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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore, cat. no. IPVH00010, Jerusalem, Israel). Prior to antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... and proteins were electrophoretically transferred onto methanol-activated polyvinylidene fluoride (PVDF) microporous membranes (Millipore). Membranes were blocked for one hour with 5% bovine serum albumin (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The interaction of the proteins was quantified after adding the substrate phenylenediaminedihydrochloride (Sigma Aldrich) plus H2O2 by measuring the resulting absorbance at 492 nm in the Multiskan SkyHigh Spectrophotometer (ThermoFisher Sientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Flag-tagged protein was purified using 20µl anti-Flag® M2 magnetic beads (Sigma) per plate ...
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bioRxiv - Cell Biology 2024Quote: ... the specific proteins were visualized using a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) and LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were then digested with 10 µg/mL proteinase K (Sigma-Aldrich) at 55 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins on gels were transferred on PVDF membrane (Immobilon-P, Millipore, Cat no. IPVH00010) and blocked with 5% non-fat dry milk (Scientific ...
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bioRxiv - Pathology 2022Quote: ... the incubated protein mixture was collected and centrifuged using centrifugal filters Utracel-100K (Millipore) at 500 × g for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using 10 kDa cutoff Amicon Ultra Centrifugal Filters (Merck Millipore) and stored in 50% v/v glycerol at -20 °C until further use.
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bioRxiv - Pathology 2024Quote: ... the resolved protein samples were transferred onto a PVDF membrane (Merck Millipore Ltd., USA) through a Bio-Rad electrophoretic blotting apparatus ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore). The protein concentration was measured on a Nanodrop 1000 spectrophotometer at 280 nm using the extinction coefficient of Trp and Tyr residues in the GST-LC3B sequence (48820 cm-1M-1).
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were semi-dry blotted onto a 0.2 µM nitrocellulose membrane (Sigma cat GE10600001) and protein levels were detected by specific antibodies directed against ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30K MWCO concentrator (EMD Millipore) to 1 ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore). All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
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bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
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bioRxiv - Cell Biology 2024Quote: mESC protein samples were isolated from mESC pellets using ice-cold RIPA buffer (Sigma) in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 protein was extracted from cells using RIPA buffer (Sigma-Aldrich, UK. Cat # R0278) using the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... biotinylated proteins bound to streptavidin beads were resuspended in 100 mM ammonium bicarbonate (Sigma) and digested with 2 µg Trypsin (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The eluted proteins were further concentrated using Amicon® Ultra-4 centrifugal filter (Millipore) and contaminants were removed using Gel-filtration chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µg of proteins were reduced with 10 mM dithiothreitol (Sigma-Aldrich, MO, USA) at 37 °C for 60 min ...
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bioRxiv - Neuroscience 2024Quote: Proteins was isolated from cultured cells or brain tissues with RIPA buffer (Sigma, R0278) including protease inhibitors cocktails ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were eluted with wash buffer containing 1.5% β-mercaptoethanol (Sigma-Aldrich, M3148) at 37 °C for 0.5 h with agitation (350 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto 0.45 nm pore polyvinylidene fluoride (PVDF) membrane (Millipore, USA) using an eBlot™ L1 wet transfer (GenScript Biotech ...
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bioRxiv - Immunology 2024Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma). All blots were run at least twice to check for reproducibility ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Genetics 2023Quote: ... 20 μL Magna ChIP™ Protein A+G Magnetic Beads (Sigma-Aldrich 16-663) was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody ...
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bioRxiv - Genetics 2023Quote: ... and the supernatants were precleared with 10 μL of Protein A-coated agarose (Millipore). The precleared supernatants were then divided into two equal parts and incubated with 20 μL 14-3-3 polyclonal antibody and IgG overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
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bioRxiv - Evolutionary Biology 2024Quote: ... The crude protein lysate concentration was quantified by bovine serum albumin (BSA) (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were concentrated using 10 kDa cut off Amicon centrifugal filters (Millipore, USA).
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bioRxiv - Bioengineering 2024Quote: ... Proteins were concentrated by centrifugation through Amicon Ultra 15 filter units (Millipore, Burlington, MA) with a 30-kD cutoff and then dialyzed against 3×PBS ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Pathology 2023Quote: Protein was extracted by 1x RIPA lysis buffer (10x, stock solution, EMD Millipore, MA) from mouse stomach tissue ...
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bioRxiv - Plant Biology 2023Quote: Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore Corporation, Billerica, MA, USA) via semi-dry Western blotting ...
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Dynamics of the epigenetic landscape during development and in response to drought stress in sorghumbioRxiv - Plant Biology 2023Quote: ... H2A.Z (preparation using antigen “GKGLLAAKTTAAKSAEKDKGKKAPV”) were incubated with Protein A agarose (16-157, Millipore) before mixed with sonicated chromatin ...
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...