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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: HC protein fragments were cleaved by thrombin (40 mU/μL) (EMD Millipore, 605157-1KU) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30kDa MWCO concentrator (EMD Millipore) to 1mL ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated and transferred to Immobilon-P PVDF membrane (0.45 μm, Millipore Sigma). The membrane was blocked with 5% bovine serum albumin for 1 h and incubated overnight at 4°C with various antibodies (1:500 dilution for the PIKfyve antibody and 1:1000 dilution for all other antibodies) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a PVDF membrane (Millipore; Immobilon-FL, 0.45 mm pore size) in transfer buffer (25 mM tris ...
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bioRxiv - Biophysics 2023Quote: ... then the proteins were stained with 0.22% Coumassie brilliant blue G-250 (Sigma, USA) diluted in the solution F ...
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bioRxiv - Bioengineering 2023Quote: ... then resuspended in 100 µL protein storage buffer: 4 M urea (Sigma-Aldrich, U5128), 1 % SDS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Cell Biology 2022Quote: ... For Chemiluminescence westerns: The proteins were electro-transferred to PVDF membranes (0.45 μm, Millipore), and then the total protein was stained using Ponceau (Thermo Fisher Scientific) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... Protein transfer was performed on methanol-activated Immobilon®-FL PVDF membrane (Sigma-Aldrich). Membrane was blocked for 1h in 5% BSA (bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of pure protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) was injected subcutaneously to six weeks old female NMRI mice (Charles River Laboratories ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a nitrocellulose membrane and stained with Ponceau S (Sigma Aldrich) to monitor protein loading ...
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bioRxiv - Biochemistry 2023Quote: ... Expression of chaperone protein was induced with 1 μg/ml tetracycline (Sigma-Aldrich, T7660).
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding 0.5 mM isopropyl β-Dthiogalactopyranoside (IPTG; Sigma Aldrich). After 20h incubation at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Biochemistry 2023Quote: ... the protein was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 41.46 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... Then proteins were transferred onto a 0.45-μm polyvinylidene fluoride membrane (Sigma Aldrich, IEVH00005) and the membranes were blocked in TBST containing 5% skimmed milk for 1 h at room temperature and then incubated with the indicated primary antibodies overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-V5 or GFP-FLAG fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich). Forty-eight hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Cancer Biology 2023Quote: ... Separated proteins were then transferred to Immobilon®-P PVDF Membranes (Milipore Sigma #ISEQ00010) using the BioRad Transblot SD Semi-dry Transfer Cell as per manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred to an Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and the membranes were incubated with Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were similarly incubated with HA binding protein (HABP) (EMD Millipore 385911, Burlington, MA), then detected with an appropriate fluorescent secondary antibody for 1 h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...
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bioRxiv - Biophysics 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 8.4 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Solubilized protein was added to pre-equilibrated anti-FLAG agarose affinity resin (Sigma, A2220) and incubated for 90min at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced with 20 mM DL-Dithiothreitol (DTT; BioUltra, for molecular biology, Sigma) at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore, Burlington, MA) overnight at 4°C and blocked in 5% nonfat powdered milk in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... and then proteins were transferred to an Immobilon PVDF membrane (EMD Millipore, Temecula, CA) at 25V for 90 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to PVDF membranes (pore size 0.45 µm, Immobilon-P, Merck Millipore) using Trans-Blot Turbo Blotting System (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins reacted with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore, Burlington, MA, USA), and bands were detected with ImageQuantTM LAS4000 (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to the beads were eluted with 3×FLAG peptide (Sigma, F4799) for 30 min at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Immunoprecipitated complexes were captured using a mix of Protein A (16-661, Sigma-Aldrich) and Protein G magnetic beads (16-662 ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were incubated with 10 times excess of nitroxide spin label MTSSL (Sigma-Aldrich) reagent overnight at 8°C with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... specific protein bands were developed using the Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) for 3 min ...