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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse or anti-rabbit secondary antibodies coupled to peroxidase were from Sigma.
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bioRxiv - Biochemistry 2024Quote: ... The membranes were incubated with mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, F1804) or rabbit polyclonal anti-GFP (life technologies ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-polyHis monoclonal primary antibodies (Mouse; Sigma Aldrich; Extended Data Fig. 9d) followed by alkaline phosphatase conjugated anti-goat or anti-mouse secondary antibodies (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include mouse IgG anti-acetylated-tubulin (1:1,000; Sigma-Aldrich, T6793), rabbit anti-Centrin 1 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... or monoclonal anti-mouse ß- actin (1:5000; clone AC-15, Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: Immortalized mouse inner medullary collecting duct (mIMCD3) cells were cultured in DMEM:F12 (Sigma) supplemented with GlutaMAX (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Mouse anti VSV G (SAB4200695, dilution 1/5000) was purchased from Sigma-Aldrich and used for western blot analysis ...
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bioRxiv - Cell Biology 2024Quote: ... mouse IgG (Becton Dickinson) of rabbit IgG (Merck Millipore, New Jersey, United States) was complexed with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were stained with mouse anti-LH2AX (Millipore-Sigma, St.Louis, MO, 05-636) at 1:500 and rabbit anti-RPA (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were stained with mouse anti-LH2AX (Millipore-Sigma, St.Louis, MO, 05-636) at 1:500 and rabbit anti-RPA (Bethyl Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-vinculin (clone hVin-1, 1:2,000 for WB; V9264; Sigma-Aldrich). Secondary Alexa Fluor 680– conjugated (1:10,000 ...
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bioRxiv - Pathology 2024Quote: ... and mouse α-Tubulin (1:5000; T5168, Sigma-Aldrich; Saint Louis, MO, USA). Proteins on membranes were visualized using the chemiluminescence detection kit Immobilon Crescendo Western HRP substrate (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated with Amicon® 3 kDa MWCO concentrator (Millipore, Burlington, MA) and injected into a 10/300 Superdex 75 size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg of protein was loaded into a 10 kDa centrifugal filter tube (Millipore), washed twice with 200 μl of UA buffer (8 M urea in 0.1 M Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved using SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Membranes were blocked using Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Cell Biology 2020Quote: ... protein translation was briefly inhibited by treatment with 20 mg/mL cycloheximide (CHX; Sigma) for 8 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Systems Biology 2020Quote: ... Particle-bound proteins were transferred to a filter plate (Millipore MultiScreen, 0.22 µm, MSGVN2250) and centrifuged (1000 g ...
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bioRxiv - Developmental Biology 2021Quote: ... 70µg total protein per sample was reduced with tris-2-carboxyethyl phosphine (TCEP, Sigma) for 1 hour at 60°C at a final concentration of 5mM ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St Louis, MO) as per manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Millipore, Cork, Ireland), which were blocked with a 2:1 ratio of Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of proteins was incubated with anti-HA affinity matrix (Sigma, #11815016001) to pull-down SMO-HA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by 0.2 mM Isopropyl-ß-D-thiogalactopyranoside (IPTG; Sigma-Aldrich) and continued at 20°C for 20 h ...
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bioRxiv - Cell Biology 2020Quote: ... isolated heart mitochondria were solubilized with digitonin (Sigma Aldrich, 6g/g digitonin/protein ratio) and resolved on a 4-12% Bis-Tris polyacrylamide gradient gel (Genscript) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was incubated with Protein G-coupled magnetic beads (Sigma, MO, USA) that captured with anti-GFP for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein quantification was carried out using BCA (Bicinchoninic Acid, Sigma-Aldrich, St. Louis, MO) method taking bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were expressed in Sf9 insect cells and purified on M2 agarose (Sigma Aldrich) as described (J ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Molecular Biology 2022Quote: Crude protein extracts were mixed with 1% Sodium Dodecyl Sulphate (SDS; Sigma-Aldrich, USA) and boiled for 1 min ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Cell Biology 2022Quote: ... were coated with the following ECM proteins when noted: gelatin (Sigma Aldrich, catalog # G2500) (0.1% (w/v ...
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bioRxiv - Genetics 2022Quote: ... Immune complexes were collected by centrifugation and cleared using Protein A agarose beads (Millipore) and incubated for 1.5 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated using a 10 kDa molecular weight cut-off concentrator (Millipore) and loaded onto a HiLoad 16/600 Superdex 75 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of Magna ChIP protein A magnetic beads (16–661, Millipore, Molsheim, France) were added for 1h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was concentrated with a 30-kDa molecular mass cut-off concentrator (Millipore) by centrifugation for 5 min at 2,500 x g ...
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bioRxiv - Developmental Biology 2022Quote: ... and separated proteins were transfer to polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Fisher Scientific). Total lane protein was then determined via Ponceau stain (0.1% Ponceau-S in 5% acetic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Small molecules/ proteins were added to extracts as follows: 1 µM MLN8237 (Sigma-Aldrich) (or 0.5% DMSO ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was concentrated by ultrafiltration using an Amicon 50K filter unit (Millipore). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were analyzed on SDS-PAGE and concentrated with Amicon Ultra concentrators (Millipore), aliquoted and stored at −80 °C ...
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bioRxiv - Physiology 2020Quote: ... The protein concentration was assessed using bicinchoninic acid assay (BCA, BCA1-1KT, Sigma Aldrich). A total of 10-40 μg of protein was loaded on 8-14% acrylamide gel ...
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bioRxiv - Immunology 2020Quote: ... Proteins were then transferred onto a PVDF membrane (Immobilon®-P Transfer Membrane, Millipore) via wet transfer and blocked in 5% skim milk/Tris-buffered saline (TBST ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted the soluble protein from the damselflies using 0.1 M NaOH (Sigma-Aldrich) as a lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...