Labshake search
Citations for Millipore Sigma :
1651 - 1700 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Sections (3µm thick) were then incubated for 40 minutes at RT with 1:1000 dilution of anti-APE1 (HPA002564; Sigma). Detection of primary antibody was performed using the Super-sensitive–Polymer HRP system (Biogenex ...
-
bioRxiv - Immunology 2021Quote: ... Qβ(1668)-VLPs were then derivatized for 30min at RT using dibenzocyclooctyne NHS ester (DBCO cross-linker) (Sigma-Aldrich) in a 5-fold molar excess ...
-
bioRxiv - Immunology 2021Quote: ... Staining was performed for 1 hour at RT and slides were mounted with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich). Confocal images were acquired on an LSM 700 system (Carl Zeiss ...
-
bioRxiv - Microbiology 2020Quote: ... COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at room temperature (RT) followed by permeabilization with 0.2% Triton X-100 (Sigma) for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from approximately 100mg flash-frozen liver tissue following homogenization in RNAzol RT reagent (Sigma-Aldrich). cDNA was synthesized from 1ug RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated for 30 minutes at RT in a homemade Click reaction mixture containing 4 mM CuSO4·5H2O (Sigma), 4 μM Alexa Fluor-647-conjugated azide (ThermoFisher) ...
-
bioRxiv - Physiology 2022Quote: ... Cells were subsequently washed with PBS and incubated with 1.5 mg/ml glycine (Sigma-Aldrich, 10 min at RT) to quench unreacted paraformaldehyde followed by washing with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... the zebrafish larvae were permeabilized for 1h at RT with a blocking buffer [5% BSA (A9647-10G, Sigma-Aldrich), 5% sheep serum (013-000-1210 ...
-
bioRxiv - Cell Biology 2023Quote: ... specimens were prepared in PBS and subsequently fixed for 4 h at RT in fixative (2% glutaraldehyde (Sigma, Germany)/ 4% paraformaldehyde (Merck ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the following changes: 90 minute primary antibody incubation at RT (rabbit α-GST, Sigma Aldrich #G7781, 1:4000) and 30 minute secondary incubation at RT (goat α-rabbit HRP ...
-
bioRxiv - Molecular Biology 2023Quote: ... The signal was developed by incubation with DAB (3,3′-diaminobenzidine) for 10 minutes at RT and counterstained with hematoxylin (Sigma), followed by a wash in 0.02% ammonia water before mounting ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were initially blocked for 2 hours at room temperature (RT) with PBS-T 0.05% containing 10% of a donkey or goat Serum (Sigma). Primary antibodies ...
-
bioRxiv - Immunology 2023Quote: ... and fixed for 3 hr at room temperature (RT) in freshly prepared Carnoy’s fixative [60% anhydrous methanol (Sigma-Aldrich), 30% chloroform (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated for 1 h at RT with secondary AP-conjugated anti-mouse or anti-rabbit antibodies (1:20,000, Sigma), washed three times for 5 min with PBT and once for 15 min in 20 mM Tris-HCl (pH 9.8 ...
-
bioRxiv - Immunology 2023Quote: ... for 20 min in the dark RT and washed with 100 mM ammonium bicarbonate (NH4HCO3) (09830-500G, Sigma-Aldrich) and dehydrated as above ...
-
bioRxiv - Cell Biology 2023Quote: ... BMDMs were crosslinked with 0.4% paraformaldehyde for 10 minutes at room temperature (RT) and immediately quenched with 0.125 M glycine (Millipore Sigma) for 5 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... were added and incubated for 2 h at RT on shaker followed by 3x washes in 0.1% TritonX100 (Sigma) in PBS (Gibco).
-
Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (15,000 rpm, 10 min, RT) and the supernatants were transferred into glass vials containing MTBSTFA (70 μl, Sigma) for derivatization at 70 °C for 1 h ...
-
bioRxiv - Genetics 2023Quote: ... The seminiferous tubules were incubated for 60 min at RT in fixation buffer (100 mM HEPES pH7.4, 4 mM CaCl2, 2.5% glutaraldehyde, 2% PFA, all from Sigma Aldrich) and then the buffer was exchanged with fresh fixation buffer and the samples left overnight fixed at 4 °C ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... PFA was subsequently quenched with 500 mM NH4Cl for 10 min at RT and then cells were permeabilized with 0.1% Triton-X-100 (Sigma) for 10 mins at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were blocked with 5% BSA in PBS for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in 0.5% BSA/1% Tween-20 (Sigma) in PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 10 min at room temperature (RT) and permeabilized for 5 min in 0.5% Triton X-100 (#T9284, Sigma) with 2 mM VRC diluted in PBS on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were incubated and inverted for 10 min at RT with ammonium chloride lyse [NH4Cl 155 mM (Sigma Aldrich), NaHCO3 10 mM (Sigma Aldrich) ...
