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Citations for Millipore Sigma :
1351 - 1400 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... anti-HOIP antibodies used for the detection of human HOIP (Aviva systems biology, ARP43241_P050, and Sigma, SAB2102031), anti-HOIL-1L antibody (Merck Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... The reaction was developed for 1 min using a Luminata Crescendo Millipore immunoblotting detection system (Millipore, WBLUR0500).
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bioRxiv - Cell Biology 2021Quote: ... and revelation was performed using the ECL detection system according to the manufacturer’s instructions (Merck Millipore, WBKLS0500). Scanning for quantification of protein levels was monitored using ImageJ software ...
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bioRxiv - Microbiology 2021Quote: The proximity ligation assays were performed using the Duolink® in situ Detection Reagents (Sigma-Aldrich, DUO92014). For PLA with HIV-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h at room temperature and revealed by an enhanced chemiluminescent detection method (Immubilon Western, Millipore). Protein-loading control was performed with GAPDH or β-actin Ab.
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bioRxiv - Cancer Biology 2021Quote: ... GSL II lectin detection was performed following PNGaseF (250 mU/106 cells, in PBS 1x; Sigma-Aldrich) enzymatic digestion to exclude N-glycan-associated N-acetylglucosamine residues contribution ...
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bioRxiv - Immunology 2022Quote: ... detection of the HRP signal was done with substrate buffer (1 tablet o-Phenylenediamine (Sigma, P-7288) + 35 mL substrate buffer (0.1 M citric acid ...
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bioRxiv - Immunology 2019Quote: ... Detection of primary antibodies was carried out for 1h using the following reagents: fluorescein FITC (Sigma #F2012) conjugated rabbit anti-mouse (1:64 ...
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bioRxiv - Biochemistry 2021Quote: ... Detection of HRP conjugates was carried out with 3,3′,5,5′-tetramethybenzidine (TMB/E, Millipore, Billerica, MA, USA). Detection of AP conjugated antibodies was carried out with SIGMAFAST p-nitrophenyl phosphate tablets (Sigma-N2770).
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bioRxiv - Cell Biology 2021Quote: ... Proximity ligation assay (PLA) was subsequently performed using Duolink® In Situ Detection Reagents Orange (Sigma, DUO92007) according to the manufacturer’s instructions using primary antibodies against PSMB9 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed with peroxidase-conjugated anti-mouse or anti-rabbit IgG (Sigma, St. Louis, MO, USA), and signal was developed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Cell Biology 2022Quote: ... separated by SDS-PAGE followed by western transfer and detection by immunoblotting with anti-Flag (F1804, Sigma) antibody.
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM Tris-HCl (pH7.4)] with ATP detection system containing 5 mM α-ketoglutarate (Sigma-Aldrich 75892), 0.01 mM Ap5A (Sigma D6392) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by detection with HRP-conjugated secondary antibodies and chemiluminescent substrate (Immobilon Western; Millipore-Sigma, Burlington, MA). Bands were visualized with Azure 600 Imaging System (Azure Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... followed by detection with HRP-conjugated secondary antibodies and chemiluminescent substrate (Immobilon Western; Millipore-Sigma, Burlington, MA). Bands were visualized with Azure 600 Imaging System (Azure Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were developed by enhanced chemiluminescence detection system using Immobilon® Forte Western HRP Substrate (Millipore, #WBLUF0500) on Bio-Rad ChemiDoc™ Touch Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Detection of ɣ-Tubulin or pS87 MUS81 was performed using anti-ɣ-Tubulin (1:200, Sigma-Aldrich) or anti-pS87MUS81 (WB 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... PLA DuoLink red or green detection reagents and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunofluorescence detection of CD14 and CD68 was performed using anti-CD14 antibody (Sigma-Aldrich St. Louis, MO) and CD68 antibody (Sigma-Aldrich St ...
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bioRxiv - Biophysics 2024Quote: ... Membranes were then incubated for 3 min with Amersham ECL TM Western Blotting detection reagents (Sigma, GERPN2106) or for 5 min with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... the membrane was subjected to chemiluminescent detection using the Immobilon Western HRP Substrate (Catalog No. WBKLS0500, Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Final detection was carried out using chemiluminescence HRP substrates (Immobilon Western Chemiluminescent HRP Substrate; WPKLS0500; Merck Millipore) and imaged using ChemiDoc XRS+ (Bio-Rad).
