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Citations for Millipore Sigma :
851 - 900 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 200nM of gene specific primer- HPLC purified (Sigma-Aldrich, St. Louis, MO) in 25 μL reaction volume ...
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bioRxiv - Developmental Biology 2022Quote: ... on 384-well plates containing 384 primers and Mineral oil (Sigma Aldrich). After sorting ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotide used as primers for amplification (obtained from Sigma-Aldrich or Eurofins) are listed in Supplementary Table S2 ...
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bioRxiv - Biochemistry 2020Quote: ... Powdered chemical reagents and custom DNA primers were purchased from Sigma-Aldrich. Mass spectrometry (MS ...
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bioRxiv - Molecular Biology 2020Quote: ... T7 promoter and T7 terminator primers were obtained from Novagen (Madison, WI). pcDNA3.1 was obtained from Addgene (Cambridge ...
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bioRxiv - Biochemistry 2021Quote: ... All primers used for cloning were designed and ordered from Sigma-Aldrich and are outlined in Table 2 ...
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bioRxiv - Microbiology 2020Quote: ... Oligonucleotide primers were synthesized by Sigma-Aldrich (see Table S1 for sequences). Molecular biology procedures were carried out according to the manufactures’ instructions (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... and primers designed to carry NheI and KpnI restriction sites (Sigma Aldrich). PCR amplified inserts were added at the N-terminus of N protein in pcDNA3.1+ plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... Oligonucleotide primers incorporating desired mutations were synthesised by Sigma Aldrich (Sydney, Australia) and used to generate point mutations in the GlyT2 cDNA/pOTV construct utilising the Q5 site-directed mutagenesis kit (New England Biolabs (Genesearch) ...
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bioRxiv - Plant Biology 2022Quote: ... Primers were designed using Primer3 0.4.0 software and synthesized by Sigma-Aldrich. The presence of pNDN-OGG or pNDH-OCT in F ...
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bioRxiv - Developmental Biology 2022Quote: ... 1ul (10uM [final]) of each of the forward and reverse primers (SIGMA), 2μl of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... All SYBR primers were designed by Sigma Aldrich (see Suppl. Table 1), and Taqman primers were designed by ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1 µl of 10 mM 5′ HEX labelled oligonucleotide primer (Sigma-Aldrich) and 2 µl ddH2O was incubated at 85°C for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Desalted oligonucleotide primers were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) and can be found in following table ...
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bioRxiv - Molecular Biology 2024Quote: ... using a combination of oligo(dT)23 and random hexamer primers (Sigma). The resulting cDNA was amplified in an AriaMX cycler (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... a SYBR-green qPCR reaction was performed with gene-specific primers (Sigma) as per Nolbrant et al ...
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bioRxiv - Cell Biology 2020Quote: ... For genotyping PCR JumpStart RedTaq PCR master mix (Sigma-Aldrich) was used following the manufacturer’s protocol for cycling with an annealing temperature of 55°C and 35 cycles.
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium Deoxycholate) with 5.5μl Protease Inhibitor Cocktail Set III EDTA Free (EMD Millipore Corp.539134-1ML) and 11μl Murine RNase Inhibitor (New England BioLabs Inc® M0314L ...
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bioRxiv - Neuroscience 2020Quote: ... a second set of sections were stained using mouse anti-NeuN (Millipore Bioscience Research Reagents; 1:50,000). Following incubation in primary antibody solution as described above ...
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bioRxiv - Plant Biology 2020Quote: ... DNA digestion was performed using the On-Column DNase I Digestion Set (Cat No.: DNASE70-1SET, Sigma) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... volumes of 3 ml of a set of 48 monomolecular odorants (Sigma-Aldrich, St. Louis MO, USA) were pipetted freshly for each experimental day into 15 ml glass vials (27160-U ...
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bioRxiv - Immunology 2022Quote: ... Kmt2d-SET-fl/fl mice were sensitized with 100 μg ovalbumin (OVA #A5503; Sigma, St. Louis, USA) and 1 μg cholera toxin (CT #101B ...
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bioRxiv - Biophysics 2021Quote: ... bisected ovaries were arranged so that the probed surface faced downwards and set in 2% agar (Sigma). Samples were processed through graded alcohol (70% 1h) ...
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bioRxiv - Genomics 2022Quote: These are a subset of a larger set of hydrophobic polytetrafluoroethylene (PTFE, EMD Millipore, Danvers, MA, USA) fluorophore membrane filters (3 μm pore size ...