-
bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
-
bioRxiv - Neuroscience 2024Quote: ... 4%) for 30 min at RT followed by a permeabilization step by using Triton X-100 (0.1%; Sigma-Aldrich) for 15 min at RT or Tween-20 (0.1% ...
-
bioRxiv - Developmental Biology 2021Quote: ... samples were rinsed several times in PBT and treated with imidazole 1M (Sigma, I5513)/PBT supplemented with H2O2 ...
-
bioRxiv - Microbiology 2022Quote: ... FAMEs were identified by comparison of their retention times with those of standards (Sigma) and by their mass fragmentation spectra ...
-
bioRxiv - Microbiology 2022Quote: ... Cysts were generated for indicated times and treated with established antiparasitics (pyrimethamine, sulfadiazine (Sigma)) and 1-hydroxy-2-dodecyl-4(1)-quinolon (HDQ ...
-
bioRxiv - Physiology 2021Quote: ... rAAVs were washed 3 times with PBS using Amicon Ultra Centrifugal Filter Units (Millipore) and resuspended in PBS + 0.001% Pluronic F68 ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were washed 3 times in PBS and fixed in 4% paraformaldehyde (Sigma Alrich) PBS for 20 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Slides with paraffin sections were washed three times in clean xylene substitute (Sigma A5597), then once each in 100% ethanol ...
-
bioRxiv - Molecular Biology 2019Quote: ... Hepatocytes were washed 4 times with ice-cold low glucose DMEM (D6046; Sigma-Aldrich) supplemented with 10 mM Hepes (#15630080 ...
-
bioRxiv - Plant Biology 2019Quote: ... Membranes were washed 5 times and revealed with Immobilon western chemiluminescent HRP substrate (Millipore).
-
bioRxiv - Cell Biology 2019Quote: ... washed 3 times in PBS before incubation in PBS supplemented with 3% BSA (Sigma) for 30 min to block non-specific antibody binding ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were washed three times for 5 min with 0.002 % NP40 (Sigma-Aldrich, i8896) in PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... They were rinsed three times with Hank’s Balanced Salt Solution (HBSS, Millipore Sigma, 55021C), then cut into 1mm3 pieces with surgical scissors ...
-
bioRxiv - Bioengineering 2021Quote: ... They were rinsed three times with Hank’s Balanced Salt Solution (HBSS, Millipore Sigma, 55021C), cut into 1mm3 pieces with surgical scissors ...
-
bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted ten times with IP-Lysis buffer supplemented with 15mM NEM (Sigma) and protease inhibitor cocktail ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were washed 2 times with PBS and fixed in 3.7% formaldehyde (Sigma Aldrich) at RT for 15 min ...
-
bioRxiv - Microbiology 2020Quote: ... washed with PBS three times and permeabilized with 0.2% Triton X-100 (Sigma, T9284)/PBS for 20 min at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Wells were then washed three times with PBS containing 0.05% Tween-20 (Sigma-Aldrich), blocked by incubation overnight at 4°C with 200 μl of 3% nonfat milk-PBS/well and washed again the next morning ...
-
bioRxiv - Microbiology 2020Quote: ... Wells were then washed three times with PBS containing 0.05% Tween-20 (Sigma-Aldrich). One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG ...
-
bioRxiv - Systems Biology 2021Quote: ... cells were washed three times with PBS supplemented with 0.1% Tween-20 (Sigma-Aldrich) (PBS-T ...
-
bioRxiv - Plant Biology 2020Quote: ... and retention times were compared with authentic standards (Sigma Aldrich, St Louis, MO, USA) for volatile identification68.
-
bioRxiv - Immunology 2020Quote: ... washed three times with PBS and permeabilized with 2% Triton X-100 (Sigma-Aldrich) 5% Fetal Bovine Serum (FBS ...
-
bioRxiv - Plant Biology 2021Quote: ... rinsed 2 times with PBS and blocked with 4% Bovine Serum Albumin (BSA, Sigma) for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma) and 10μg/ml DNAse (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: ... Fully differentiated keratinocytes can be passed 3-5 times on Collagen I (Sigma-Aldrich)-coated tissue culture dishes ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with PBS-T and one-step TMB solution (Sigma) was added to the plates ...
-
bioRxiv - Neuroscience 2020Quote: ... They were then washed four times and stained using diaminobenzidine (DAB, Sigma-Aldrich, USA). Subsequently ...