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Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff, Millipore) and further purified using Superose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich). The membrane was then blocked using 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 μL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Genetics 2021Quote: ... Proteins were blotted from the gel onto polyvinylidene difluoride (PVDF) membranes (Immobilon, EMD Millipore) and detected using primary and secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted according to protocols described before (42) ...
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bioRxiv - Microbiology 2021Quote: The proteins in the elution were concentrated by Amicon Ultra centrifugal filters (Millipore Sigma) and separated briefly in a 10% Bis-Tris SDS-PAGE gel for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... The resultant protein bands were visualized after staining with Ponceau-S (Sigma-Aldrich, USA). Next ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Purkinje cell protein 4 (PCP4; Sigma, dilution 1:400), rabbit polyclonal antibody raised to Capn15 (Zha ...
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bioRxiv - Immunology 2020Quote: ... ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578, Sigma-Aldrich, Oakville, Ontario) at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Excess Protein-PEG2000-DPSE was separated from liposomes using a CL-4B Sepharose (Sigma) column ...
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bioRxiv - Cell Biology 2020Quote: ... Primary and secondary antibodies were diluted as follows: polyclonal rabbit-anti-Protein A (Sigma) detecting the PTP epitope 1:2000 ...
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bioRxiv - Immunology 2021Quote: Actin was used as a housekeeping protein (Rabbit anti-Actin antibody (Sigma-Aldrich A2066) followed by Goat anti-Rabbit-HRP (Southern Biotech 4050-05)).
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bioRxiv - Molecular Biology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted as described43 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then eluted with lysis buffer containing imidazole (Sigma-Aldrich Co., USA) at different concentrations (20 mM ...
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bioRxiv - Microbiology 2022Quote: ... The protein samples were concentrated by ultrafiltration using an Amicon Ultra-3KDa concentrator (Millipore). For further purification ...
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bioRxiv - Neuroscience 2022Quote: ... Soluble proteins were then incubated with 1 μg of anti-FLAG antibody (F1804, Sigma) or 1 μg of mouse IgG (sc-2025 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysate was then blotting on a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA) and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies.
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra-10k centrifugal filter device (Merck Millipore Ltd.), and the concentration was determined using Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and pre-cleaned with Protein G Agarose beads (Sigma-Aldrich) for 1-2 h at 4 °C rotating to enhance specificity and the total protein content was quantified by Bradford assay ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: We conducted SEC on the SEC protein standards (molecular weights 12.4-200 kDa; Sigma) including β-amylase from sweet potato (200 kDa) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was concentrated by centrifugal filtration (Amicon, EMD Millipore, 10-kDa MW cutoff) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein recovery was maximized by adjusting the concentration of imidazole (Sigma cat# l5513-5G) as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were transferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Sigma-Aldrich) at 100V for 1 hour on ice in NuPAGE Transfer Buffer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-FL Transfer Membrane (Millipore). The membranes were blocked in PBS-T (PBS containing 0.05% Tween 20 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Total cell protein was extracted with lysis buffer (RIPA, Sigma-Aldrich, catalog no. R0278) containing 1% protease-phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Proteins separated by SDS–PAGE were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100 V for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
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bioRxiv - Microbiology 2022Quote: ... the total protein concentration of each sample was measured via Bradford assay (Sigma Aldrich). Samples were subsequently diluted to a final concentration of 0.5 µg total protein/µl ...
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bioRxiv - Microbiology 2022Quote: ... The TS fraction was concentrated using Amicon protein concentrators with a 10KDa cutoff (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Not protein depletions were obtained by addition of auxin (3-indoleacetic acid, Sigma-Aldrich I2886 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
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bioRxiv - Neuroscience 2022Quote: ... and 15 μL of Protein-G Sepharose (Millipore/Cytiva Cat#GE-17-0618-05), washed three times in ice-cold wash buffer (0.1% Triton X-100 in 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the Bradford reagent (Sigma Aldrich, St. Louis, MO, USA). Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by using Trans-Blot Turbo™ Transfer Pack (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
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bioRxiv - Plant Biology 2022Quote: For NR activity measurements with the purified protein the procedure was adapted from Sigma quality control tests for product N7265 based on (Gilliam et al. ...
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bioRxiv - Physiology 2023Quote: ... protein concentrations of pancreatic samples were quantified using a Bradford assay (Sigma-Aldrich, B6916). Insulin and SST values were then normalized to protein concentration ...
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bioRxiv - Plant Biology 2023Quote: ... The peak fraction of each BE protein was desalted on PD10 desalting column (Sigma) equilibrated in 20 mM Tris/HCl pH 7 ...
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bioRxiv - Genomics 2022Quote: HC protein fragments were cleaved by thrombin (40 mU/μL) (EMD Millipore, 605157-1KU) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...