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... and permeabilized for 10 min at RT in PBS supplemented with 0.1% Triton X-100 (Sigma Aldrich). Cells were blocked in filtered PBS supplemented with 0.1% Triton X-100 and 5% BSA for 1 h ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... and permeabilized for 10 min at RT in PBS supplemented with 0.1% Triton X-100 (Sigma Aldrich). For blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... reduced with 10mM DTT for 1h at RT followed by alkylation with 40 mM Iodoacetamide (Sigma, U.S.A.) for 1h at RT under dark conditions ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The dechorionated embryos were fixed for 15-20 min at room temperature (RT) with 2% formaldehyde (Sigma) in 5 ml fixing solution (50 mM HEPES-pH-7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... endogenous peroxidase was blocked by incubating for 10 min at RT in 0.3% H2O2 (Sigma-Aldrich #216763). Sections were then incubated in biotinylated goat anti-rabbit antibody (Abcam #ab6720 ...
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bioRxiv - Neuroscience 2020Quote: ... the epidermal sheet was separated from the dermis and incubated at RT in 50% EDTA (Sigma-Aldrich) and 0.05% trypsin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 200-250ng of chromatin were digested for 7 min at RT with decreasing amounts of MNase (Sigma). A 0.5U/µl stock was diluted 1:18 ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were performed in triplicate using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma). Primers used were ...
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bioRxiv - Biochemistry 2020Quote: ... Mixture incubated at RT for 5 minutes and desalted using 30 kDa MWCO spin filters (Millipore-Sigma) into 150 mM ammonium acetate for MS analysis.
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bioRxiv - Biochemistry 2020Quote: ... Mixture incubated at RT for 5 minutes and desalted using 30 kDa MWCO spin filters (Millipore-Sigma) into 150 mM ammonium acetate for MS analysis.
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bioRxiv - Pathology 2021Quote: ... Cryosections were blocked for 90 min at RT with TBS containing 0.2% Triton X-100 (Sigma-Aldrich) and 10% bovine serum (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were blocked for 2h at RT (3% normal donkey serum [Sigma D9663], 0.1% triton-x), stained with 1’ ab for 12h at 4°C (EMD Millipore [ab144P ...
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bioRxiv - Biochemistry 2022Quote: ... RT overnight) before derivatising with 20 µl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane (Sigma, 33148) for ≥1 h ...
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bioRxiv - Neuroscience 2021Quote: ... were fixed for 15 min at room temperature (RT) in 4% (w/v) paraformaldehyde (PFA, Sigma-Aldrich) in distilled phosphate buffered saline (dPBS ...
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bioRxiv - Biochemistry 2021Quote: ... LtaA mutants were incubated for 3h at RT in the presence of 0.5 mM mPEG5K-Maleimide (Sigma) and 0.5% SDS ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from frozen gastrocnemius muscle powder using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... baltica strain #1432 was grown at room temperature (RT) on agar plates (12 g sea salt (Sigma), 1 g yeast extract (Helicon) ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked for 1 hour at room temperature (RT) in PBS containing 2% bovine serum albumin (Sigma-Aldrich). Fixed cells were then incubated with the primary antibody in 2% BSA/PBS solution overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were blocked for 2 hours at RT with blocking solution (PBS with 1% chicken ovalbumin; Sigma). For ELISA inhibition assay ...
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bioRxiv - Immunology 2020Quote: ... for 1 hr at RT and then enzymatically visualized by 3,3’-diaminobenzidine (DAB) enzyme reaction (Sigma-Aldrich). Sections were counterstained with hematoxylin and eosin and images were captured using Zeiss ZEN software on a Zeiss Cell Observer inverted microscope with an Axiocam dual B/W and color camera with a 20x objective ...
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bioRxiv - Neuroscience 2021Quote: ... Japan) were permeabilized at RT for 40 min in PBS containing 0.5% Triton X-100 (Sigma-Aldrich). Sections were then blocked overnight at 4°C in PBS containing 10% normal horse serum (NHS) ...
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bioRxiv - Biochemistry 2020Quote: ... incubated for 2 hours at RT with primary monoclonal mouse anti-FLAG M2 antibody (F1804, Sigma Aldrich) to track migration of 3 x FLAG-tagged eIF2B complex ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 100 mg of tissue or 106 cells were homogenized in 1 mL RNAzol RT (Sigma #R4533) and mRNA was extracted according to the product technical bulletin ...