1:500, 019-19741, Wako) and Glial fibrillary acidic protein (GFAP, 1:1000, G3893, Sigma). Slides were washed 3×15 min in PBST and incubated for one hour with Alexa Fluor-conjugated secondary antibodies at 1:1000 in 2% normal goat serum at RT ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-centrin 1 (clone 20H5, Millipore; IF, 1:500 or 1:250 (ExM)) ...
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bioRxiv - Plant Biology 2020Quote: ... and calibrated with a 1:1:1 glucose:fructose:galactose (Sigma Chemicals, St. Louis, Missouri, USA) standard curve for sugar and a glucose (Sigma Chemicals ...
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bioRxiv - Developmental Biology 2023Quote: ... mifepristone (1 µL of 1 mM mifepristone dissolved in ethanol, 1 µM, Sigma-Aldrich), vehicle (0.01% ethanol in ddH2O) ...
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bioRxiv - Cell Biology 2024Quote: ... clone 6-11B-1 (1:1,000 IF, 1:10,000 WB, Sigma-Aldrich T6793; RRID:AB_477585); mouse IgG2a anti-GFP ...
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bioRxiv - Molecular Biology 2023Quote: ... The blood was diluted 1:1 or 1:2 with RPMI-1640 (Sigma Aldrich) medium and resuspended well ...
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bioRxiv - Biophysics 2023Quote: ... packaging plasmids at a 1:1:1:2 molar ratio using polyethyleneimine (Sigma-Aldrich). Viral supernatant was collected 48 and 72 h post transfection ...
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bioRxiv - Bioengineering 2024Quote: ... and 1 ul of 1 mg ml-1 PI (Sigma Aldrich, USA, cat. # P4864) was added to cells ...
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bioRxiv - Cell Biology 2020Quote: ... containing DAPI (1 µg ml-1; Sigma-Aldrich). Cells were imaged using a Zeiss Observer.Z1 microscope (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton-X-100 (Sigma, 9002-93-1); 0.5% sodium deoxycholate (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... anti-ZO-1 (1:500, MABT339, Millipore Sigma), anti-Moesin-1 (1:1000 ...