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Citations for Millipore Sigma :
1201 - 1250 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Nested PCR was performed by two successive PCR reactions using KOD Xtreme polymerase (EMD Millipore #71975) and following set of primers to amplify the genomic region of INSM1-3’UTR:
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bioRxiv - Cell Biology 2020Quote: PCR-based homologous integration cassettes were amplified using the ExpandTM High Fidelity PCR system (Millipore Sigma) from the appropriate plasmid with 80 bp flanking homology to replace the 3’ end of mto2 (primers designed with PPPP at www.bahlerlab.info/resources/)(Bähler et al. ...
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bioRxiv - Microbiology 2023Quote: The DNA was then subjected to conventional PCR using JumpStart REDTaq ReadyMix PCR Reaction Mix (SIGMA) and genus-specific primers for bacteria detection in human lung tissues ...
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bioRxiv - Immunology 2023Quote: The purified libraries were indexed by PCR using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004) and sample-specific i5 primers (10x genomics PN2000095 ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed three times with PBS (with MgCl2 and CaCl2, Sigma D8662), then fixed for 10 min in 3% Methanol-free PFA (Alfa Aesar ...
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bioRxiv - Developmental Biology 2019Quote: ... washed 3 times and incubated with streptavidin Cy3 (Sigma, 1:500) for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... and immunoprecipitated a second time using FLAG M2 antibody (Sigma-Aldrich) and Protein G magnetic beads (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed 3 times with PBS (Merck Millipore, Billerica, Massachusetts, USA) containing 0.32 % (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... retention times and co-injection spiking of authentic standards (Sigma, Carotenature). Quantification was performed based on β carotene calibration curves prepared with an authentic standard (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... and gently washed three times with 0.1% Saponin (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Biophysics 2020Quote: ... Cells were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before blocking with 5 % BSA (Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times with PBS-Tween 20 (1379, Sigma) 0.1% and incubated with phalloidin for 30 min and DAPI (D9542 ...
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bioRxiv - Neuroscience 2022Quote: ... the coated slide was washed 5 times with SSC 2X (Sigma) followed by 2 washes with dH2O ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Pellets were rinsed three times in Milli-Q (mQ) water (Millipore). After the final centrifugation ...
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bioRxiv - Pathology 2022Quote: ... Samples were washed three times and incubated with DAPI (Sigma, B2883) for 1 minute ...
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bioRxiv - Genetics 2019Quote: ... washed in PBS 3 times and mounted with Fluoroshield™ (Sigma). Images were acquired on an Axio Observer microscope (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... After washing three times with PBS containing 0.1% Tween-20 (Sigma) the membranes were developed using ECL reagent (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were treated with 1µM flavopiridol for the indicated times (Sigma). siRNA transfection was performed using the Dharmafect 4 reagent (Dharmacon ...
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bioRxiv - Microbiology 2021Quote: ... The filters were rinsed 3 times in TBS + 0.05 % Tween (Sigma), followed by one final rinse in PBS before being dried overnight at room temperature and scanned on a LI-COR Odyssey Infrared Imager model 9120 ...
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bioRxiv - Genomics 2020Quote: ... Samples were washed 3 times in 30KDa amicon columns (MilliPore # UFC5030BK) and amplified with 10 PCR cycles.
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bioRxiv - Biophysics 2020Quote: ... Cells were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before blocking with 5 % BSA (Roth ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with DMEM (D4947, Sigma-Aldrich), supplemented with GlutaMAX (35050061 ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-FLAG antibodies (Sigma-Aldrich, St. Louis, USA; 5,000 times dilution), anti-MYC antibodies (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2022Quote: ... anti-HA antibodies (Sigma-Aldrich, St. Louis, USA; 2,000 times dilution), or anti-GFP antibodies (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and three times with milliQ before mounting in Mowiol (Sigma, #81381). After overnight hardening of the mounting medium at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 3 times with PBST and SigmaFAST OPD (Sigma) added ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed two times with sterile PBS (P4417, Sigma-Aldrich) before fresh medium was added ...
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bioRxiv - Genetics 2023Quote: ... pellets collected by trypsinization and washed three times with PBS (Sigma). The cell pellet was resuspended in 20 μl lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times in 1x PBS (phosphate buffered saline; EMD Millipore) for 30 min and stored at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed three times with 1M Sodium Acetate solution (Sigma 711096) and three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... One quarter was washed two times with PBS (P4417, Sigma-Aldrich) to remove remaining medium ...
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bioRxiv - Cell Biology 2023Quote: ... Dishes were washed three times following fixation with 1x PBS (Sigma) for 10 minutes.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Slides were washed three times in xylene substitute (Sigma-Aldrich, A5597) for five minutes each wash step ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was stripped a final time using ReBlot Plus (Millipore) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times in 1x PBS (phosphate buffered saline; EMD Millipore) and placed in 30% sucrose for ∼1h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Spheres were dissociated at each time point using Accumax (Sigma Aldrich) and live cells were sorted using the BD FACS Aria II.
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bioRxiv - Molecular Biology 2021Quote: ... and universal PCR primer (Sigma-Aldrich) were used for RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were plate purified (Millipore Multiscreen filter plates ...
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bioRxiv - Microbiology 2021Quote: ... PCR-based test (Sigma, cat# MP0025) was used to validate the absence of potential mycoplasma contamination in all mammalian cell lines ...
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bioRxiv - Immunology 2020Quote: ... 1X PCR buffer containing MgCl2 (Sigma), 250 µM dNTP (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product and pET-30b (+) (Novagen) were digested with XbaI and XhoI restriction enzymes ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR primers (Sigma Aldrich, Taufkirchen, Germany) and probes (Roche Universal Probe Library ...
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bioRxiv - Molecular Biology 2020Quote: ... and enhanced chemiluminescence-based detection of FLAG was performed using Immobilon Western chemiluminescent HRP substrate (Millipore) and an ImageQuant LAS 500 imager (Cytiva Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Microbiology 2020Quote: ... USA) or the fluorescent signal of each protein was measured with a chemiluminescence detection system (Millipore) using Scion Image software (Scion Corp. ...
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bioRxiv - Microbiology 2020Quote: ... Detection occurred by using specific antibodies against β-actin (1:2500 dilution, Sigma-Aldrich, Munich, Germany), ACE2 ...
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bioRxiv - Pathology 2022Quote: ... The signal intensity of targeted protein was detected using ECL western blot detection solution (Millipore, WBKLS0500).
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bioRxiv - Cell Biology 2022Quote: ... detection was performed with horseradish peroxidase (HRP)-conjugated antibodies and Luminata Forte Western HRP Substrate (Millipore) or Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...