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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Physiology 2024Quote: ... Triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Cat#: F6428 & T2449, Sigma, Free-Glycerol reagents and Triglyceride ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.4) at a concentration of 2.66 mg/mL supplemented with a Roche protease inhibitor tablet and 1% digitonin (Sigma Aldrich). After 2 minutes ...
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bioRxiv - Immunology 2024Quote: Cells were lyzed in RIPA buffer supplemented with protease inhibitors (AEBSF 4 mM, Pepstatine A 1 µM and Leupeptin 0.4 mM; Sigma Aldrich) and HIF-hydroxylase inhibitor (DMOG 1 mM ...
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bioRxiv - Neuroscience 2024Quote: ... sections were subjected to a blocking step with 5% NDS and then incubated overnight at 4 °C with goat anti-Chat (at a dilution of 1:250; #AB144P, Sigma) and Streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cultures were transduced with concentrated lentivirus at an MOI of 10 for 48 hours in 4 µg mL−1 polybrene (Sigma), then selected in normal growth media supplemented with appropriate antibody selection ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were incubated overnight at 4°C in the presence of primary antibodies in PBS-T: rabbit PYK2 antibody (1/500, Sigma #07M4755 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917, 1:500 dilution; anti-GluA1, Merck-Millipore, AB1502 ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Bioengineering 2024Quote: ... Embryos were finally covered with a mixture of hydrocarbon oil (700 and 27 at a 4:1 ratio; Sigma Aldrich) and subjected to microinjection in less than 1h post fertilization ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated at 4 °C overnight with a 1:100 diluted mouse monoclonal anti-type I collagen antibody (C2456; Sigma). For Col-II staining ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... After an overnight incubation at 4 °C with a 1:10,000 dilution of Streptavidin-HRP (Sigma-Aldrich, Cat. No. S5512) in TBS-T plus 3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... was thawed at 4°C and supplemented with 10 U mL−1 horseradish peroxidase (type VI lyophilized powder, Sigma-Aldrich) and 0.4 μL mL−1 hydrogen peroxide solution (30% w/w ...
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bioRxiv - Immunology 2023Quote: ... half of the medium was taken up and Th17 cells were transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... blots were incubated overnight at 4°C at the following dilutions: Mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), Mouse α-V5 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at least 12 larvae were pooled together per sample in 1.5 ml tube and incubated for 1 h at room temperature in 200 µl of the extraction buffer: 4% IGEPAL (Millipore, Sigma) in 100% isopropanol ...
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bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600, 1:500 dilution; GFAP, mouse Sigma G3893 ...
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bioRxiv - Cell Biology 2023Quote: ... Incubation with primary antibodies was performed overnight at 4°C at the following concentrations: FLAG (mouse, 1:1000; Sigma F1804), HA (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were inoculated at MOI 15 in 1 mg/mL of fluorescein isothiocyanate–dextran 4 kDa (FITC-dextran, Sigma-Aldrich) dissolved in CnT-Prime-3D ...
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bioRxiv - Developmental Biology 2023Quote: The testes from 8-week-old C57BL/6J wild-type mice or 4-month-old Foxc2f/+ and Foxc2f/-;Ddx4Cre/+ mice (n=4) were minced and digested in the collagenase type IV (1 mg/mL, Sigma) and DNase I (500μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: 1×105 U2OS IRE1-HaloTag cells were grown on 24 h in Millicell EZ Slide 4-well chamber slides (Millipore), fixed in 4% PFA and permeabilized with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... Colloidal probe cantilevers were immersed for 60 minutes in Tris-buffered saline (TBS; Tris, 50 mM; NaCl, 150 mM; pH 8.5) containing 4 mg mL-1 of dopamine hydrochloride (Sigma-Aldrich), rinsed in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were prepared by an established protocol in Paul et al.[4] and stained using TUB 2.1 monoclonal antibody (1:1000, T4026, Sigma-Aldrich) detected with anti-mouse secondary antibody directly conjugated to Alexa Fluor 568 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Genetics 2023Quote: ... both caudal epididymides were removed and placed in 1 mL of prewarmed DMEM containing 4% bovine serum albumin (Sigma-Aldrich). Sperm were extruded from each epididymis using tweezers and subsequently incubated at 37°C/5% CO2 for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with a 1:200 dilution of the following antibodies overnight at 4°C: anti-trimethyl-Histone H3 (Lys27) (07-449, Millipore), anti-trimethyl-Histone H3 (Lys9 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were separated from the media by centrifugation at 5000 × g for 15 minutes at 4 °C and resuspended in lysis buffer (400 mM NaCl, 25 mM imidazole, 1 U/mL Turbonuclease (Sigma), and 50 mM Tris-HCl pH 8.0) ...
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bioRxiv - Neuroscience 2024Quote: To visualize projection neurons labeled by BDA we incubated free-floating sections for 1 h at 4°C in 0.05M glycine (Sigma-Aldrich) and preblocked for 1 h at 4°C in 5% normal goat serum (NGS ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid A samples were resuspended in 30 μl of chloroform/methanol mixture (4:1) for thin-layer chromatography (TLC) analysis using Silica Gel 60 F254 TLC plate (Merck Millipore) with the solvent system consisting of chloroform/pyridine/96% formic acid/water (50/50/14.6/4.6) ...