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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Systems Biology 2024Quote: ... followed by filtration using 96-Well protein precipitation filter plate (Sigma-Aldrich, 55263-U). Retention index (RI ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations were determined by using a SpectraMax QuickDrop and bovine serum albumin (Sigma) as the reference standards for analysis.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore, Burlington, MA) overnight at 4°C and blocked in 5% nonfat powdered milk in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Genomics 2024Quote: ... Immunoprecipitated complexes were captured using a mix of Protein A (16-661, Sigma-Aldrich) and Protein G magnetic beads (16-662 ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were incubated with 10 times excess of nitroxide spin label MTSSL (Sigma-Aldrich) reagent overnight at 8°C with gentle shaking ...
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bioRxiv - Neuroscience 2024Quote: ... and then proteins were transferred to an Immobilon PVDF membrane (EMD Millipore, Temecula, CA) at 25V for 90 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The protein complex was eluted using 3 mg/ml maltose (Sigma-Aldrich®; USA). Eluted proteins were denatured and separated on SDS-PAGE gel and analyzed by immunoblotting with anti-GST antibody (1:4000 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein complex was eluted using 2.8 mg/ml reduced glutathione (Sigma-Aldrich®; USA). Alternatively ...
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bioRxiv - Molecular Biology 2024Quote: Cellular and aortic tissue proteins were extracted using RIPA buffer (Sigma-Aldrich, catalog #R0278) supplemented with 1% protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins reacted with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore, Burlington, MA, USA), and bands were detected with ImageQuantTM LAS4000 (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking of unspecific protein-antibody interactions was performed with 20% human AB serum (Sigma) in PBS for one hour at RT in the dark ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins with a 6xHis tag were detected with an α-His antibody (Novagen, #70796) in a 1:1000 dilution ...
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bioRxiv - Physiology 2024Quote: ... Immunoblots of RV protein extracts (25 μg) using antibodies to α-tubulin (Sigma-Aldrich:T6199), β-tubulin (Sigma-Aldrich:T4026) ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands were detected by an enhanced chemiluminescence (ECL) kit (Millipore, Billerica, MA, USA) using a Chemiluminescence Analyzer (Chemiscope600pro ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µL of 1/5 diluted protein extracts and 25µL of Bradford reactive (Sigma) were placed in a 96-well microtiter plate and incubated in a Synergy 2 Multi-Mode Reader (BioTek ...
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bioRxiv - Plant Biology 2024Quote: ... Dynabeads Protein G were conjugated with a FLAG antibody (Sigma; F1804; diluted 1:1,000).
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bioRxiv - Molecular Biology 2024Quote: ... one milligram of total proteins was immunoprecipitated with anti-Flag affinity gel (Sigma-Aldrich) in the following NT2 buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The VDE protein was inhibited by infiltration with 5 mM dithiothreitol (DTT, Sigma Aldrich). As a control ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Biophysics 2024Quote: ... Protein bands were transferred by tank transfer onto a polyvinylidene difluoride (PVDF, EMD Millipore) membrane in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced with 20 mM DL-Dithiothreitol (DTT; BioUltra, for molecular biology, Sigma) at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to PVDF membranes (pore size 0.45 µm, Immobilon-P, Merck Millipore) using Trans-Blot Turbo Blotting System (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... and GST-tagged proteins were purified using pre-hydrated glutathione agarose beads (Sigma Aldrich). His6-tagged proteins were eluted by sequential washes with imidazole-containing buffers at increasing concentrations (10 mM ...
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bioRxiv - Physiology 2024Quote: ... protein content was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.) and bovine serum albumin as a standard (Millipore Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein concentration determination was carried out using the bicinchoninic acid assay kit (Sigma-Aldrich) and using BSA as a standard ...
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Development of ketobenzothiazole-based peptidomimetic TMPRSS13 inhibitors with low nanomolar potencybioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were then concentrated using Amicon Ultra centrifugal filter 10 kDa (Millipore Sigma, UFC8010) and kept at −80°C.
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bioRxiv - Plant Biology 2024Quote: ... followed by incubation with 25 µL of Protein G Sepharose beads (Sigma-Aldrich®) for 2 h at 4 °C (Yang et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to the beads were eluted with 3×FLAG peptide (Sigma, F4799) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3× FLAG Peptide (Sigma F4799) diluted in Tris-buffer saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... specific protein bands were developed using the Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) for 3 min ...
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bioRxiv - Microbiology 2024Quote: Proteins from cell lysates were reduced using 5 mM dithiothreitol (DTT, Sigma-Aldrich #43815) for 30 min at 25°C with agitation ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced by adding 2.8 µL of 250 mM dithiothreitol (Sigma-Aldrich, USA) and incubated at 37°C for 45 min while shaking at 400 rpm on a block heater ...
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bioRxiv - Biophysics 2024Quote: ... the protein expression was induced by adding 10 mM sodium butyrate (Sigma-Aldrich, 303410) and transferring the cells to an incubator set at 30 °C ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were transferred to Immobilon PVDF membranes (pore size 0.45 μM, Merck Millipore IPVH00010) for 1 h at 100 V ...
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bioRxiv - Synthetic Biology 2024Quote: ... Eluted protein was concentrated in a 10 kDa MWCO Amicon spin filter (EMD Millipore) to < 1 mL ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP was detected using an anti-green Fluorescent Protein (GFP) Monoclonal antibody (SIGMA®) at a 1:2,500 dilution in 0.1% PBS-Tween 5% skimmed milk ...
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bioRxiv - Bioengineering 2024Quote: ... and then the proteins were transferred to polyvinylidene difluoride (PVDF, Cat. No. IPVH00010, Millipore) membrane ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted fractions containing pure FLuc were concentrated using Vivaspin 50 kDa protein concentrators (Sigma) and injected directly onto a 16/60 Superdex 200 pg column (Cytiva ...