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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 30-40µg of the total protein was combined with 2X sample buffer (Sigma-Aldrich) and boiled for 10 mins before loading in 11% SDS-PAGE ...
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bioRxiv - Biophysics 2020Quote: ... pH 7) and filtration of the labeled protein through 0.22 µm filter (Millex, Millipore).
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bioRxiv - Neuroscience 2022Quote: ... Protein was diluted in Laemmli sample buffer with 5% 2-Mercaptoethanol (Sigma-Aldrich M6250) and denatured for 5 mins at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... The protein samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 3% BSA + 0.05% Tween-20 for 1 h before incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA).
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bioRxiv - Immunology 2022Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
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bioRxiv - Genetics 2022Quote: ... Protein bands were developed with Immobilon Western Chemiluminescent HRP Substrate solutions (Millipore Sigma #WBKLS0050) and visualized under UV light using BioRad’s ChemiDoc MP™ Imaging Platform ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were transferred from polyacrylamide gel onto a polyvinylidene difluoride membrane (Millipore IPVH00010). After the transfer ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted from pooled beads with 0.5mg/ml FLAG peptide (#F3290, Millipore Sigma) by shaking at 1000 rpm for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-P; Merck Millipore, MA, USA) using a Trans-Blot® SD semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of GFP-SGS3 protein was induced in Escherichia coli Rosetta (DE3) (Novagen) by adding 0.1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated in a 10k MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) and loaded onto a Superdex 200 HiLoad 16/60 gel filtration column equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... We induce protein expression by adding 1 mM isopropyl β-d-1-thiogalactopyranoside (Sigma) when the cell density reaches an ODλ=600nm of 0.6 ~ 0.7 ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2021Quote: ... the specific proteins were visualized with a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins for size-exclusion column calibration were purchased from Sigma-Aldrich (St. Louis, MO). 5-aminolevulinic acid was purchased from Carbosynth (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were transferred to PVDF (polyvinylidene difluoride) membranes (0.2 μm pore size, Millipore) by immersion transfer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Protein lysates were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Millipore). After the membranes were blocked using 5% skimmed milk (Megmilk Snow Brand ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was concentrated to 5 mg·ml−1 in an Amicon ultracentrifugation device (Millipore) with 10 kDa cutoff and directly used for further studies or supplemented with 40% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... A pre-clear step was performed by adding protein A-agarose beads (Merck Millipore) to the lysates ...
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bioRxiv - Biochemistry 2020Quote: Solutions of intact and deglycosylated proteins were passed through the ZipTip C18 microcolumns (Millipore), washed and eluted according to manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Bioengineering 2020Quote: ... Proteins were then reduced with 10 mM DTT/40 mM NH4HCO3 (Sigma-Aldrich Co.) at 56°C for 20 min ...
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bioRxiv - Genetics 2020Quote: The GST-Clr4 fusion protein was allowed to bind to Glutathione-agarose beads (Sigma) in MTPBS buffer (100mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... The purified VxrB protein was concentrated by centrifugal filter devices (Millipore, 3,000 MW CO) before injection onto High load TM (16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of each sample was precleared with EZview protein G beads (Sigma-Aldrich) for 0.5 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were pre-cleared by incubation with 40 μl Protein A dynabeads (Sigma) for one hour at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The collected protein fractions were pooled and concentrated using an Amicon ultrafiltration cell (Millipore) and dialyzed into PBS (10 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: Proteins resolved through SDS-PAGE gels were transferred to Immobilon-P PVDF membranes (Millipore) and blocked with 3% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were ultrafiltrated using 10 K pore size Amicon Ultra filter units (Millipore) and step-wise dialyzed against 5 mM Pipes-Na pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was induced with 0.25 mM IPTG in E.coli Rosetta BL21(DE3) (Novagen) in the presence of 50 µM ZnCl2 at 37° C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 55 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The secreted S proteins were purified by anti-FLAG M2 affinity resin (Sigma Aldrich). After loading two times ...
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bioRxiv - Cell Biology 2021Quote: ... we inhibited global protein synthesis with cycloheximide (CHX, 100 μg/mL, Sigma Aldrich # C4859).
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins were extracted from transfected 293T cells using RIPA lysis buffer (Millipore Sigma) at 48 hpt ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 mg of protein was mixed with 2x Laemmli buffer (Sigma Aldrich, #S3401) and boiled for 5 min at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then electrotransferred to an Immobilon®-P polyvinylidene fluoride (PVDF) membrane (Millipore). Membrane was blocked with 5% non-fat milk ...
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bioRxiv - Genomics 2021Quote: Proteins were separated using 6-15% SDS-PAGE and transferred onto PVDF membranes (Millipore). Then ...