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Citations for Millipore Sigma :
1401 - 1450 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... and blocked (2% bovine serum albumin, Sigma-Aldrich, 0.25% Triton-X, in PBS; 30 min. at RT). Samples were incubated overnight at 4 °C with primary antibodies against lamin-B1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... at room temperature (RT) for 20min and then blocked in 2% Bovine Serum Albumin (BSA, Sigma, SLBT8252) in 1X PBST for at least 60 min at RT ...
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bioRxiv - Pathology 2023Quote: ... with colon fragments being collected and fixed 8 h (RT) in 4% paraformaldehyde (P6148, Sigma Aldrich, USA). Tissue dehydration ...
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bioRxiv - Cancer Biology 2023Quote: ... background signal reduction through incubation for 10 minutes at RT in 0.1 % Sudan Black (Sigma-Aldrich, 199664) took place and sections were washed thoroughly in TBS-T ...
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bioRxiv - Biophysics 2023Quote: ... in PBS for 10 minutes at RT and (2) treated with 0.1 M Hydrochloric acid (Sigma Adrich) for 5 minutes at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... with each wash for one hour at RT and then incubated overnight with Hoechst 33342 (Sigma, B2261) and species-specific secondary antibodies Alexa Fluor 488 ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were blocked at RT for 1 hour with 5 % milk diluted in PBS (Sigma-Aldrich, P4417) containing 0.1% Tween-20 (PBS-T) ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 10 min at RT with 0.1% [w/v] sodium borohydride (NaBH4, Sigma 452882) in PBS to reduce GA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were cloned into pET28a (Novagen), pET28 His6 Sumo TEV LIC (1S ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... PCR was performed using invadolysin primers (Sigma) designed to selectively amplify the invadolysin transgene ...
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bioRxiv - Developmental Biology 2019Quote: ... and PCR Grade Proteinase K (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... 1x PCR buffer (pH=8.7; Sigma, Germany) and 1 μl (30–50 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 8.75 µl of PCR water (Sigma). Reactions were performed on an Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... PCR II products were purified by Millipore MultiScreen® PCRμ96 plate according to manufacture instructions and eluted in 30 μL of nuclease-free water (DEPC) ...
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bioRxiv - Cell Biology 2021Quote: ... PCR primers were purchased from Sigma-Aldrich. Zebrafish primer sequences have been previously published (Lim et al. ...
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bioRxiv - Neuroscience 2019Quote: RedTaq ReadyMix PCR Reaction Mix (Sigma Aldrich), 1 µl of cDNA (obtained as described above ...
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bioRxiv - Immunology 2022Quote: ... The PCR products and pET16b vector (Novagen) were digested with NdeI and XhoI at 37°C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and Expand high-fidelity PCR (Sigma-Aldrich) were used to synthesize and amplify the cDNAs ...
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bioRxiv - Microbiology 2019Quote: ... PCR primers were purchased from Sigma-Aldrich and are listed in S7 Table ...
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bioRxiv - Genomics 2021Quote: ... PCR primers were obtained from Sigma-Aldrich at 100 µM concentration in H2O ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and eluted with 15 μL of nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing ...
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bioRxiv - Physiology 2023Quote: ... REDExtract-N-Amp PCR ReadyMix (R4775, Sigma) was used ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing.
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bioRxiv - Immunology 2023Quote: ... and the following PCR primer sequences (Sigma): Gapdh (F ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR primers were purchased from Sigma, and the Biotin-TEG DNA antisense oligos were designed in Stellaris probe designer and purchased from IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification for each gene sequence was performed with 50 ng of genomic DNA in a 20 µL reaction using a KAPA Taq PCR Kit (Sigma-Aldrich, St Louis, MO, USA) and the following cycle conditions ...
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bioRxiv - Plant Biology 2021Quote: ... 5’ RACE PCR was performed with 2 µl of 1:5 diluted cDNA in a 20 µl reaction with the KAPA2G Robust PCR Kit (KK5501, Sigma-Aldrich, St. Louis, Missouri, USA) buffer B ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The amplified DNA was then purified by depositing the PCR products in a 96-well MultiScreen PCR plate (Millipore). A vacuum of 15 inHg was applied to the wells containing the PCR products until all the wells were dry (12-15 minutes) ...
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bioRxiv - Microbiology 2020Quote: ... Detection was via polyclonal goat secondary antibodies: an ATTO647N-conjugated anti-mouse antibody was purchased from Sigma Aldrich, while an AlexaFluor488 (AF488)-conjugated anti-rabbit antibody was purchased from Invitrogen.
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bioRxiv - Developmental Biology 2021Quote: ... Detection was done with donkey anti-mouse IgG horseradish peroxidase at 1:5000 (1:5000, Millipore Cat#AP308P) and Luminata Crescendo Western HRP substrate (Millipore Cat#WBLUR0100) ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection of Gpd1-TAP (p1291) was carried out by polyclonal rabbit peroxidase α-peroxidase (PAP; Sigma- Aldrich; P1291) at a dilution of 1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins of interest were visualized with the Amersham ECL Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105) or LiteAblot TURBO Extra-Sensitive Chemioluminescent Substrate (EuroClone ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was achieved using appropriate horseradish peroxidase-labeled secondary antibodies and Immobilon Western chemiluminescent HRP substrate (Millipore Sigma). Band intensity was determined with ImageJ 2.0.0-rc-69/1.52p software (http://imagej.nih.gov/ij ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary detection antibody and immunoblots were developed with Forte HRP substrate (Millipore, Dachstein, France). Anti-Actin antibody m25 (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... Detection was performed using horseradish peroxidase conjugated secondary mouse or rabbit antibody and chemiluminescence HRP substrate (Millipore #WBKLS0100).
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... For protein detection: cell lysates were prepared in RIPA lysis buffer containing protease and phosphates inhibitors (Sigma-Aldrich) and Western blotting was performed as described (69) ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed and incubated in HRP-conjugated secondary antibody before detection using Immobilon ECL Ultra substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... All wells were treated with the fluorescent detection probes 2’,7’-dichlorofluorescein diacetate (10µM, DCF-DA; Sigma, D2926) or 4,5-diaminoflurescein diacetate (5µM ...
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bioRxiv - Immunology 2023Quote: ... and plates were probed with IgG1-and IgG2a-specific secondary alkaline phosphatase (AP)-conjugated detection antibodies (Sigma-Aldrich #SAB3701172 and #SAB3701179 ...
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bioRxiv - Plant Biology 2023Quote: ... The enzyme complex used for detection consisted of 0.40 mg mL-1 glucose oxidase (G2133, Sigma-Aldrich, Spain), 0.05 mg mL-1 horseradish peroxidase (77332 ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Immunology 2021Quote: ... and then developed at RT by addition of 100 μL of p-nitrophenyl phosphate (pNPP) substrate (Sigma-Aldrich) per well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma, Cat N° D9663-10ML) in 0.1% PBS-Triton X-100 ...