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bioRxiv - Neuroscience 2022Quote: ... each barcoded set was blocked in CSM containing 10% (v/v) normal donkey serum (Millipore, S30-100ML) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... DAB (Sigma Fast 3,3 Di-aminobenzidine Tablet Set, D-4418 (Sigma-Aldrich Corp, St. Louis, MO, USA)) was used as a counterstain as previously described (Harris et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% Sodium Deoxycholate) with 5.5µl Protease Inhibitor Cocktail Set III EDTA Free (EMD Millipore Corp.539134-1ML) and 11µl Murine RNase Inhibitor (New England BioLabs Inc® M0314L ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase digestion was performed on-column using the On-Column DNase I Digestion Set (Sigma Aldrich, DNASE70) as instructed ...
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bioRxiv - Genetics 2024Quote: ... The second set of 15ml conical tubes contained yeast cells with 1% acetaldehyde (Sigma, catalog number 402788) diluted in water ...
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bioRxiv - Genetics 2024Quote: ... followed by treatment with RNase-free DNase using the On-Column DNase I Digestion Set (Sigma-Aldrich). RNA integrity and purity were assessed by agarose gel electrophoresis and a Bioanalyzer 2100 ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated for 10 min at RT in DPBS/DNases (Sigma, DN25) solution ...
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bioRxiv - Pathology 2022Quote: ... rinsed in PBS and fixed (4% paraformaldehyde, Sigma, in PBS, 15min., RT), permeabilized (0.1% Triton X-100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were fixed with formaldehyde (Sigma-Aldrich®, 1%, 10 min, RT) and quenched with glycine (0.125 M ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quenching 5 min at RT with 125 mM glycine (Sigma). Cultures were spun down ...
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bioRxiv - Developmental Biology 2021Quote: ... and stored at RT in PBS containing 0.2% gelatin (Sigma Aldrich, #G1393), 0.5% Triton X-100 (Sigma Aldrich ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and coated for 1hr RT with 25 μg/ml laminin (Sigma, L2020) diluted in a buffer containing 150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... permeabilized (1% Triton-X, Sigma-Aldrich, in PBS; 10 min. at RT) and blocked (2% bovine serum albumin ...
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bioRxiv - Immunology 2022Quote: ... fixed for 10 min at RT with 4% paraformaldehyde (PFA: Sigma-Aldrich) in 0.1 M Phosphate Buffer (pH 7.4 ...
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bioRxiv - Genomics 2023Quote: ... RT-qPCR was performed using FastStart Universal SYBR Green Master (Sigma-Aldrich) and primers are given in Supplementary Table 9.
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bioRxiv - Microbiology 2021Quote: ... the mCherry gene was amplified together with the gap1 promoter using primers (Sigma) mentioned in supplementary table 1 and the pRed plasmid (Zacchetti et al ...
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bioRxiv - Microbiology 2019Quote: ... Seven ng of pGCC4fHbpL91543 and 0.5 µM of each pair of primers (Sigma) were used for amplification and incorporation of the desired mutation ...
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bioRxiv - Microbiology 2020Quote: ... All primers are listed in Table 3 and were obtained from Sigma-Aldrich. PCR reactions were visualized and analyzed by gel electrophoresis on 1 % (w/v ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: All chemicals and primers were purchased from Sigma-Aldrich (St. Louis, MO, USA) except for antibiotics ...
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bioRxiv - Microbiology 2019Quote: ... and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’) (Sigma, UK).
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bioRxiv - Microbiology 2020Quote: ... the following fragments were amplified using specific primers and cloned in pET24a (Novagen) using the NdeI/XhoI restrictions sites ...
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bioRxiv - Cancer Biology 2022Quote: ... were procured from KiCqStart™ primers (H_GAPDH3_3, H_P4HA2_1 and H_P4HA2_2, Sigma-Aldrich, USA). mRNA expression levels of above-mentioned genes were estimated using iTaq™ Universal SYBR® Green One-step qRT-PCR kit (#1725151 ...
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bioRxiv - Neuroscience 2022Quote: ... 400nM of each gene specific primer HPLC purified (Sigma-Aldrich, St. Louis, MO) in 25 μL reaction volume ...
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bioRxiv - Neuroscience 2020Quote: ... Specific primer pairs were commercially synthesized (Sigma-Aldrich/Merck, Germany; see Table 1). qPCR assays were performed in triplicate reactions using the PowerUp™ SYBR™ Green Master Mix (2X ...
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bioRxiv - Biochemistry 2020Quote: ... Results were also verified with DNA and RNA primers purchased from Sigma-Aldrich Merck KGaA ...
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bioRxiv - Developmental Biology 2019Quote: ... Gene expression analysis was performed using KiCqStart SYBR Green predesigned primers (Sigma-Aldrich).