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Citations for Millipore Sigma :
1301 - 1350 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and three times with milliQ before mounting in Mowiol (Sigma, #81381). After overnight hardening of the mounting medium at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 3 times with PBST and SigmaFAST OPD (Sigma) added ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed two times with sterile PBS (P4417, Sigma-Aldrich) before fresh medium was added ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times in 1x PBS (phosphate buffered saline; EMD Millipore) for 30 min and stored at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed three times with 1M Sodium Acetate solution (Sigma 711096) and three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... One quarter was washed two times with PBS (P4417, Sigma-Aldrich) to remove remaining medium ...
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bioRxiv - Cell Biology 2023Quote: ... Dishes were washed three times following fixation with 1x PBS (Sigma) for 10 minutes.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Slides were washed three times in xylene substitute (Sigma-Aldrich, A5597) for five minutes each wash step ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was stripped a final time using ReBlot Plus (Millipore) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times in 1x PBS (phosphate buffered saline; EMD Millipore) and placed in 30% sucrose for ∼1h ...
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bioRxiv - Biophysics 2024Quote: ... After washing the chambers three times with PBS (Sigma-Aldrich, D8537), the surfaces were incubated with 0.5 g/L BSA-Biotin (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... and universal PCR primer (Sigma-Aldrich) were used for RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were plate purified (Millipore Multiscreen filter plates ...
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bioRxiv - Microbiology 2021Quote: ... PCR-based test (Sigma, cat# MP0025) was used to validate the absence of potential mycoplasma contamination in all mammalian cell lines ...
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bioRxiv - Immunology 2020Quote: ... 1X PCR buffer containing MgCl2 (Sigma), 250 µM dNTP (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product and pET-30b (+) (Novagen) were digested with XbaI and XhoI restriction enzymes ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR primers (Sigma Aldrich, Taufkirchen, Germany) and probes (Roche Universal Probe Library ...
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bioRxiv - Molecular Biology 2020Quote: ... and enhanced chemiluminescence-based detection of FLAG was performed using Immobilon Western chemiluminescent HRP substrate (Millipore) and an ImageQuant LAS 500 imager (Cytiva Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Microbiology 2020Quote: ... USA) or the fluorescent signal of each protein was measured with a chemiluminescence detection system (Millipore) using Scion Image software (Scion Corp. ...
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bioRxiv - Microbiology 2020Quote: ... Detection occurred by using specific antibodies against β-actin (1:2500 dilution, Sigma-Aldrich, Munich, Germany), ACE2 ...
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bioRxiv - Pathology 2022Quote: ... The signal intensity of targeted protein was detected using ECL western blot detection solution (Millipore, WBKLS0500).
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bioRxiv - Cell Biology 2022Quote: ... detection was performed with horseradish peroxidase (HRP)-conjugated antibodies and Luminata Forte Western HRP Substrate (Millipore) or Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were taken through a standard immunoblot protocol followed by enhanced chemiluminescent detection (Crescendo ECL, Millipore) using a Lumimager (Fuji ...
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bioRxiv - Microbiology 2019Quote: ... and detection (absorbance at 520 nm) using 0.15% m-hydroxydiphenyl (Sigma-Aldrich, St. Louis, MO, USA) in 0.5% NaOH (Amresco ...
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bioRxiv - Biochemistry 2021Quote: ... Detection of AP conjugated antibodies was carried out with SIGMAFAST p-nitrophenyl phosphate tablets (Sigma-N2770).
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bioRxiv - Molecular Biology 2024Quote: ... PLA DuoLink red or green detection reagents and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... both in blocking solution and detection using the Supersensitive TMB substrate (3,3’,5,5’-tetramethylbenzidine, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies and detection reagents used: tyrosine hydroxylase (anti-rabbit, Abcam, ab137869; anti-mouse, Millipore, MAB318), dopamine transporter (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Blocking and staining were performed with Duolink in situ PLA probes and detection reagents (Sigma-Aldrich). Images were taken by using the Z-stacking function at 40X on a Keyence BZ-X710 microscope and analyzed with the BZ-X analyzer ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used for detection were mouse monoclonal anti-μ-calpain (Sigma-Aldrich, United States) diluted 1:1000 ...
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bioRxiv - Physiology 2023Quote: ... After three washes in a PBS 0.3% tween solution and incubation with the detection reagent (Millipore), the signal acquisition was performed using the Biorad ChemiDoc apparatus.
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bioRxiv - Developmental Biology 2023Quote: ... but those for detection of phosphorylated proteins were blocked overnight in 5% gel fish (Sigma, #G7765). Blots were probed with antibodies listed in Suppl ...
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bioRxiv - Immunology 2023Quote: ... The proximity of the interested molecules was visualized using Duolink In Situ Detection reagents (Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The DuoLink in Situ Detection reagents Orange (λex = 554 nm and λem = 576 nm; Millipore Sigma) was used to determine whether SMAD3 interacts with SP1 in THP-1 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... using rabbit PLA plus and mouse PLA minus probes and red Duolink detection reagents (Sigma-Aldrich). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Wako Lab Chemicals) for 4 hours at RT before dilution with ammonium bicarbonate (20 mM; Sigma) and digestion with trypsin (1/50 (w/w) ...
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bioRxiv - Genetics 2019Quote: ... LK03) were pooled in equimolar ratio and fragmented at room temperature (RT) using DNAse I (Sigma). At different incubation time points aliquots were analyzed on an 1.5% agarose gel while the reaction was temporarily stopped by incubation in a dry ice / ethanol bath ...
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bioRxiv - Neuroscience 2020Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Microbiology 2020Quote: ... left 5 min at RT for cell attachment and fixed with 4% paraformaldehyde (PFA) (Sigma P6148) for 10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-qPCR with the FastStart Universal SYBR Green Master (Sigma-Aldrich, Cat No. 4913850001). Primers are listed in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Biophysics 2020Quote: ... and then incubated for 10 min at RT with 25U benzonase nuclease (Millipore Sigma 70746-10KUN)/50 μL sample ...
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bioRxiv - Molecular Biology 2022Quote: RPA and RT-RPA reactions are performed on a variety of commercially available porous membranes (Millipore GF041 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... for 4 minutes at RT and sections were counterstained with haematoxylin (Sigma Chemie GmbH, Steinheim, Germany), dehydrated in ethanol and mounted with Pertex (Histolab ...
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bioRxiv - Cell Biology 2022Quote: Larval preparations were fixed for 20 min at room temperature (RT) with 4% formaldehyde (Sigma #47608) in 1x phosphate buffered saline (PBS ...